EFFECT OF DIFFERENT CONCENTRATIONS OF BENZYLAMINOPURINE ON THE MICROPROPAGATION OF TWO VARIETIES OF NATIVE CHILI (Capsicum chinense Jacq.) IN PUCALLPA

Descripción del Articulo

The effect of different concentrations of Benzylaminopurine (BAP) on the micropropagation of native chili (sweet chili and pucunucho chili) was verified. Seeds were disinfected with 2 % sodium hypochlorite for 30 minutes, rinsed with sterile distilled water and sown in culture medium with salts and...

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Detalles Bibliográficos
Autores: VILLEGAS-PANDURO, Pablo Pedro, ALARCÓN-CASTILLO, Teresa, RODRÍGUEZ-ORÉ, Melvin Elvis
Formato: artículo
Fecha de Publicación:2021
Institución:Instituto de investigaciones de la Amazonía Peruana
Repositorio:Folia Amazónica
Lenguaje:español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/541
Enlace del recurso:https://revistas.iiap.gob.pe/index.php/foliaamazonica/article/view/541
Nivel de acceso:acceso abierto
Materia:citoquininas
propagación clonal
hormonas vegetales
explantes
chiles
cytokinins
clonal propagation
plant hormones
explants
chili peppers
Descripción
Sumario:The effect of different concentrations of Benzylaminopurine (BAP) on the micropropagation of native chili (sweet chili and pucunucho chili) was verified. Seeds were disinfected with 2 % sodium hypochlorite for 30 minutes, rinsed with sterile distilled water and sown in culture medium with salts and vitamins from Murashige & Skoog, supplemented with 30 g L-1 of sucrose, 11 g L-1 of agar and without growth regulators. After 45 days of in vitro development, the seedlings were transferred to Murashige & Skoog medium, adding different concentrations of BAP (0 ppm, 0.5 ppm, 1 ppm and 1.5 ppm). It is concluded that the sanitization of the seeds of sweet chili and pucunucho chili with 2 % sodium hypochlorite for 30 minutes generated 100 % of healthy seeds. The concentrations of BAP studied were effective for the increase in the length of stems, number of roots, number of leaves, nodes and callus formation in sweet chili seedlings. On the other hand, for the seedlings of pucunucho chili, the increase in the number of leaves, nodes and roots occurred in the absence of concentrations of BAP, except for the generation of callus tissue.
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