Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes
Descripción del Articulo
This study was supported by Grant PIBA15A106 and B17100101 Universidad Nacional Mayor de San Marcos and National Council of Science Technology and Innovation N° 313-2011-CONCYTEC-OAJ. This article includes results of Martha Valdivia ?octoral Thesis of Sciences with mention of Physiology, Universidad...
| Autores: | , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2019 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/942 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/942 https://doi.org/10.1111/and.13283 |
| Nivel de acceso: | acceso abierto |
| Materia: | SSC Alpaca DBA epididymal sperm mobility molecular markers https://purl.org/pe-repo/ocde/ford#1.06.03 |
| id |
CONC_9f058241c93b3c986e2ce5f9e3cf29ff |
|---|---|
| oai_identifier_str |
oai:repositorio.concytec.gob.pe:20.500.12390/942 |
| network_acronym_str |
CONC |
| network_name_str |
CONCYTEC-Institucional |
| repository_id_str |
4689 |
| dc.title.none.fl_str_mv |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes |
| title |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes |
| spellingShingle |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes Valdivia M. SSC Alpaca DBA epididymal sperm mobility molecular markers https://purl.org/pe-repo/ocde/ford#1.06.03 |
| title_short |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes |
| title_full |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes |
| title_fullStr |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes |
| title_full_unstemmed |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes |
| title_sort |
Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes |
| author |
Valdivia M. |
| author_facet |
Valdivia M. Castañeda-Zegarra S. Lévano G. Lazo J. Reyes J. Bravo Z. Santiani A. Mujica F. Ruíz J. Gonzales G.F. |
| author_role |
author |
| author2 |
Castañeda-Zegarra S. Lévano G. Lazo J. Reyes J. Bravo Z. Santiani A. Mujica F. Ruíz J. Gonzales G.F. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Valdivia M. Castañeda-Zegarra S. Lévano G. Lazo J. Reyes J. Bravo Z. Santiani A. Mujica F. Ruíz J. Gonzales G.F. |
| dc.subject.none.fl_str_mv |
SSC |
| topic |
SSC Alpaca DBA epididymal sperm mobility molecular markers https://purl.org/pe-repo/ocde/ford#1.06.03 |
| dc.subject.es_PE.fl_str_mv |
Alpaca DBA epididymal sperm mobility molecular markers |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#1.06.03 |
| description |
This study was supported by Grant PIBA15A106 and B17100101 Universidad Nacional Mayor de San Marcos and National Council of Science Technology and Innovation N° 313-2011-CONCYTEC-OAJ. This article includes results of Martha Valdivia ?octoral Thesis of Sciences with mention of Physiology, Universidad Peruana Cayetano |
| publishDate |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/942 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1111/and.13283 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-85063990103 |
| url |
https://hdl.handle.net/20.500.12390/942 https://doi.org/10.1111/and.13283 |
| identifier_str_mv |
2-s2.0-85063990103 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Blackwell Publishing Ltd |
| publisher.none.fl_str_mv |
Blackwell Publishing Ltd |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
| instname_str |
Consejo Nacional de Ciencia Tecnología e Innovación |
| instacron_str |
CONCYTEC |
| institution |
CONCYTEC |
| reponame_str |
CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
| repository.name.fl_str_mv |
Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1844883020747636736 |
| spelling |
Publicationrp02465600rp02502600rp02503600rp02500600rp00892500rp02501600rp02362500rp02504600rp01358500rp00575500Valdivia M.Castañeda-Zegarra S.Lévano G.Lazo J.Reyes J.Bravo Z.Santiani A.Mujica F.Ruíz J.Gonzales G.F.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2019https://hdl.handle.net/20.500.12390/942https://doi.org/10.1111/and.132832-s2.0-85063990103This study was supported by Grant PIBA15A106 and B17100101 Universidad Nacional Mayor de San Marcos and National Council of Science Technology and Innovation N° 313-2011-CONCYTEC-OAJ. This article includes results of Martha Valdivia ?octoral Thesis of Sciences with mention of Physiology, Universidad Peruana CayetanoThe identification system of spermatogonial stem cell (SSC) was established in alpaca using the molecular expression as well as the reactivity pattern to Dolichos biflorus agglutinin (DBA) by flow cytometry. Twenty-four testicles with their epididymis were recovered from adult alpacas at the slaughterhouse of Huancavelica—Perú. Samples were transported to the Laboratory of Reproductive Physiology at Universidad Nacional Mayor de San Marcos. Testes were selected for our study when the progressive motility of epididymal spermatozoa (ESPM) was above 30%. Isolation of SSC was performed with two enzymatic digestions. Finally, sperm viability was evaluated by means of the trypan blue vital stain in spermatogonial round cells. Samples with more than 80% viability were selected. Isolated cells cultured for 2 days were used for identifying the presence of SSCs by the expression of integrin β1 (116 bp) and PLZF (206 bp) genes. Spermatogonia were classified according to the DBA reactivity. Spermatogonia with a strong positive to DBA (sDBA+) were classified as SSC (Mean ± SEM=4.44 ± 0.68%). Spermatogonia in early differentiation stages stained weakly positive with DBA (wDBA+) (Mean ± SEM=37.44 ± 3.07%) and differentiated round cells as DBA negative (Mean ± SEM=54.12 ± 3.18%). With the use of molecular and DBA markers, it is possible to identify easily the spermatogonial stem cells in alpaca.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengBlackwell Publishing Ltdinfo:eu-repo/semantics/openAccessSSCAlpaca-1DBA-1epididymal sperm mobility-1molecular markers-1https://purl.org/pe-repo/ocde/ford#1.06.03-1Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testesinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#20.500.12390/942oai:repositorio.concytec.gob.pe:20.500.12390/9422024-05-30 15:23:16.672http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="b864190f-754a-4fd3-8c3f-e07a0eb65792"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2019</PublicationDate> <DOI>https://doi.org/10.1111/and.13283</DOI> <SCP-Number>2-s2.0-85063990103</SCP-Number> <Authors> <Author> <DisplayName>Valdivia M.</DisplayName> <Person id="rp02465" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Castañeda-Zegarra S.</DisplayName> <Person id="rp02502" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Lévano G.</DisplayName> <Person id="rp02503" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Lazo J.</DisplayName> <Person id="rp02500" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Reyes J.</DisplayName> <Person id="rp00892" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Bravo Z.</DisplayName> <Person id="rp02501" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Santiani A.</DisplayName> <Person id="rp02362" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Mujica F.</DisplayName> <Person id="rp02504" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Ruíz J.</DisplayName> <Person id="rp01358" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Gonzales G.F.</DisplayName> <Person id="rp00575" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Blackwell Publishing Ltd</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>SSC</Keyword> <Keyword>Alpaca</Keyword> <Keyword>DBA</Keyword> <Keyword>epididymal sperm mobility</Keyword> <Keyword>molecular markers</Keyword> <Abstract>The identification system of spermatogonial stem cell (SSC) was established in alpaca using the molecular expression as well as the reactivity pattern to Dolichos biflorus agglutinin (DBA) by flow cytometry. Twenty-four testicles with their epididymis were recovered from adult alpacas at the slaughterhouse of Huancavelica—Perú. Samples were transported to the Laboratory of Reproductive Physiology at Universidad Nacional Mayor de San Marcos. Testes were selected for our study when the progressive motility of epididymal spermatozoa (ESPM) was above 30%. Isolation of SSC was performed with two enzymatic digestions. Finally, sperm viability was evaluated by means of the trypan blue vital stain in spermatogonial round cells. Samples with more than 80% viability were selected. Isolated cells cultured for 2 days were used for identifying the presence of SSCs by the expression of integrin β1 (116 bp) and PLZF (206 bp) genes. Spermatogonia were classified according to the DBA reactivity. Spermatogonia with a strong positive to DBA (sDBA+) were classified as SSC (Mean ± SEM=4.44 ± 0.68%). Spermatogonia in early differentiation stages stained weakly positive with DBA (wDBA+) (Mean ± SEM=37.44 ± 3.07%) and differentiated round cells as DBA negative (Mean ± SEM=54.12 ± 3.18%). With the use of molecular and DBA markers, it is possible to identify easily the spermatogonial stem cells in alpaca.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
| score |
13.275274 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
