Detection of the PrP gene in Junin sheep

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The aim of this study was to establish the frequency of resistance and susceptible genotypes to scrapie in the Junin sheep breed. For this, 56 blood samples were collected in a sheep farm in the central highlands of Peru. The DNA was extracted and the PCR products were analyzed by enzymatic digestio...

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Detalles Bibliográficos
Autores: Rivera J., Kamilo, Maturrano H., Lenin, Aguilar, Juan Manuel, Rosadio A., Raúl
Formato: artículo
Fecha de Publicación:2015
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/10917
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10917
Nivel de acceso:acceso abierto
Materia:scrapie
Prion gene
Junin sheep
gen Prión
ovino Junín
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spelling Detection of the PrP gene in Junin sheepDetección del Gen PrP de Scrapie en Ovinos JunínRivera J., KamiloMaturrano H., LeninAguilar, Juan ManuelRosadio A., RaúlscrapiePrion geneJunin sheepscrapiegen Priónovino JunínThe aim of this study was to establish the frequency of resistance and susceptible genotypes to scrapie in the Junin sheep breed. For this, 56 blood samples were collected in a sheep farm in the central highlands of Peru. The DNA was extracted and the PCR products were analyzed by enzymatic digestion using restriction endonucleases that recognize the PrP gene polymorphisms. Four of the 5 haplotypes reported for the PrP gene were identified: ARR (48.2%), ARQ (35.7%), AHQ (10.7%) and VRQ (5.4%). In addition, 7 of the 15 reported genotypes for the Prion gene were found: ARR/ARR (17.9%), ARR/AHQ (10.7%), ARR/ARQ (42.9%), ARQ/AHQ (10.7%), ARQ/ARQ (7.1%), ARR/VRQ (7.1%) and ARQ/VRQ (3.6%). Finally, 82.1% of the animals had genotypes resistant to the disease and 17.9% showed genotypes of susceptibility.El objetivo del presente trabajo fue establecer la frecuencia de genotipos de resistencia y susceptibilidad a padecer la enfermedad de scrapie en ovejas Junín. Se analizaron 56 muestras de sangre de ovinos de una unidad de producción privada de la región central del Perú. Se extrajo el ADN y los productos de PCR fueron analizados mediante digestión enzimática con endonucleasas de restricción que reconocen los polimorfismos del gen PrP. Se pudo identificar 4 de los 5 haplotipos reportados para el gen PrP: ARR (48.2%), ARQ (35.7%), AHQ (10.7%) y VRQ (5.4%). Además, se encontró 7 de los 15 genotipos reportados para el gen Prión: ARR/ARR (17.9%), ARR/AHQ (10.7%), ARR/ ARQ (42.9%), ARQ/AHQ (10.7%), ARQ/ARQ (7.1%), ARR/VRQ (7.1%) y ARQ/VRQ (3.6%). El 82.1% de las ovejas presentaron genotipos de resistencia a la enfermedad y el 17.9% presentó genotipos de susceptibilidad.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2015-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1091710.15381/rivep.v26i1.10917Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 1 (2015); 57-65Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 1 (2015); 57-651682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10917/9965Derechos de autor 2015 Kamilo Rivera J., Lenin Maturrano H., Juan Manuel Aguilar, Raúl Rosadio A.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/109172020-03-29T18:41:05Z
dc.title.none.fl_str_mv Detection of the PrP gene in Junin sheep
Detección del Gen PrP de Scrapie en Ovinos Junín
title Detection of the PrP gene in Junin sheep
spellingShingle Detection of the PrP gene in Junin sheep
Rivera J., Kamilo
scrapie
Prion gene
Junin sheep
scrapie
gen Prión
ovino Junín
title_short Detection of the PrP gene in Junin sheep
title_full Detection of the PrP gene in Junin sheep
title_fullStr Detection of the PrP gene in Junin sheep
title_full_unstemmed Detection of the PrP gene in Junin sheep
title_sort Detection of the PrP gene in Junin sheep
dc.creator.none.fl_str_mv Rivera J., Kamilo
Maturrano H., Lenin
Aguilar, Juan Manuel
Rosadio A., Raúl
author Rivera J., Kamilo
author_facet Rivera J., Kamilo
Maturrano H., Lenin
Aguilar, Juan Manuel
Rosadio A., Raúl
author_role author
author2 Maturrano H., Lenin
Aguilar, Juan Manuel
Rosadio A., Raúl
author2_role author
author
author
dc.subject.none.fl_str_mv scrapie
Prion gene
Junin sheep
scrapie
gen Prión
ovino Junín
topic scrapie
Prion gene
Junin sheep
scrapie
gen Prión
ovino Junín
description The aim of this study was to establish the frequency of resistance and susceptible genotypes to scrapie in the Junin sheep breed. For this, 56 blood samples were collected in a sheep farm in the central highlands of Peru. The DNA was extracted and the PCR products were analyzed by enzymatic digestion using restriction endonucleases that recognize the PrP gene polymorphisms. Four of the 5 haplotypes reported for the PrP gene were identified: ARR (48.2%), ARQ (35.7%), AHQ (10.7%) and VRQ (5.4%). In addition, 7 of the 15 reported genotypes for the Prion gene were found: ARR/ARR (17.9%), ARR/AHQ (10.7%), ARR/ARQ (42.9%), ARQ/AHQ (10.7%), ARQ/ARQ (7.1%), ARR/VRQ (7.1%) and ARQ/VRQ (3.6%). Finally, 82.1% of the animals had genotypes resistant to the disease and 17.9% showed genotypes of susceptibility.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10917
10.15381/rivep.v26i1.10917
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10917
identifier_str_mv 10.15381/rivep.v26i1.10917
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10917/9965
dc.rights.none.fl_str_mv Derechos de autor 2015 Kamilo Rivera J., Lenin Maturrano H., Juan Manuel Aguilar, Raúl Rosadio A.
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2015 Kamilo Rivera J., Lenin Maturrano H., Juan Manuel Aguilar, Raúl Rosadio A.
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 1 (2015); 57-65
Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 1 (2015); 57-65
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238225804001280
score 13.887938
Detection of the PrP gene in Junin sheep
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