Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca

Descripción del Articulo

Cuniculus paca is a terrestrial rodent that inhabits the neotropical forests of Central and South America. The aim of this study was to evaluate the effect of sperm separation on the quality of cryopreserved semen of C. paca. Sixty straws of frozen semen were thawed. One aliquot was subjected to spe...

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Detalles Bibliográficos
Autores: Munera C., Daniela, Restrepo B., Giovanni
Formato: artículo
Fecha de Publicación:2023
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/26377
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/26377
Nivel de acceso:acceso abierto
Materia:Cuniculus paca
spermatozoa
seminal separation
sperm quality
espermatozoides
separación seminal
calidad espermática
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spelling Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus pacaEfecto de la separación por gradiente en una sola capa sobre la calidad de espermatozoides criopreservados de Cuniculus pacaMunera C., DanielaRestrepo B., GiovanniMunera C., DanielaRestrepo B., GiovanniCuniculus pacaspermatozoaseminal separationsperm qualityCuniculus pacaespermatozoidesseparación seminalcalidad espermáticaCuniculus paca is a terrestrial rodent that inhabits the neotropical forests of Central and South America. The aim of this study was to evaluate the effect of sperm separation on the quality of cryopreserved semen of C. paca. Sixty straws of frozen semen were thawed. One aliquot was subjected to sperm separation by single-layer density gradient centrifugation (SLC) and the other aliquot remained as a control group with semen not separated. Sperm motility and kinetics were evaluated using a computerized-assisted sperm analysis (CASA) system, functional membrane integrity by hypoosmotic test (HOST), and sperm morphology by eosin-nigrosin staining. The adjustment of linear models and comparison of means by Tukey was performed. The SLC separation produced a higher proportion of motile and linear spermatozoa, while the control showed higher values for curvilinear velocity (p˂0.05). Likewise, the SLC separation produced superior results for the vitality and functional integrity of the membrane (p˂0.05). It is concluded that the SLC separation of cryopreserved semen of C. paca allows obtaining samples with superior sperm quality characteristics, which could be used in repopulation programmes through assisted reproduction for this species.Cuniculus paca es un roedor terrestre que habita los bosques neotrópicos de centro y Suramérica. El objetivo de este estudio fue evaluar el efecto de la separación espermática sobre la calidad de semen criopreservado de C. paca. Sesenta pajillas de semen congelado fueron descongeladas. Una alícuota fue sometida a separación espermática por centrifugación con gradiente de densidad de una capa (SLC) y la otra alícuota permaneció como grupo control con semen no separado. Se evaluó la movilidad y cinética espermática mediante un sistema computarizado de análisis espermático (CASA), la integridad funcional de la membrana por la prueba hipoosmótica (HOST) y la morfología espermática con la tinción con eosina-nigrosina. Se realizó el ajuste de modelos lineales y comparación de medias por Tukey. La separación SLC produjo mayor proporción de espermatozoides móviles y lineales, mientras que el control mostró valores superiores para la velocidad curvilínea (p˂0.05). Asimismo, la separación SLC produjo resultados superiores para la vitalidad y la integridad funcional de la membrana (p˂0.05). Se concluye que la separación SLC de semen criopreservado de C. paca permite la obtención de muestras con características superiores de calidad espermática que podría utilizarse en programas de repoblamiento a través de reproducción asistida para esta especie.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2023-10-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2637710.15381/rivep.v34i5.26377Revista de Investigaciones Veterinarias del Perú; Vol. 34 Núm. 5 (2023); e26377Revista de Investigaciones Veterinarias del Perú; Vol. 34 No. 5 (2023); e263771682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/26377/20255Derechos de autor 2023 Daniela Munera C., Giovanni Restrepo B.http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/263772023-11-01T05:20:37Z
dc.title.none.fl_str_mv Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
Efecto de la separación por gradiente en una sola capa sobre la calidad de espermatozoides criopreservados de Cuniculus paca
title Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
spellingShingle Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
Munera C., Daniela
Cuniculus paca
spermatozoa
seminal separation
sperm quality
Cuniculus paca
espermatozoides
separación seminal
calidad espermática
title_short Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
title_full Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
title_fullStr Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
title_full_unstemmed Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
title_sort Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
dc.creator.none.fl_str_mv Munera C., Daniela
Restrepo B., Giovanni
Munera C., Daniela
Restrepo B., Giovanni
author Munera C., Daniela
author_facet Munera C., Daniela
Restrepo B., Giovanni
author_role author
author2 Restrepo B., Giovanni
author2_role author
dc.subject.none.fl_str_mv Cuniculus paca
spermatozoa
seminal separation
sperm quality
Cuniculus paca
espermatozoides
separación seminal
calidad espermática
topic Cuniculus paca
spermatozoa
seminal separation
sperm quality
Cuniculus paca
espermatozoides
separación seminal
calidad espermática
description Cuniculus paca is a terrestrial rodent that inhabits the neotropical forests of Central and South America. The aim of this study was to evaluate the effect of sperm separation on the quality of cryopreserved semen of C. paca. Sixty straws of frozen semen were thawed. One aliquot was subjected to sperm separation by single-layer density gradient centrifugation (SLC) and the other aliquot remained as a control group with semen not separated. Sperm motility and kinetics were evaluated using a computerized-assisted sperm analysis (CASA) system, functional membrane integrity by hypoosmotic test (HOST), and sperm morphology by eosin-nigrosin staining. The adjustment of linear models and comparison of means by Tukey was performed. The SLC separation produced a higher proportion of motile and linear spermatozoa, while the control showed higher values for curvilinear velocity (p˂0.05). Likewise, the SLC separation produced superior results for the vitality and functional integrity of the membrane (p˂0.05). It is concluded that the SLC separation of cryopreserved semen of C. paca allows obtaining samples with superior sperm quality characteristics, which could be used in repopulation programmes through assisted reproduction for this species.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/26377
10.15381/rivep.v34i5.26377
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/26377
identifier_str_mv 10.15381/rivep.v34i5.26377
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/26377/20255
dc.rights.none.fl_str_mv Derechos de autor 2023 Daniela Munera C., Giovanni Restrepo B.
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2023 Daniela Munera C., Giovanni Restrepo B.
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 34 Núm. 5 (2023); e26377
Revista de Investigaciones Veterinarias del Perú; Vol. 34 No. 5 (2023); e26377
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.987529
Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
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