SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS

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“Kañiwa" (Chenopodium pallidicaule Aellen) is a very nutritious Andean grain characterized by its high protein content and good amino acid profile. The diversity of this species has been characterized by agro-morphological and genomic studies, which are not completely efficient to discriminate...

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Detalles Bibliográficos
Autores: Ríos, Carlos, Espichán, Fabio, Candy Ruiz, Candy Ruiz, Rojas, Rosario
Formato: artículo
Fecha de Publicación:2020
Institución:Sociedad Química del Perú
Repositorio:Revista de la Sociedad Química del Perú
Lenguaje:español
OAI Identifier:oai:rsqp.revistas.sqperu.org.pe:article/266
Enlace del recurso:http://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/266
Nivel de acceso:acceso abierto
Materia:Chenopodium pallidicaule
kañiwa
metabolomics
UHPLC-MSMS
metabolómica
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spelling SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICSBÚSQUEDA DE BIOMARCADORES PARA LA DISCRIMINACIÓN DE LAS VARIEDADES DE KAÑIWA (Chenopodium pallidicaule Aellen) “CHILLIWA” Y “RAMIS” POR MEDIO DE METABOLÓMICA NON-TARGETEDRíos, CarlosEspichán, FabioCandy Ruiz, Candy RuizRojas, RosarioChenopodium pallidicaulekañiwametabolomicsUHPLC-MSMSkañiwaChenopodium pallidicaulemetabolómicaUHPLC-MSMS“Kañiwa" (Chenopodium pallidicaule Aellen) is a very nutritious Andean grain characterized by its high protein content and good amino acid profile. The diversity of this species has been characterized by agro-morphological and genomic studies, which are not completely efficient to discriminate between accessions and varieties. Metabolomics is currently used as an additional tool for species delimitation. In this sense, in the present work a nontargeted metabolomic analysis of two varieties of kañiwa (“Ramis” and “Chilliwa”) from the department of Puno, Peru was carried out. For the analysis, ultra-high performance liquid chromatography coupled to high resolution mass spectrometry with Orbitrap analyzer (UHPLC-HRMS) was used. The MSDIAL and MSFINDER software were used to identify the metabolites. The chemical profiles obtained were summarized in a single table for statistical analysis with the SIMCA P software. The OPLS-DA statistical model allowed the classification of both varieties of kañiwa and the S-plot graph showed the main putative biomarkers for discrimination of both varieties. The metabolite delfinidine-O-(6''-O-α- ramnopyranosyl-β-glucopyranoside) was identified as one of the main biomarkers for kañiwa “Ramis”.La “kañiwa” (Chenopodium pallidicaule Aellen) es un grano andino muy nutritivo caracterizado por su alto contenido de proteínas y buen perfil de aminoácidos. La diversidad de esta especie viene siendo caracterizada por estudios agro-morfológicos y genómicos, los cuales no son completamente eficientes para discriminar entre las accesiones y variedades. Actualmente se utiliza la metabolómica como una herramienta adicional para la delimitación de especies. En tal sentido, en el presente trabajo se llevó a cabo el análisis metabolómico “non-targeted” de dos variedades de kañiwa (“Ramis” y “Chilliwa”) provenientes del departamento de Puno, Perú. Para el análisis se utilizó la cromatografía líquida de ultra-alta performance acoplada a la espectrometría de masas de alta resolución con analizador Orbitrap (UHPLC-HRMS). Para la identificación de los metabolitos se utilizaron los softwares MSDIAL y MSFINDER. Los perfiles químicos obtenidos fueron resumidos en una sola tabla para su análisis estadístico con el software SIMCA P. El modelo estadístico OPLS-DA permitió la clasificación de ambas variedades de kañiwa y el gráfico S-plot puso en evidencia los principales biomarcadores putativos para la discriminación de ambas variedades. El metabolito delfinidina-O-(6''-O-α-ramnopiranosil-β-glucopiranósido) fue identificado como uno de los principales biomarcadores para la kañiwa “Ramis”.Sociedad Química del Perú2020-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexttextoapplication/pdfhttp://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/26610.37761/rsqp.v86i1.266Revista de la Sociedad Química del Perú; Vol. 86 Núm. 1 (2020): Revista de la Sociedad Química del Perú; 13 - 23Journal of the Chemical Society of Peru; Vol. 86 No. 1 (2020): Journal of Sociedad Química del Perú; 13 - 232309-87401810-634X10.37761/rsqp.v86i1reponame:Revista de la Sociedad Química del Perúinstname:Sociedad Química del Perúinstacron:SQPspahttp://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/266/23420202020Derechos de autor 2020 Sociedad Química del Perúhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:rsqp.revistas.sqperu.org.pe:article/2662020-07-31T15:48:16Z
dc.title.none.fl_str_mv SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
BÚSQUEDA DE BIOMARCADORES PARA LA DISCRIMINACIÓN DE LAS VARIEDADES DE KAÑIWA (Chenopodium pallidicaule Aellen) “CHILLIWA” Y “RAMIS” POR MEDIO DE METABOLÓMICA NON-TARGETED
title SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
spellingShingle SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
Ríos, Carlos
Chenopodium pallidicaule
kañiwa
metabolomics
UHPLC-MSMS
kañiwa
Chenopodium pallidicaule
metabolómica
UHPLC-MSMS
title_short SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
title_full SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
title_fullStr SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
title_full_unstemmed SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
title_sort SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
dc.creator.none.fl_str_mv Ríos, Carlos
Espichán, Fabio
Candy Ruiz, Candy Ruiz
Rojas, Rosario
author Ríos, Carlos
author_facet Ríos, Carlos
Espichán, Fabio
Candy Ruiz, Candy Ruiz
Rojas, Rosario
author_role author
author2 Espichán, Fabio
Candy Ruiz, Candy Ruiz
Rojas, Rosario
author2_role author
author
author
dc.subject.none.fl_str_mv Chenopodium pallidicaule
kañiwa
metabolomics
UHPLC-MSMS
kañiwa
Chenopodium pallidicaule
metabolómica
UHPLC-MSMS
topic Chenopodium pallidicaule
kañiwa
metabolomics
UHPLC-MSMS
kañiwa
Chenopodium pallidicaule
metabolómica
UHPLC-MSMS
description “Kañiwa" (Chenopodium pallidicaule Aellen) is a very nutritious Andean grain characterized by its high protein content and good amino acid profile. The diversity of this species has been characterized by agro-morphological and genomic studies, which are not completely efficient to discriminate between accessions and varieties. Metabolomics is currently used as an additional tool for species delimitation. In this sense, in the present work a nontargeted metabolomic analysis of two varieties of kañiwa (“Ramis” and “Chilliwa”) from the department of Puno, Peru was carried out. For the analysis, ultra-high performance liquid chromatography coupled to high resolution mass spectrometry with Orbitrap analyzer (UHPLC-HRMS) was used. The MSDIAL and MSFINDER software were used to identify the metabolites. The chemical profiles obtained were summarized in a single table for statistical analysis with the SIMCA P software. The OPLS-DA statistical model allowed the classification of both varieties of kañiwa and the S-plot graph showed the main putative biomarkers for discrimination of both varieties. The metabolite delfinidine-O-(6''-O-α- ramnopyranosyl-β-glucopyranoside) was identified as one of the main biomarkers for kañiwa “Ramis”.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Text
texto
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/266
10.37761/rsqp.v86i1.266
url http://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/266
identifier_str_mv 10.37761/rsqp.v86i1.266
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/266/234
dc.rights.none.fl_str_mv Derechos de autor 2020 Sociedad Química del Perú
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Sociedad Química del Perú
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv 2020
2020
dc.publisher.none.fl_str_mv Sociedad Química del Perú
publisher.none.fl_str_mv Sociedad Química del Perú
dc.source.none.fl_str_mv Revista de la Sociedad Química del Perú; Vol. 86 Núm. 1 (2020): Revista de la Sociedad Química del Perú; 13 - 23
Journal of the Chemical Society of Peru; Vol. 86 No. 1 (2020): Journal of Sociedad Química del Perú; 13 - 23
2309-8740
1810-634X
10.37761/rsqp.v86i1
reponame:Revista de la Sociedad Química del Perú
instname:Sociedad Química del Perú
instacron:SQP
instname_str Sociedad Química del Perú
instacron_str SQP
institution SQP
reponame_str Revista de la Sociedad Química del Perú
collection Revista de la Sociedad Química del Perú
repository.name.fl_str_mv
repository.mail.fl_str_mv
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SEARCH FOR BIOMARKERS FOR THE DISCRIMINATION OF THE “CHILLIWA” AND “RAMIS” VARIETIES OF KAÑIWA TROUGH NON-TARGETED METABOLOMICS
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