Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru

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The aim of this study was to identify, serotype and determine the sensitivity profile of Salmonella enterica strains isolated from red-eared slider turtles (Trachemys sp) from two breeding centres in Lima, Peru, through cloacal swabs. The samples were isolated using tetrathionate enrichment broth, s...

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Detalles Bibliográficos
Autores: Meza R., Dante, Morales-Cauti, Siever
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/19022
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19022
Nivel de acceso:acceso abierto
Materia:Salmonella enterica
Trachemys sp
serotyping
Infantis
Saintpaul
serotipifación
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spelling Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, PeruIdentificación, serotipificación y determinación del perfil de sensibilidad de Salmonella enterica aisladas de cloacas de tortugas de orejas rojas (Trachemys sp) en cautiverio, PerúMeza R., DanteMorales-Cauti, SieverSalmonella entericaTrachemys spserotypingInfantisSaintpaulSalmonella entericaTrachemys spserotipifaciónInfantisSaintpaulThe aim of this study was to identify, serotype and determine the sensitivity profile of Salmonella enterica strains isolated from red-eared slider turtles (Trachemys sp) from two breeding centres in Lima, Peru, through cloacal swabs. The samples were isolated using tetrathionate enrichment broth, specific media such as Salmonella-Shigella agar and xylose lysine deoxycholate agar. The identification was made through biochemical tests. Serotyping through the recognition of somatic (O) and flagellar (H) antigens of the Kauffmann-White system, and the determination of sensitivity using the Kirby Bauer diffusion disk test. The results showed that 6.1% (4/66) of Salmonella enterica isolates were obtained, identifying the serotypes Salmonella Saintpaul and Salmonella Infantis. Antibacterial sensitivity tests indicated differences between strains, showing 100% sensitivity against nalidixic acid, norfloxacin and enrofloxacin and less for streptomycin, sulfamethoxazole + trimethoprim, chloramphenicol and amoxicillin + clavulanic acid. The results show the potential risk to public health involved in raising these turtles as potential pets.El presente estudio tuvo por objetivo identificar, serotipificar y determinar el perfil de sensibilidad de cepas de Salmonella enterica aisladas de tortugas de orejas rojas (Trachemys sp) provenientes de dos centros de crianza en Lima, Perú, a través de hisopados cloacales. Las muestras se aislaron por medio del caldo de enriquecimiento tetrationato, medios específicos como agar Salmonella-Shigella y agar xilosa lisina desoxicolato. La identificación se hizo mediante pruebas bioquímicas. La serotipificación a través del reconocimiento de antigenos somáticos (O) y flagelares (H) del sistema Kauffmann-White, y la determinación de la sensibilidad por medio de la prueba de disco difusión Kirby Bauer. Se obtuvo un 6.1% (4/66) de aislados de Salmonella enterica, identificándose los serotipos Salmonella Saintpaul y Salmonella Infantis. Las pruebas de sensibilidad a antibacterianos indicaron diferencias entre cepas, mostrando 100% de sensibilidad frente al ácido nalidíxico, norfloxacína y enrofloxacina y menor a estreptomicina, sulfametoxasol + trimetropin, cloranfenicol y amoxicilina + ácido clavulánico. Los resultados muestran el potencial riesgo para la salud pública que implica la crianza de estas tortugas como potenciales mascotas.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-11-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1902210.15381/rivep.v31i4.19022Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19022Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e190221682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19022/16052Derechos de autor 2020 Dante Meza R., Siever Morales-Cautihttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/190222020-11-26T23:08:49Z
dc.title.none.fl_str_mv Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
Identificación, serotipificación y determinación del perfil de sensibilidad de Salmonella enterica aisladas de cloacas de tortugas de orejas rojas (Trachemys sp) en cautiverio, Perú
title Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
spellingShingle Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
Meza R., Dante
Salmonella enterica
Trachemys sp
serotyping
Infantis
Saintpaul
Salmonella enterica
Trachemys sp
serotipifación
Infantis
Saintpaul
title_short Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
title_full Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
title_fullStr Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
title_full_unstemmed Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
title_sort Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
dc.creator.none.fl_str_mv Meza R., Dante
Morales-Cauti, Siever
author Meza R., Dante
author_facet Meza R., Dante
Morales-Cauti, Siever
author_role author
author2 Morales-Cauti, Siever
author2_role author
dc.subject.none.fl_str_mv Salmonella enterica
Trachemys sp
serotyping
Infantis
Saintpaul
Salmonella enterica
Trachemys sp
serotipifación
Infantis
Saintpaul
topic Salmonella enterica
Trachemys sp
serotyping
Infantis
Saintpaul
Salmonella enterica
Trachemys sp
serotipifación
Infantis
Saintpaul
description The aim of this study was to identify, serotype and determine the sensitivity profile of Salmonella enterica strains isolated from red-eared slider turtles (Trachemys sp) from two breeding centres in Lima, Peru, through cloacal swabs. The samples were isolated using tetrathionate enrichment broth, specific media such as Salmonella-Shigella agar and xylose lysine deoxycholate agar. The identification was made through biochemical tests. Serotyping through the recognition of somatic (O) and flagellar (H) antigens of the Kauffmann-White system, and the determination of sensitivity using the Kirby Bauer diffusion disk test. The results showed that 6.1% (4/66) of Salmonella enterica isolates were obtained, identifying the serotypes Salmonella Saintpaul and Salmonella Infantis. Antibacterial sensitivity tests indicated differences between strains, showing 100% sensitivity against nalidixic acid, norfloxacin and enrofloxacin and less for streptomycin, sulfamethoxazole + trimethoprim, chloramphenicol and amoxicillin + clavulanic acid. The results show the potential risk to public health involved in raising these turtles as potential pets.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19022
10.15381/rivep.v31i4.19022
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19022
identifier_str_mv 10.15381/rivep.v31i4.19022
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19022/16052
dc.rights.none.fl_str_mv Derechos de autor 2020 Dante Meza R., Siever Morales-Cauti
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Dante Meza R., Siever Morales-Cauti
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19022
Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e19022
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.892927
Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru
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