Genotoxic analysis of water samples from the Ramis River (Puno Department, Peru) using erythrocytes from the peripheral blood of Zebrafish (Danio rerio)

• Autores: Scotto, Carlos, Álvarez, Javier, Llanos, Carlos, Leyva, Omar, Sotomayor, Sául, Chávez, Karol, Justo, Eber, Casso, Oliver, Larico, César, Mayta, Wilmar, Sanga, Wilson
• Formato: artículo
• Fecha de Publicación: 2013
• Institución: Universidad Nacional Federico Villarreal
• Repositorio: Revistas - Universidad Nacional Federico Villarreal
• Lenguaje: español
• OAI Identifier: oai:ojs2.revistas.unfv.edu.pe:article/6
• Enlace del recurso: https://revistas.unfv.edu.pe/RCV/article/view/6
• Nivel de acceso: acceso abierto
• Materia: Danio rerio; eritrocitos; test de micronúcleos; genotoxicidad; ciclosfosfamida; relave

Mine tailings release various genotoxic compounds into the environment. The surrounding river basins are the most affected, which is why it is important to determine the quality of their waters. The Micronucleus (MN) test quantifies the genotoxic damage produced in the erythrocytes of Zebrafish (Danio rerio) by the action of genotoxic compounds present in the waters of contaminated rivers. Therefore, the present investigation analyzed five points in the Ramis River basin (department of Puno, Peru) using the MN test and Zebrafish as a bioindicator, which were evaluated at 24, 48 and 72 hours of exposure to three different volumes (1X, 1/2X and 1/4X) for each sampled point. Among the most notable results, after 24 hours of exposure, we have the sample taken from the Lunar de Oro lagoon (M5) that was fatal (100%) at 1X; while the sample taken from Pampilla (M4) presented up to 23% MN at 1/2X; and the sample from the Taraco River (M1) presented 12% MN. The analysis after 48 and 72 hours of exposure showed the presence of a maximum of 5% of MN for all samples. With this we can conclude that the MN test serves as a rapid and effective genotoxicity test to determine the water quality of rivers contaminated by mining tailings.