Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus
Descripción del Articulo
The present investigation was done to evaluate lethal dose fifty (DL50) and effective dose average (DE50) of the antiinflammatory effect of a methanolic extract of the leaves of Pelargonium robertianum L."Geranio". To evaluate the DL50, 36 mice were used, divided in groups of 6 animals tha...
| Autor: | |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2022 |
| Institución: | Universidad de San Martín de Porres |
| Repositorio: | Horizonte médico |
| Lenguaje: | español |
| OAI Identifier: | oai:horizontemedico.usmp.edu.pe:article/2033 |
| Enlace del recurso: | https://www.horizontemedico.usmp.edu.pe/index.php/horizontemed/article/view/2033 |
| Nivel de acceso: | acceso abierto |
| Materia: | Pelargonium robertianum L Gera-nium Diclofenac Inflammation Toxicity DL50 DE50 Phytochemical Screening HPLC RMNC13 RMNHI IR Alcaloid Flavonoid Rutin Quercetin Antiinflamatorio Geranio Diclofenaco Inflamación Toxicidad Screening Fitoquímico HPLCRMNC13 Alcaloide Flavonoide Rutina Quercetina |
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Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus Caracterización química y efecto antiinflamatorio del extracto metanólico de las hojas de pelargonium robertianum L, «Geranio», en mus musculus |
| title |
Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus |
| spellingShingle |
Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus Ibáñez V., Lucy A. Pelargonium robertianum L Gera-nium Diclofenac Inflammation Toxicity DL50 DE50 Phytochemical Screening HPLC RMNC13 RMNHI IR Alcaloid Flavonoid Rutin Quercetin Antiinflamatorio Pelargonium robertianum L Geranio Diclofenaco Inflamación Toxicidad DL50 DE50 Screening Fitoquímico HPLCRMNC13 RMNHI IR Alcaloide Flavonoide Rutina Quercetina |
| title_short |
Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus |
| title_full |
Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus |
| title_fullStr |
Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus |
| title_full_unstemmed |
Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus |
| title_sort |
Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculus |
| dc.creator.none.fl_str_mv |
Ibáñez V., Lucy A. |
| author |
Ibáñez V., Lucy A. |
| author_facet |
Ibáñez V., Lucy A. |
| author_role |
author |
| dc.subject.none.fl_str_mv |
Pelargonium robertianum L Gera-nium Diclofenac Inflammation Toxicity DL50 DE50 Phytochemical Screening HPLC RMNC13 RMNHI IR Alcaloid Flavonoid Rutin Quercetin Antiinflamatorio Pelargonium robertianum L Geranio Diclofenaco Inflamación Toxicidad DL50 DE50 Screening Fitoquímico HPLCRMNC13 RMNHI IR Alcaloide Flavonoide Rutina Quercetina |
| topic |
Pelargonium robertianum L Gera-nium Diclofenac Inflammation Toxicity DL50 DE50 Phytochemical Screening HPLC RMNC13 RMNHI IR Alcaloid Flavonoid Rutin Quercetin Antiinflamatorio Pelargonium robertianum L Geranio Diclofenaco Inflamación Toxicidad DL50 DE50 Screening Fitoquímico HPLCRMNC13 RMNHI IR Alcaloide Flavonoide Rutina Quercetina |
| description |
The present investigation was done to evaluate lethal dose fifty (DL50) and effective dose average (DE50) of the antiinflammatory effect of a methanolic extract of the leaves of Pelargonium robertianum L."Geranio". To evaluate the DL50, 36 mice were used, divided in groups of 6 animals that received different doses of the methanolic extract by oral route using intragastric cannulae. Animals were observed during the first 24, 48 and 72 hours. The value of the DL50 was of 8,717.02 mg/kg by the Lichfield and Wilcoxon method. DE50 following Winter's Model (1970) and Sughisita et.al. was also performed. We observed that the methanolic extract presented an inhibitory effect of inflammation when compared to that of Diclofenac (62%) obtaining 26, 38 and 57% for the different doses assayed. The Average Effective Dose obtained was of 681,9577 by the method of Probits with ANOVA (p=0.0001,F=5240, R2=0.9979). From the extract with antiinflammatory activity, phenolic compounds and the flavonoids Rutin and Quercetin were isolated, both inhibitors of cyclooxygenase. These compounds were observed by HPLC and TLC respectively and also three alkaloids named GM1, GM2,GM3, were analyzed by means of IR, HPLC, RMNC13, RMNHI. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-08-18 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://www.horizontemedico.usmp.edu.pe/index.php/horizontemed/article/view/2033 10.24265/horizmed.2004.V4n2.09 |
| url |
https://www.horizontemedico.usmp.edu.pe/index.php/horizontemed/article/view/2033 |
| identifier_str_mv |
10.24265/horizmed.2004.V4n2.09 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://www.horizontemedico.usmp.edu.pe/index.php/horizontemed/article/view/2033/1255 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2022 Horizonte Médico (Lima) https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2022 Horizonte Médico (Lima) https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad de San Martín de Porres. Facultad de Medicina Humana |
| publisher.none.fl_str_mv |
Universidad de San Martín de Porres. Facultad de Medicina Humana |
| dc.source.none.fl_str_mv |
Horizonte Médico (Lima); Vol. 4 No. 2 (2004): July - December; 82-86 Horizonte Médico (Lima); Vol. 4 Núm. 2 (2004): Julio - Diciembre; 82-86 Horizonte Médico (Lima); v. 4 n. 2 (2004): Julio - Diciembre; 82-86 2227-3530 1727-558X reponame:Horizonte médico instname:Universidad de San Martín de Porres instacron:USMP |
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Universidad de San Martín de Porres |
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USMP |
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USMP |
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Horizonte médico |
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Horizonte médico |
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1843452337519067136 |
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Chemical characterization and antinflammatory activity of the methanolic extract of Pelargonium robertianum L., "Geranio", in Mus musculusCaracterización química y efecto antiinflamatorio del extracto metanólico de las hojas de pelargonium robertianum L, «Geranio», en mus musculusIbáñez V., Lucy A.Pelargonium robertianum LGera-niumDiclofenacInflammationToxicityDL50DE50Phytochemical ScreeningHPLCRMNC13RMNHIIRAlcaloidFlavonoidRutinQuercetinAntiinflamatorioPelargonium robertianum LGeranioDiclofenaco InflamaciónToxicidadDL50DE50Screening FitoquímicoHPLCRMNC13RMNHIIRAlcaloideFlavonoideRutinaQuercetinaThe present investigation was done to evaluate lethal dose fifty (DL50) and effective dose average (DE50) of the antiinflammatory effect of a methanolic extract of the leaves of Pelargonium robertianum L."Geranio". To evaluate the DL50, 36 mice were used, divided in groups of 6 animals that received different doses of the methanolic extract by oral route using intragastric cannulae. Animals were observed during the first 24, 48 and 72 hours. The value of the DL50 was of 8,717.02 mg/kg by the Lichfield and Wilcoxon method. DE50 following Winter's Model (1970) and Sughisita et.al. was also performed. We observed that the methanolic extract presented an inhibitory effect of inflammation when compared to that of Diclofenac (62%) obtaining 26, 38 and 57% for the different doses assayed. The Average Effective Dose obtained was of 681,9577 by the method of Probits with ANOVA (p=0.0001,F=5240, R2=0.9979). From the extract with antiinflammatory activity, phenolic compounds and the flavonoids Rutin and Quercetin were isolated, both inhibitors of cyclooxygenase. These compounds were observed by HPLC and TLC respectively and also three alkaloids named GM1, GM2,GM3, were analyzed by means of IR, HPLC, RMNC13, RMNHI.La presente investigación se realizó con el objetivo de evaluar la Dosis Letal Media (DL50) y la Dosis Efectiva Media del efecto antiinflamatorio (DE50) del extracto metanólico de las hojas de Pelargonium robertianum L., "Geranio".Para la DL50 se utilizaron 36 ratones en ayuno de 12 horas dividiéndose en grupos de seis animales, administrándose dosis diferentes del extracto metanólico por vía oral mediante cánulas intragástricas, observándose constantemente durante las 24, 48 y 72 horas. El valor de la DL50 fue de 8,717.02 mg/kg de peso de acuerdo al Método de Lichfield y Wilcoxon.Asimismo, la DE50 se realizó, según el Modelo de Winter (1970) y Sughisita et.al. Se observó que el extracto metanólico presentó un efecto inhibidor de la inflamación comparable al presentado por Diclofenaco (62%), obteniéndose el 26, 38 y 57% para las diferentes dosis ensayadas y la Dosis Efectiva Media (DE50) fue de 681.9577 mg/kg de peso mediante el método de los Probits. Al evaluar los datos por el Análisis de Varianza se observó resultados significativos siendo p <0.0001, F=5240 y un R2 de 0.9979. Del extracto con actividad antiinflamatoria se aislaron compuestos fenólicos y los flavonoides: Rutina y Quercetina, estos dos últimos inhibidores de la ciclooxigenasa observados por HPLC y TLC respectivamente, así como 3 alcaloides denominados GMI, GM2 y GM3 los cuales fueron analizados mediante técnicas de IR, HPLC, RMNC13, RMNH1.Universidad de San Martín de Porres. Facultad de Medicina Humana2022-08-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.horizontemedico.usmp.edu.pe/index.php/horizontemed/article/view/203310.24265/horizmed.2004.V4n2.09Horizonte Médico (Lima); Vol. 4 No. 2 (2004): July - December; 82-86Horizonte Médico (Lima); Vol. 4 Núm. 2 (2004): Julio - Diciembre; 82-86Horizonte Médico (Lima); v. 4 n. 2 (2004): Julio - Diciembre; 82-862227-35301727-558Xreponame:Horizonte médicoinstname:Universidad de San Martín de Porresinstacron:USMPspahttps://www.horizontemedico.usmp.edu.pe/index.php/horizontemed/article/view/2033/1255Derechos de autor 2022 Horizonte Médico (Lima)https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:horizontemedico.usmp.edu.pe:article/20332022-08-18T03:51:56Z |
| score |
13.461011 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
