Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation

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This study aimed to evaluate the efficiency of embryo recovery in single ovulation, the quality of the embryos and the interspecies fertility through embryo transfer. Nine donor alpacas and 40 recipient llamas were used. Ultrasonography of the ovaries was done to determine the presence of an ovulato...

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Detalles Bibliográficos
Autores: Pacheco, Joel, Vélez, Víctor, Pezo, Danilo
Formato: artículo
Fecha de Publicación:2016
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/11464
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11464
Nivel de acceso:acceso abierto
Materia:embryos
interspecies transfer
simple ovulation
embriones
transferencia interespecie
ovulación simple
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spelling Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulationEvaluación de la Eficiencia de la Transferencia de Embriones Interespecie entre Alpacas y Llamas Obtenidos por Ovulación SimplePacheco, JoelVélez, VíctorPezo, Daniloembryosinterspecies transfersimple ovulationembrionestransferencia interespecieovulación simpleThis study aimed to evaluate the efficiency of embryo recovery in single ovulation, the quality of the embryos and the interspecies fertility through embryo transfer. Nine donor alpacas and 40 recipient llamas were used. Ultrasonography of the ovaries was done to determine the presence of an ovulatory follicle. Donors were naturally mated and washing of uterine horns was performed at 7 days post-breeding. After collecting the embryos, cloprostenol was injected to donors to induce luteolisis and reinitiate a new follicular wave. Four washes were performed with intervals of 15 days. The transfer of fresh embryos was done on day 7 (group 1) or 6 (group 2) after the induction of ovulation with buserelin to the recipients. The results showed that 58.3% of embryos were collected. Among them, 57.2, 23.8 and 19.0% were of grade 1, 2 and 3 respectively. None embryos were of grade 4. Fertility on day 21 after the transfer of embryos was 33.3 and 11.1% for groups 1 and 2 respectively (mean: 20%), without recording embryo mortality in controls at 90 and 150 days post-transfer.El presente estudio tuvo como objetivo evaluar la eficiencia de recuperación de embriones de ovulación simple, la calidad de los embriones y la fertilidad de la transferencia embrionaria interespecie. Se utilizaron 9 alpacas donadoras y 40 llamas receptoras. Se les hizo el seguimiento ecográfico para determinar la presencia de folículos ovulatorios. Las donadoras fueron cubiertas mediante monta natural y el lavado de los cuernos uterinos se realizó a los 7 días de la cópula. Luego del lavado uterino se aplicó cloprostenol a las donadoras para inducir luteólisis y reiniciar una nueva onda folicular. Se realizaron cuatro lavados con un intervalo de 15 días entre lavados. Las transferencias de los embriones se hicieron en el día 7 (grupo I) y 6 (grupo II) pos-inducción de la ovulación con buserelina a las receptoras. Se recolectó el 58.3% de los embriones. El 57.2, 23.8 y 19.0% de los embriones fueron de grado 1, 2 y 3, respectivamente. No hubo embriones de grado 4. La fertilidad a los 21 días de la transferencia fue de 33.3 y 11.1% para los grupos 1 y 2, respectivamente (promedio: 20.0%), sin registros de mortalidad embrionaria en los controles a los 90 y 150 días de la transferencia.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2016-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1146410.15381/rivep.v27i1.11464Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 1 (2016); 64-69Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 1 (2016); 64-691682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11464/10415Derechos de autor 2016 Joel Pacheco, Víctor Vélez, Danilo Pezohttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/114642016-06-24T10:45:11Z
dc.title.none.fl_str_mv Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
Evaluación de la Eficiencia de la Transferencia de Embriones Interespecie entre Alpacas y Llamas Obtenidos por Ovulación Simple
title Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
spellingShingle Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
Pacheco, Joel
embryos
interspecies transfer
simple ovulation
embriones
transferencia interespecie
ovulación simple
title_short Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
title_full Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
title_fullStr Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
title_full_unstemmed Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
title_sort Evaluation of the efficiency of interspecies embryo transfer between alpacas and llamas obtained through single ovulation
dc.creator.none.fl_str_mv Pacheco, Joel
Vélez, Víctor
Pezo, Danilo
author Pacheco, Joel
author_facet Pacheco, Joel
Vélez, Víctor
Pezo, Danilo
author_role author
author2 Vélez, Víctor
Pezo, Danilo
author2_role author
author
dc.subject.none.fl_str_mv embryos
interspecies transfer
simple ovulation
embriones
transferencia interespecie
ovulación simple
topic embryos
interspecies transfer
simple ovulation
embriones
transferencia interespecie
ovulación simple
description This study aimed to evaluate the efficiency of embryo recovery in single ovulation, the quality of the embryos and the interspecies fertility through embryo transfer. Nine donor alpacas and 40 recipient llamas were used. Ultrasonography of the ovaries was done to determine the presence of an ovulatory follicle. Donors were naturally mated and washing of uterine horns was performed at 7 days post-breeding. After collecting the embryos, cloprostenol was injected to donors to induce luteolisis and reinitiate a new follicular wave. Four washes were performed with intervals of 15 days. The transfer of fresh embryos was done on day 7 (group 1) or 6 (group 2) after the induction of ovulation with buserelin to the recipients. The results showed that 58.3% of embryos were collected. Among them, 57.2, 23.8 and 19.0% were of grade 1, 2 and 3 respectively. None embryos were of grade 4. Fertility on day 21 after the transfer of embryos was 33.3 and 11.1% for groups 1 and 2 respectively (mean: 20%), without recording embryo mortality in controls at 90 and 150 days post-transfer.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11464
10.15381/rivep.v27i1.11464
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11464
identifier_str_mv 10.15381/rivep.v27i1.11464
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11464/10415
dc.rights.none.fl_str_mv Derechos de autor 2016 Joel Pacheco, Víctor Vélez, Danilo Pezo
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2016 Joel Pacheco, Víctor Vélez, Danilo Pezo
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 1 (2016); 64-69
Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 1 (2016); 64-69
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.958958
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