Optimization and modification of the method for detection of rhamnolipids

• Autores: Tabuchi, Takeshi, Martínez, Diandra, Hospinal, Manuel, Merino, Fernando, Gutiérrez, Susana
• Formato: artículo
• Fecha de Publicación: 2015
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/11359
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/11359
• Nivel de acceso: acceso abierto
• Materia: Bromuro de Cetiltrimetilamonio (CTAB); azul de metileno; ramnolípidos; biosurfactantes; Pseudomonas.; Cetyltrimethylammonium Bromide (CTAB); methylene blue; Pseudomonas; rhamnolipids; biosurfactants.

Use of biosurfactants in bioremediation, facilitates and accelerates microbial degradation of hydrocarbons. CTAB/MB agar method created by Siegmund & Wagner for screening of rhamnolipids (RL) producing strains, has been widely used but has not improved significantly for more than 20 years. To optimize the technique as a quantitative method, CTAB/MB agar plates were made and different variables were tested, like incubation time, cooling, CTAB concentration, methylene blue presence, wells diameter and inocula volume. Furthermore, a new method for RL detection within halos was developed: precipitation of RL with HCl, allows the formation a new halos pattern, easier to observe and to measure. This research reaffirm that this method is not totally suitable for a fine quantitative analysis, because of the difficulty to accurately correlate RL concentration and the area of the halos. RL diffusion does not seem to have a simple behavior and there are a lot of factors that affect RL migration rate.