Detection of NDM carbapenemases in klebsiella pneumoniae using the modified method of carbapenemic inactivation

• Autores: Lovera-Garcia, Margarita, Roque-Alcarraz, Mirtha
• Formato: artículo
• Fecha de Publicación: 2021
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/22225
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/22225
• Nivel de acceso: acceso abierto
• Materia: carbapenemases; carbapenem; PCR; Klebsiella pneumoniae NDM; metallobetalactamases; mCIM; carbapenemasas; carbapenémico; metalobetalactamasas

New Delhi-type carbapenemase-producing Klebsiella pneumoniae Metallo-beta-lactamase (NDM) generates a serious public health problem due to its mechanism of antibiotic resistance and rapid dissemination, causing high rates of morbidity and mortality in hospitalized patients when antibiotic therapy options are drained. The objective of the study was to observe the detection capacity of NDM carbapenemases in Klebsiella pneumoniae using the modified carbapenem inactivation method (mCIM). A cross-sectional study was carried out using clinical isolates from a level IV hospital in Lima (Perú) in the months of March to May 2018. It was worked with 40 clinical isolates of Klebsiella pneumoniae resistant to carbapenems, positive for the NDM gene and 15 clinical isolates of Klebsiella pneumoniae sensitive to carbapenems, negative for the NDM gene. The identification of the gene was carried out by conventional PCR. The mCIM in Klebsiella pneumoniae type NDM demonstrated a sensitivity and specificity of 100% and kappa of 1, compared to the PCR. The implementation of the mCIM for the detection of NDM-type carbapenemases in Klebsiella pneumoniae is recommended in laboratories as a fast, easy, low-cost, and timely method for controlling the hospital spread of this type of bacteria.