SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES
Descripción del Articulo
Determination of sex in wild birds is crucial in developing conservation plans for threatened species. The absence of sexual dimorphism in birds makes impossible to determine sex based on physical characteristics and sexing has traditionally depended upon surgical methods which are highly stressful...
| Autores: | , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2008 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/1183 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1183 |
| Nivel de acceso: | acceso abierto |
| Materia: | Gen CHD cebadores P2 y P8 guacamayo sexo CHD gene P2 and P8 primers macaw sex |
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SEX DETERMINATION BY ADN IN FIVE MACAW SPECIESDETERMINACIÓN DEL SEXO POR ADN EN CINCO ESPECIES DE GUACAMAYOSLiza R., JacquelineMaturrano H., LeninRosadio A., RaúlGen CHDcebadores P2 y P8guacamayosexoCHD geneP2 and P8 primersmacawsexDetermination of sex in wild birds is crucial in developing conservation plans for threatened species. The absence of sexual dimorphism in birds makes impossible to determine sex based on physical characteristics and sexing has traditionally depended upon surgical methods which are highly stressful for the animals. The present study had the purpose of developing of a noninvasive DNA test for sex determination in macaws (guacamayos). Blood samples from five species (Ara ararauna, Ara chloroptera, Ara macao, Ara militaris y Propyrrhura couloni) were studied. DNA was extracted and specific primers were used to amplify both exons and introns of the chd (chromo-helicase- DNA-binding) gene located on the sex chromosomes of all birds. Females are identified by chd-ZW on the W chromosome and males by chd-ZZ on the Z chromosome. The amplification was carried out by the optimization of the conditions and the PCR cycles. Two fragments of 300-400 bp were detected in female birds and one in males. Sex determined by conventional methods in 31 birds agreed with DNA results. The molecular test was also used to sex 28 macaws of unknown sex, including 11 Propyrrhura couloni.La determinación del sexo en aves de especies silvestres es de vital importancia para tomar medidas de conservación. En especies donde no existe dimorfismo sexual, es necesario contar con una prueba de sexaje alternativo a los métodos quirúrgicos. En este sentido, con la finalidad de estandarizar una prueba para la determinación del sexo en guacamayos, se desarrolló y evaluó una prueba molecular que consistió en el análisis de ADN mediante la Reacción en Cadena de la Polimerasa (PCR). Para ello, se procedió a la extracción de ADN a partir de muestras de sangre cinco especies de guacamayos (Ara ararauna, Ara chloroptera, Ara macao, Ara militaris y Propyrrhura couloni). A través de la técnica de PCR y el uso de primers específicos, se amplificó regiones conservadas (exones) y secuencias de regiones no conservadas (intrones) del gen chd (Chromodomainhelicase- DNA-binding protein), presentes en ambos cromosomas sexuales (Z y W), que permiten diferenciar hembras (chd-ZW) de machos (chd-ZZ). Para la amplificación, se optimizaron las condiciones y los ciclos de PCR. Se pudo detectar dos fragmentos entre 300-400 pares de bases en aves hembras y solamente uno en especies machos. Se observó 100% (31/31) de correspondencia entre el sexaje por métodos convencionales y por análisis de ADN. La prueba molecular fue posteriormente utilizada para sexar a 28 guacamayos de sexo desconocido, incluyendo 11 aves de la especie peruana Propyrrhura couloni.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2008-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/118310.15381/rivep.v19i1.1183Revista de Investigaciones Veterinarias del Perú; Vol. 19 No. 1 (2008); 31-36Revista de Investigaciones Veterinarias del Perú; Vol. 19 Núm. 1 (2008); 31-361682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1183/989Derechos de autor 2008 Jacqueline Liza R., Lenin Maturrano H., Raúl Rosadio A.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:revistasinvestigacion.unmsm.edu.pe:article/11832020-03-16T16:14:49Z |
| dc.title.none.fl_str_mv |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES DETERMINACIÓN DEL SEXO POR ADN EN CINCO ESPECIES DE GUACAMAYOS |
| title |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES |
| spellingShingle |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES Liza R., Jacqueline Gen CHD cebadores P2 y P8 guacamayo sexo CHD gene P2 and P8 primers macaw sex |
| title_short |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES |
| title_full |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES |
| title_fullStr |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES |
| title_full_unstemmed |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES |
| title_sort |
SEX DETERMINATION BY ADN IN FIVE MACAW SPECIES |
| dc.creator.none.fl_str_mv |
Liza R., Jacqueline Maturrano H., Lenin Rosadio A., Raúl |
| author |
Liza R., Jacqueline |
| author_facet |
Liza R., Jacqueline Maturrano H., Lenin Rosadio A., Raúl |
| author_role |
author |
| author2 |
Maturrano H., Lenin Rosadio A., Raúl |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Gen CHD cebadores P2 y P8 guacamayo sexo CHD gene P2 and P8 primers macaw sex |
| topic |
Gen CHD cebadores P2 y P8 guacamayo sexo CHD gene P2 and P8 primers macaw sex |
| description |
Determination of sex in wild birds is crucial in developing conservation plans for threatened species. The absence of sexual dimorphism in birds makes impossible to determine sex based on physical characteristics and sexing has traditionally depended upon surgical methods which are highly stressful for the animals. The present study had the purpose of developing of a noninvasive DNA test for sex determination in macaws (guacamayos). Blood samples from five species (Ara ararauna, Ara chloroptera, Ara macao, Ara militaris y Propyrrhura couloni) were studied. DNA was extracted and specific primers were used to amplify both exons and introns of the chd (chromo-helicase- DNA-binding) gene located on the sex chromosomes of all birds. Females are identified by chd-ZW on the W chromosome and males by chd-ZZ on the Z chromosome. The amplification was carried out by the optimization of the conditions and the PCR cycles. Two fragments of 300-400 bp were detected in female birds and one in males. Sex determined by conventional methods in 31 birds agreed with DNA results. The molecular test was also used to sex 28 macaws of unknown sex, including 11 Propyrrhura couloni. |
| publishDate |
2008 |
| dc.date.none.fl_str_mv |
2008-07-15 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1183 10.15381/rivep.v19i1.1183 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1183 |
| identifier_str_mv |
10.15381/rivep.v19i1.1183 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1183/989 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2008 Jacqueline Liza R., Lenin Maturrano H., Raúl Rosadio A. https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2008 Jacqueline Liza R., Lenin Maturrano H., Raúl Rosadio A. https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 19 No. 1 (2008); 31-36 Revista de Investigaciones Veterinarias del Perú; Vol. 19 Núm. 1 (2008); 31-36 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
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1854938936490590208 |
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13.922529 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
