Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa
Descripción del Articulo
The aim of this study was to evaluate the effect of four separation methods on seminal quality and in vitro fertilizing capacity of equine cryopreserved spermatozoa. Straws of equine cryopreserved semen were used for sperm separation through the Androcoll, CushionFluid, EquiPure and Percoll methods....
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2015 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/11188 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11188 |
| Nivel de acceso: | acceso abierto |
| Materia: | semen quality centrifugation cryopreservation in vitro fertilization calidad seminal centrifugación criopreservación fertilización in vitro |
| id |
REVUNMSM_a7556a943d84c3bef43d14f6fa6606c6 |
|---|---|
| oai_identifier_str |
oai:ojs.csi.unmsm:article/11188 |
| network_acronym_str |
REVUNMSM |
| network_name_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa Efecto de Cuatro Métodos de Separación Seminal sobre la Calidad y la Capacidad Fertilizante in vitro de Espermatozoides Equinos Criopreservados |
| title |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa |
| spellingShingle |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa Varela G., Elizabeth semen quality centrifugation cryopreservation in vitro fertilization calidad seminal centrifugación criopreservación fertilización in vitro |
| title_short |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa |
| title_full |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa |
| title_fullStr |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa |
| title_full_unstemmed |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa |
| title_sort |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoa |
| dc.creator.none.fl_str_mv |
Varela G., Elizabeth Duque C., Juan Esteban Ramírez H., Mónica Ocampo V., Daniel Montoya P., Juan David Restrepo B., Giovanni |
| author |
Varela G., Elizabeth |
| author_facet |
Varela G., Elizabeth Duque C., Juan Esteban Ramírez H., Mónica Ocampo V., Daniel Montoya P., Juan David Restrepo B., Giovanni |
| author_role |
author |
| author2 |
Duque C., Juan Esteban Ramírez H., Mónica Ocampo V., Daniel Montoya P., Juan David Restrepo B., Giovanni |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
semen quality centrifugation cryopreservation in vitro fertilization calidad seminal centrifugación criopreservación fertilización in vitro |
| topic |
semen quality centrifugation cryopreservation in vitro fertilization calidad seminal centrifugación criopreservación fertilización in vitro |
| description |
The aim of this study was to evaluate the effect of four separation methods on seminal quality and in vitro fertilizing capacity of equine cryopreserved spermatozoa. Straws of equine cryopreserved semen were used for sperm separation through the Androcoll, CushionFluid, EquiPure and Percoll methods. A Sperm Class Analyzer (SCA®) system was used to determine total motility, progressive motility, curvilinear velocity, linear velocity and average speed. Also, intact acrosome and sperm vitality were evaluated by fluorescence microscopy. In vitro fertilizing capacity was assessed by in vitro fertilization of bovine oocytes with spermatozoa obtained by each separation method.Cleavage rates were determined after three days of in vitro culture. The results were analyzed using generalized linear models (GLM) and means of the four methods were compared using the Tukey test. The CushionFluid was superior for most parameters of sperm motility as well as for intact acrosome (p<0.05). Higher means of sperm vitality were obtained with EquiPure and Percoll (p<0.05), but no difference were found on cleavage rates between treatments. It was concluded that cryopreserved stallion semen selected by the CushionFluid method had better sperm quality compared to the other three methods; however no difference was evident between them for in vitro fertilizing capacity of spermatozoa. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-09-14 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11188 10.15381/rivep.v26i3.11188 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11188 |
| identifier_str_mv |
10.15381/rivep.v26i3.11188 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11188/10267 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 3 (2015); 451-461 Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 3 (2015); 451-461 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1795238226612453376 |
| spelling |
Effect of four sperm separation methods on quality and in vitro fertilizing capacity of cryopreserved stallion spermatozoaEfecto de Cuatro Métodos de Separación Seminal sobre la Calidad y la Capacidad Fertilizante in vitro de Espermatozoides Equinos CriopreservadosVarela G., ElizabethDuque C., Juan EstebanRamírez H., MónicaOcampo V., DanielMontoya P., Juan DavidRestrepo B., Giovannisemen qualitycentrifugationcryopreservationin vitro fertilizationcalidad seminalcentrifugacióncriopreservaciónfertilización in vitroThe aim of this study was to evaluate the effect of four separation methods on seminal quality and in vitro fertilizing capacity of equine cryopreserved spermatozoa. Straws of equine cryopreserved semen were used for sperm separation through the Androcoll, CushionFluid, EquiPure and Percoll methods. A Sperm Class Analyzer (SCA®) system was used to determine total motility, progressive motility, curvilinear velocity, linear velocity and average speed. Also, intact acrosome and sperm vitality were evaluated by fluorescence microscopy. In vitro fertilizing capacity was assessed by in vitro fertilization of bovine oocytes with spermatozoa obtained by each separation method.Cleavage rates were determined after three days of in vitro culture. The results were analyzed using generalized linear models (GLM) and means of the four methods were compared using the Tukey test. The CushionFluid was superior for most parameters of sperm motility as well as for intact acrosome (p<0.05). Higher means of sperm vitality were obtained with EquiPure and Percoll (p<0.05), but no difference were found on cleavage rates between treatments. It was concluded that cryopreserved stallion semen selected by the CushionFluid method had better sperm quality compared to the other three methods; however no difference was evident between them for in vitro fertilizing capacity of spermatozoa.El objetivo del presente estudio fue evaluar el efecto de cuatro métodos de separación seminal sobre la calidad y la capacidad fertilizante in vitro de espermatozoides equinos criopreservados. Se utilizaron pajillas de semen equino criopreservado para la separación espermática a través de los métodos Androcoll, CushionFluid, EquiPure y Percoll. Mediante un sistema analizador de clase (SCA®) se evaluó la motilidad total, motilidad progresiva, velocidad curvilínea, velocidad lineal y velocidad media, y por microscopía de fluorescencia se determinaron los acrosomas intactos y la vitalidad espermática. La evaluación de la capacidad fertilizante in vitro se realizó mediante la fertilización in vitro de oocitos bovinos con espermatozoides obtenidos en cada método de separación. El clivaje se determinó después de tres días de cultivo in vitro. Los resultados se analizaron mediante modelos lineales generalizados (GLM) y las medias para los diferentes métodos se compararon usando la prueba de Tukey. El CushionFluid fue superior para la mayoría de parámetros de movilidad espermática, así como para acrosomas intactos (p<0.05). Se encontraron medias superiores de vitalidad espermática para EquiPure y Percoll (p<0.05), pero no se encontró diferencia entre las medias de clivaje entre tratamientos. Se concluye que el semen equino criopreservado y seleccionado por el método CushionFluid presenta mejores parámetros de calidad espermática respecto a los otros tres métodos; sin embargo, no se evidencia diferencia entre ellos para la capacidad fertilizante in vitro de los espermatozoides.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2015-09-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1118810.15381/rivep.v26i3.11188Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 3 (2015); 451-461Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 3 (2015); 451-4611682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11188/10267Derechos de autor 2015 Elizabeth Varela G., Juan Esteban Duque C., Mónica Ramírez H., Daniel Ocampo V., Juan David Montoya P., Giovanni Restrepo B.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/111882020-03-29T19:05:57Z |
| score |
13.95948 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
