Standarization of an ELISA test to detect IgE antibodies in patients with cystic echinococcosis and its use in diagnosis and follow-up of patients treated with albendazol: preliminary report

• Autores: Vildózola, Herman, Espinoza, Irma, Roldan, William H.
• Formato: artículo
• Fecha de Publicación: 2012
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/808
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/808
• Nivel de acceso: acceso abierto
• Materia: ELISA IgE; equinococosis quistica; albendazol.; IgE ELISA; cystic echinococcosis; albendazole.

Objectives: To determine best dilutions and concentrations of ELISA test to detect IgE antibody, its sensitivity and specificityand predictive value in patients with cistyc echinococcosis. To analyze the relationship between IgE specific antibody level andalbendazole medical treatment response in a period over one year after completion of albendazole therapy. Design: Quasiexperimentalstudy with control group. Setting: Instituto de Medicina Tropical Daniel A. Carrion, Faculty of Medicine, UNMSM,Lima, Peru. Study material: IgE antibody ELISA test. Interventions: IgE antibody ELISA test standardization with preexperimentalpretest and post test in one group to assess its value in diagnosis and post-treatment of patients with hepatic hydatid cysts treatedwith albendazole. For ELISA test standardization we used serum from five patients with clinical and immunological diagnosis ofcystic echinococcosis; to test sensitivity and specificity sera of 30 apparently healthy subjects were studied; to determine crossreactions16 serum samples from patients with other helminth infections (ascariasis, strongiloidiasis, toxocariasis, trichuriasis,hymenolepiasis cysticercosis and taeniasis) were used. For diagnosis and post-treatment evaluation of cystic echinocococosisserum from 17 patients was studied. Main outcome measures: Standarized ELISA test sensitivity and specificity to detect IgEantibodies. Results: Sensitivity and specificity of standardized ELISA test to detect IgE antibody were respectively 95.6% and 100%.Patients with hepatic hydatid cyst considered cured uniformly showed lower levels of IgE antibody even to negativization. There wasincrease of IgE levels in patients who had recurrence of hepatic cystic disease. Conclusions: This study found excellent sensitivity andspecificity of ELISA test for cystic echinococcosis IgE antibodies. Healing of cysts coincided with progressive decrease in IgE levelsthat could reach negativization after one year post-treatment and was useful to detect recurrence. Persistence of IgE high levels inpatients treated medically was indicative of no response to treatment. As sample size was not large, it seems necessary to continueinvestigation to confirm these results.