The aim of this study was to evaluate the effect of four separation methods on seminal quality and in vitro fertilizing capacity of equine cryopreserved spermatozoa. Straws of equine cryopreserved semen were used for sperm separation through the Androcoll, CushionFluid, EquiPure and Percoll methods. A Sperm Class Analyzer (SCA®) system was used to determine total motility, progressive motility, curvilinear velocity, linear velocity and average speed. Also, intact acrosome and sperm vitality were evaluated by fluorescence microscopy. In vitro fertilizing capacity was assessed by in vitro fertilization of bovine oocytes with spermatozoa obtained by each separation method.Cleavage rates were determined after three days of in vitro culture. The results were analyzed using generalized linear models (GLM) and means of the four methods were compared using the Tukey test. The CushionFluid was s...

The aim of this study was to evaluate the enzymatic antioxidant capacity of cryopreserved bovine semen with different sources of low-density lipoproteins (LDL) and trehalose (T). Ten ejaculates selected from five bulls were cryopreserved under the treatments: LDL (8% m/v), T (100 mM), TLDL (100 mM T and 8% m/v LDL), centrifuged egg yolk (CEY, 20% v/v) and egg yolk (EY, 20% v/v) as control treatment. After the semen thawing process, the antioxidant enzymatic capacity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) of bovine semen were evaluated by spectrophotometry plus its relationship with spermatic kinetic parameters. A completely random model was used. The normality of the variables was validated by the Kolmogorov- Smirnov test. The sources of variation were evaluated through a mixed model. The comparison of the adjusted means between the treatments was ...

The aim of this study was to evaluate the enzymatic antioxidant capacity of cryopreserved bovine semen with different sources of low-density lipoproteins (LDL) and trehalose (T). Ten ejaculates selected from five bulls were cryopreserved under the treatments: LDL (8% m/v), T (100 mM), TLDL (100 mM T and 8% m/v LDL), centrifuged egg yolk (CEY, 20% v/v) and egg yolk (EY, 20% v/v) as control treatment. After the semen thawing process, the antioxidant enzymatic capacity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) of bovine semen were evaluated by spectrophotometry plus its relationship with spermatic kinetic parameters. A completely random model was used. The normality of the variables was validated by the Kolmogorov- Smirnov test. The sources of variation were evaluated through a mixed model. The comparison of the adjusted means between the treatments was ...

The aim of this study was to evaluate the effect of four separation methods on seminal quality and in vitro fertilizing capacity of equine cryopreserved spermatozoa. Straws of equine cryopreserved semen were used for sperm separation through the Androcoll, CushionFluid, EquiPure and Percoll methods. A Sperm Class Analyzer (SCA®) system was used to determine total motility, progressive motility, curvilinear velocity, linear velocity and average speed. Also, intact acrosome and sperm vitality were evaluated by fluorescence microscopy. In vitro fertilizing capacity was assessed by in vitro fertilization of bovine oocytes with spermatozoa obtained by each separation method.Cleavage rates were determined after three days of in vitro culture. The results were analyzed using generalized linear models (GLM) and means of the four methods were compared using the Tukey test. The CushionFluid was s...