INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN

Descripción del Articulo

With the objective of the browning controlling enzymatic in fruits, a sequence of experiments of enzymatic inhibition has been developed based on the system enzyme-sustrato-inhibitor (tyrosinase-pyrocatechol-inhibitor).The enzymatic activity of the tyrosinase has been substantially affected by the c...

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Detalles Bibliográficos
Autores: Cjuno H., J., Arroyo C., J.
Formato: artículo
Fecha de Publicación:2009
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/4349
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4349
Nivel de acceso:acceso abierto
Materia:Activity
tyrosinase
pyrocatechol
EDTA
chitosane
papain.
Actividad
tirosinasa
pirocatecol
quitosano
papaína.
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spelling INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAININHIBICIÓN DE LA ACTIVIDAD ENZIMÁTICA DE LA TIROSINASA CON EDTA, QUITOSANO Y PAPAINACjuno H., J.Arroyo C., J.ActivitytyrosinasepyrocatecholEDTAchitosanepapain.ActividadtirosinasapirocatecolEDTAquitosanopapaína.With the objective of the browning controlling enzymatic in fruits, a sequence of experiments of enzymatic inhibition has been developed based on the system enzyme-sustrato-inhibitor (tyrosinase-pyrocatechol-inhibitor).The enzymatic activity of the tyrosinase has been substantially affected by the chelating inhibitors (EDTA), polymeric (chitosane) and enzymatic (papain). The inhibitory effectiveness of the papain has been attributed to its hydrolitic action on the active places 176G and 182E of the tyrosinase. An approach for the constant of Michaelis (Km at 25°C) has given the value of 2,0.Con el objetivo de controlar el pardeamiento enzimático de las frutas, se ha desarrollado una secuencia de experimentos de inhibición enzimática basado en el sistema enzima-sustrato-inhibidor (tirosinasa-pirocatecol-inhibidor). La actividad enzimática de la tirosinasa se ha visto sustancialmente afectada por los inhibidores de quelatación (EDTA), polimérico (quitosano) y enzimático (papaína). La efectividad inhibitoria de la papaína se ha atribuido a su acción hidrolítica sobre los sitios activos 176G y 182E de la tirosinasa. Una aproximación para la constante de Michaelis (Km a 25°C) ha dado el valor de 2,0.Universidad Nacional Mayor de San Marcos2009-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4349Revista Peruana de Química e Ingeniería Química; Vol. 12 No. 2 (2009); 42-48Revista Peruana de Química e Ingeniería Química; Vol. 12 Núm. 2 (2009); 42-481609-75991726-2208reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4349/3475Derechos de autor 2009 J. Cjuno H., J. Arroyo C.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/43492020-05-11T12:28:48Z
dc.title.none.fl_str_mv INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
INHIBICIÓN DE LA ACTIVIDAD ENZIMÁTICA DE LA TIROSINASA CON EDTA, QUITOSANO Y PAPAINA
title INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
spellingShingle INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
Cjuno H., J.
Activity
tyrosinase
pyrocatechol
EDTA
chitosane
papain.
Actividad
tirosinasa
pirocatecol
EDTA
quitosano
papaína.
title_short INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
title_full INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
title_fullStr INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
title_full_unstemmed INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
title_sort INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
dc.creator.none.fl_str_mv Cjuno H., J.
Arroyo C., J.
author Cjuno H., J.
author_facet Cjuno H., J.
Arroyo C., J.
author_role author
author2 Arroyo C., J.
author2_role author
dc.subject.none.fl_str_mv Activity
tyrosinase
pyrocatechol
EDTA
chitosane
papain.
Actividad
tirosinasa
pirocatecol
EDTA
quitosano
papaína.
topic Activity
tyrosinase
pyrocatechol
EDTA
chitosane
papain.
Actividad
tirosinasa
pirocatecol
EDTA
quitosano
papaína.
description With the objective of the browning controlling enzymatic in fruits, a sequence of experiments of enzymatic inhibition has been developed based on the system enzyme-sustrato-inhibitor (tyrosinase-pyrocatechol-inhibitor).The enzymatic activity of the tyrosinase has been substantially affected by the chelating inhibitors (EDTA), polymeric (chitosane) and enzymatic (papain). The inhibitory effectiveness of the papain has been attributed to its hydrolitic action on the active places 176G and 182E of the tyrosinase. An approach for the constant of Michaelis (Km at 25°C) has given the value of 2,0.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4349
url https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4349
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4349/3475
dc.rights.none.fl_str_mv Derechos de autor 2009 J. Cjuno H., J. Arroyo C.
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2009 J. Cjuno H., J. Arroyo C.
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos
dc.source.none.fl_str_mv Revista Peruana de Química e Ingeniería Química; Vol. 12 No. 2 (2009); 42-48
Revista Peruana de Química e Ingeniería Química; Vol. 12 Núm. 2 (2009); 42-48
1609-7599
1726-2208
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238291305398272
score 13.949868
INHIBITION OF ENZYME ACTIVITY OF TYROSINASE WITH EDTA, CHITOSAN AND PAPAIN
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