Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia
Descripción del Articulo
280-2010-CONCYTEC-OAJ
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2015 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/1248 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/1248 https://doi.org/10.1007/s10535-015-0540-z |
| Nivel de acceso: | acceso abierto |
| Materia: | L-galactosa deshidrogenasa GDP-D-manosa pirofosforilasa GDP-D-manosa-3′,5′-epimerasa GDP-D-manosa-3′,5′-epimerasa GDP-L-galactosa fosforilasa L-galactono-1-4-lactona deshidrogenasa L-galactosa-1-fosfato fosfatasa https://purl.org/pe-repo/ocde/ford#1.06.10 |
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CONC_cb2e72f6e40eac2565e4e4c29f9a02cb |
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oai:repositorio.concytec.gob.pe:20.500.12390/1248 |
| network_acronym_str |
CONC |
| network_name_str |
CONCYTEC-Institucional |
| repository_id_str |
4689 |
| dc.title.none.fl_str_mv |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| spellingShingle |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia Castro, JC L-galactosa deshidrogenasa GDP-D-manosa pirofosforilasa GDP-D-manosa-3′,5′-epimerasa GDP-D-manosa-3′,5′-epimerasa GDP-L-galactosa fosforilasa L-galactono-1-4-lactona deshidrogenasa L-galactosa-1-fosfato fosfatasa https://purl.org/pe-repo/ocde/ford#1.06.10 |
| title_short |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_full |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_fullStr |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_full_unstemmed |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_sort |
Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| author |
Castro, JC |
| author_facet |
Castro, JC Cobos, M Maddox, JD Iman, SA Egoavil, A Torres, J Gutierrez, F |
| author_role |
author |
| author2 |
Cobos, M Maddox, JD Iman, SA Egoavil, A Torres, J Gutierrez, F |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Castro, JC Cobos, M Maddox, JD Iman, SA Egoavil, A Torres, J Gutierrez, F |
| dc.subject.none.fl_str_mv |
L-galactosa deshidrogenasa |
| topic |
L-galactosa deshidrogenasa GDP-D-manosa pirofosforilasa GDP-D-manosa-3′,5′-epimerasa GDP-D-manosa-3′,5′-epimerasa GDP-L-galactosa fosforilasa L-galactono-1-4-lactona deshidrogenasa L-galactosa-1-fosfato fosfatasa https://purl.org/pe-repo/ocde/ford#1.06.10 |
| dc.subject.es_PE.fl_str_mv |
GDP-D-manosa pirofosforilasa GDP-D-manosa-3′,5′-epimerasa GDP-D-manosa-3′,5′-epimerasa GDP-L-galactosa fosforilasa L-galactono-1-4-lactona deshidrogenasa L-galactosa-1-fosfato fosfatasa |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#1.06.10 |
| description |
280-2010-CONCYTEC-OAJ |
| publishDate |
2015 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2015 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/1248 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1007/s10535-015-0540-z |
| dc.identifier.isi.none.fl_str_mv |
368447400022 |
| url |
https://hdl.handle.net/20.500.12390/1248 https://doi.org/10.1007/s10535-015-0540-z |
| identifier_str_mv |
368447400022 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Biologia Plantarum |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Springer Netherlands |
| publisher.none.fl_str_mv |
Springer Netherlands |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
| instname_str |
Consejo Nacional de Ciencia Tecnología e Innovación |
| instacron_str |
CONCYTEC |
| institution |
CONCYTEC |
| reponame_str |
CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
| repository.name.fl_str_mv |
Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1839175831524474880 |
| spelling |
Publicationrp01900500rp03603600rp03605600rp03606600rp03602600rp01109500rp03604600Castro, JCCobos, MMaddox, JDIman, SAEgoavil, ATorres, JGutierrez, F2024-05-30T23:13:38Z2024-05-30T23:13:38Z2015https://hdl.handle.net/20.500.12390/1248https://doi.org/10.1007/s10535-015-0540-z368447400022280-2010-CONCYTEC-OAJThe aim of this work was to elucidate the molecular and biochemical mechanisms that control L-ascorbic acid (AsA) content variation in Myrciaria dubia. The AsA was quantified by high-performance liquid chromatography, gene expression by real-time quantitative PCR, and enzyme activities by spectrophotometric methods from leaves and immature fruits of two genotypes (Md-60,06 and Md-02,04) with pronounced (about 2 times) differences in the AsA content. In either genotype, the fruit peel had ∼ 1.5 times more AsA than the fruit pulp and ∼ 15.0 times more than the leaf. All tissues examined demonstrated the capability for AsA biosynthesis through the D-mannose/L-galactose pathway because mRNAs of the six key genes [GDP-D-mannose pyrophosphorylase (GMP), GDP-D-mannose-3′,5′-epimerase (GME), GDP-L-galactose phosphorylase (GGP), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH), and L-galactono-1-4-lactone dehydrogenase (GLDH)] and catalytic activities of the corresponding enzymes (GMP, GDH, and GLDH) were detected. The differential expressions of genes and enzyme activities mostly correlated with the respective AsA content. Thus, the expression of several genes of the D-mannose/L-galactose pathway determined the AsA content variation in tissues of M. dubia.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengSpringer NetherlandsBiologia Plantaruminfo:eu-repo/semantics/openAccessL-galactosa deshidrogenasaGDP-D-manosa pirofosforilasa-1GDP-D-manosa-3′,5′-epimerasa-1GDP-D-manosa-3′,5′-epimerasa-1GDP-L-galactosa fosforilasa-1L-galactono-1-4-lactona deshidrogenasa-1L-galactosa-1-fosfato fosfatasa-1https://purl.org/pe-repo/ocde/ford#1.06.10-1Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubiainfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/1248oai:repositorio.concytec.gob.pe:20.500.12390/12482024-05-30 16:02:06.481http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="fc482a4d-9227-49a1-a50a-3ae00954ed24"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia</Title> <PublishedIn> <Publication> <Title>Biologia Plantarum</Title> </Publication> </PublishedIn> <PublicationDate>2015</PublicationDate> <DOI>https://doi.org/10.1007/s10535-015-0540-z</DOI> <ISI-Number>368447400022</ISI-Number> <Authors> <Author> <DisplayName>Castro, JC</DisplayName> <Person id="rp01900" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Cobos, M</DisplayName> <Person id="rp03603" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Maddox, JD</DisplayName> <Person id="rp03605" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Iman, SA</DisplayName> <Person id="rp03606" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Egoavil, A</DisplayName> <Person id="rp03602" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Torres, J</DisplayName> <Person id="rp01109" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Gutierrez, F</DisplayName> <Person id="rp03604" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Springer Netherlands</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>L-galactosa deshidrogenasa</Keyword> <Keyword>GDP-D-manosa pirofosforilasa</Keyword> <Keyword>GDP-D-manosa-3′,5′-epimerasa</Keyword> <Keyword>GDP-D-manosa-3′,5′-epimerasa</Keyword> <Keyword>GDP-L-galactosa fosforilasa</Keyword> <Keyword>L-galactono-1-4-lactona deshidrogenasa</Keyword> <Keyword>L-galactosa-1-fosfato fosfatasa</Keyword> <Abstract>The aim of this work was to elucidate the molecular and biochemical mechanisms that control L-ascorbic acid (AsA) content variation in Myrciaria dubia. The AsA was quantified by high-performance liquid chromatography, gene expression by real-time quantitative PCR, and enzyme activities by spectrophotometric methods from leaves and immature fruits of two genotypes (Md-60,06 and Md-02,04) with pronounced (about 2 times) differences in the AsA content. In either genotype, the fruit peel had ∼ 1.5 times more AsA than the fruit pulp and ∼ 15.0 times more than the leaf. All tissues examined demonstrated the capability for AsA biosynthesis through the D-mannose/L-galactose pathway because mRNAs of the six key genes [GDP-D-mannose pyrophosphorylase (GMP), GDP-D-mannose-3′,5′-epimerase (GME), GDP-L-galactose phosphorylase (GGP), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH), and L-galactono-1-4-lactone dehydrogenase (GLDH)] and catalytic activities of the corresponding enzymes (GMP, GDH, and GLDH) were detected. The differential expressions of genes and enzyme activities mostly correlated with the respective AsA content. Thus, the expression of several genes of the D-mannose/L-galactose pathway determined the AsA content variation in tissues of M. dubia.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
| score |
13.448642 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
