Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples

Descripción del Articulo

The objective of this study was to determine the detection limit of three PCR approaches both from blood samples as well as from filter papers. Furthermore, the specificity was also accessed. We found that the best PCR approach studied was the amplification of the 16S rRNA from blood samples with a...

Descripción completa

Detalles Bibliográficos
Autores: Gomes, C, Martinez-Puchol, S, Pons, MJ, Bazan, J, Tinco, C, del Valle, J, Ruiz, J
Formato: artículo
Fecha de Publicación:2016
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/1063
Enlace del recurso:https://hdl.handle.net/20.500.12390/1063
https://doi.org/10.1371/journal.pntd.0004529
Nivel de acceso:acceso abierto
Materia:Enfermedades infecciosas
Enfermedad de la piel
https://purl.org/pe-repo/ocde/ford#3.03.08
id CONC_6131b33d6db9736e88b5563f2179b235
oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/1063
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
title Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
spellingShingle Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
Gomes, C
Enfermedades infecciosas
Enfermedad de la piel
https://purl.org/pe-repo/ocde/ford#3.03.08
title_short Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
title_full Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
title_fullStr Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
title_full_unstemmed Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
title_sort Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
author Gomes, C
author_facet Gomes, C
Martinez-Puchol, S
Pons, MJ
Bazan, J
Tinco, C
del Valle, J
Ruiz, J
author_role author
author2 Martinez-Puchol, S
Pons, MJ
Bazan, J
Tinco, C
del Valle, J
Ruiz, J
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, C
Martinez-Puchol, S
Pons, MJ
Bazan, J
Tinco, C
del Valle, J
Ruiz, J
dc.subject.none.fl_str_mv Enfermedades infecciosas
topic Enfermedades infecciosas
Enfermedad de la piel
https://purl.org/pe-repo/ocde/ford#3.03.08
dc.subject.es_PE.fl_str_mv Enfermedad de la piel
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#3.03.08
description The objective of this study was to determine the detection limit of three PCR approaches both from blood samples as well as from filter papers. Furthermore, the specificity was also accessed. We found that the best PCR approach studied was the amplification of the 16S rRNA from blood samples with a detection limit of 5 CFU/μL, the same when using dry blood in filter paper, although the obtained bands were not so evident. Present results highlight the need to develop more sensitive techniques able to be used both in rural areas and in the detection of asymptomatic carriers. From the tested PCRs, the 16S rRNA PCR-approach is the best to be used in the direct blood detection of acute cases of Carrion’s disease.
publishDate 2016
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/1063
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1371/journal.pntd.0004529
dc.identifier.isi.none.fl_str_mv 373272500052
url https://hdl.handle.net/20.500.12390/1063
https://doi.org/10.1371/journal.pntd.0004529
identifier_str_mv 373272500052
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv PLOS Neglected Tropical Diseases
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.none.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844883013111906304
spelling Publicationrp01359500rp02997600rp01161500rp01055500rp03022600rp03021600rp01358400Gomes, CMartinez-Puchol, SPons, MJBazan, JTinco, Cdel Valle, JRuiz, J2024-05-30T23:13:38Z2024-05-30T23:13:38Z2016https://hdl.handle.net/20.500.12390/1063https://doi.org/10.1371/journal.pntd.0004529373272500052The objective of this study was to determine the detection limit of three PCR approaches both from blood samples as well as from filter papers. Furthermore, the specificity was also accessed. We found that the best PCR approach studied was the amplification of the 16S rRNA from blood samples with a detection limit of 5 CFU/μL, the same when using dry blood in filter paper, although the obtained bands were not so evident. Present results highlight the need to develop more sensitive techniques able to be used both in rural areas and in the detection of asymptomatic carriers. From the tested PCRs, the 16S rRNA PCR-approach is the best to be used in the direct blood detection of acute cases of Carrion’s disease.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengPublic Library of Science (PLOS)PLOS Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/Enfermedades infecciosasEnfermedad de la piel-1https://purl.org/pe-repo/ocde/ford#3.03.08-1Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samplesinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/1063oai:repositorio.concytec.gob.pe:20.500.12390/10632024-05-30 16:00:51.163https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="a9d45968-b751-4245-8e61-31001aa0861e"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples</Title> <PublishedIn> <Publication> <Title>PLOS Neglected Tropical Diseases</Title> </Publication> </PublishedIn> <PublicationDate>2016</PublicationDate> <DOI>https://doi.org/10.1371/journal.pntd.0004529</DOI> <ISI-Number>373272500052</ISI-Number> <Authors> <Author> <DisplayName>Gomes, C</DisplayName> <Person id="rp01359" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Martinez-Puchol, S</DisplayName> <Person id="rp02997" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Pons, MJ</DisplayName> <Person id="rp01161" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Bazan, J</DisplayName> <Person id="rp01055" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Tinco, C</DisplayName> <Person id="rp03022" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>del Valle, J</DisplayName> <Person id="rp03021" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Ruiz, J</DisplayName> <Person id="rp01358" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Public Library of Science (PLOS)</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by-nc-nd/4.0/</License> <Keyword>Enfermedades infecciosas</Keyword> <Keyword>Enfermedad de la piel</Keyword> <Abstract>The objective of this study was to determine the detection limit of three PCR approaches both from blood samples as well as from filter papers. Furthermore, the specificity was also accessed. We found that the best PCR approach studied was the amplification of the 16S rRNA from blood samples with a detection limit of 5 CFU/μL, the same when using dry blood in filter paper, although the obtained bands were not so evident. Present results highlight the need to develop more sensitive techniques able to be used both in rural areas and in the detection of asymptomatic carriers. From the tested PCRs, the 16S rRNA PCR-approach is the best to be used in the direct blood detection of acute cases of Carrion’s disease.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.394035
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

Ejemplares Similares