Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR
Descripción del Articulo
The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, L...
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2017 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/13288 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13288 |
| Nivel de acceso: | acceso abierto |
| Materia: | guinea pigs females at first parturition Salmonella multiplex PCR cuyes reproductoras primerizas PCR múltiple |
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Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCRIdentificación Molecular de Salmonella Typhimurium en Cuyes al Primer Parto mediante la Técnica de PCR MúltipleChero O., AnaRosadio A., RaúlMarcelo M., GeraldineDíaz O., GerardoJiménez A., RonaldCastro G., YennyMaturrano H., Leninguinea pigsfemales at first parturitionSalmonellamultiplex PCRcuyesreproductoras primerizasSalmonellaPCR múltipleThe aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding guinea pigs at first parturition in the commercial farm.El estudio tuvo por objetivo identificar el serotipo de los aislados sospechosos de Salmonella spp de cuyes reproductoras en muestras recolectadas dentro de la semana del primer parto con el fin de detectar animales portadores de la bacteria. Los cuyes se encontraban clínicamente sanos y provenían de un criadero comercial de la zona de Pachacamac, Lima, Perú, sin registro de brote de salmonelosis desde hace cuatro años. Se evaluaron 272 muestras pareadas de hisopados rectales y vaginales usando protocolos microbiológicos estandarizados. Las cepas aisladas fueron evaluadas mediante pruebas bioquímicas y se procedió a la extracción de ADN de los aislados sospechosos de Salmonella spp. Posteriormente, estas muestras fueron analizadas mediante la técnica de PCR múltiple para detectar la presencia de los genes invA, prot6E y fliC específicos de Salmonella spp, Salmonella Enteritidis y Salmonella Typhimurium, respectivamente. Ocho animales (12 hisopados) resultaron positivos a Salmonella spp. Los 12 aislados amplificaron al gen invA (Salmonella spp), y de estos, 10 amplificaron para el gen fliC (Salmonella Typhimurium) y ninguno amplificó el gen prot6E (Salmonella Enteritidis). Los resultados confirmaron que Salmonella Typhimurium es el patógeno predominante en cuyes reproductoras al primer parto en esta granja de crianza comercial.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2017-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1328810.15381/rivep.v28i3.13288Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 3 (2017); 679-686Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 3 (2017); 679-6861682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13288/12265Derechos de autor 2017 Ana Chero O., Raúl Rosadio A., Geraldine Marcelo M., Gerardo Díaz O., Ronald Jiménez A., Yenny Castro G., Lenin Maturrano H.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/132882017-12-19T16:57:14Z |
| dc.title.none.fl_str_mv |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR Identificación Molecular de Salmonella Typhimurium en Cuyes al Primer Parto mediante la Técnica de PCR Múltiple |
| title |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR |
| spellingShingle |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR Chero O., Ana guinea pigs females at first parturition Salmonella multiplex PCR cuyes reproductoras primerizas Salmonella PCR múltiple |
| title_short |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR |
| title_full |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR |
| title_fullStr |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR |
| title_full_unstemmed |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR |
| title_sort |
Molecular identification of Salmonella Typhimurium and Enteritidis in guinea pigs at first parturition by multiplex PCR |
| dc.creator.none.fl_str_mv |
Chero O., Ana Rosadio A., Raúl Marcelo M., Geraldine Díaz O., Gerardo Jiménez A., Ronald Castro G., Yenny Maturrano H., Lenin |
| author |
Chero O., Ana |
| author_facet |
Chero O., Ana Rosadio A., Raúl Marcelo M., Geraldine Díaz O., Gerardo Jiménez A., Ronald Castro G., Yenny Maturrano H., Lenin |
| author_role |
author |
| author2 |
Rosadio A., Raúl Marcelo M., Geraldine Díaz O., Gerardo Jiménez A., Ronald Castro G., Yenny Maturrano H., Lenin |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
guinea pigs females at first parturition Salmonella multiplex PCR cuyes reproductoras primerizas Salmonella PCR múltiple |
| topic |
guinea pigs females at first parturition Salmonella multiplex PCR cuyes reproductoras primerizas Salmonella PCR múltiple |
| description |
The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding guinea pigs at first parturition in the commercial farm. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-10-11 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13288 10.15381/rivep.v28i3.13288 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13288 |
| identifier_str_mv |
10.15381/rivep.v28i3.13288 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13288/12265 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 3 (2017); 679-686 Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 3 (2017); 679-686 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
| repository.name.fl_str_mv |
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| repository.mail.fl_str_mv |
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1795238227765886976 |
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13.949868 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
