Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama) [Neutralización de la actividad letal del veneno de serpiente bothrops atrox por suero hiperinmune de llama (Lama glama)]

• Autores: Calderon H.B., Alave E.G.C., Coronel V.O.Y., Rojas C.P., Pérez M.G., Rey O.A.C., Ferreyra C.B., Felix B.T., Neyra D.G., Arellano R.R.I., Ormachea S.S., Arevalo H.M.
• Formato: artículo
• Fecha de Publicación: 2020
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/2527
• Enlace del recurso: https://hdl.handle.net/20.500.12390/2527; https://doi.org/10.17843/rpmesp.2020.373.4597
• Nivel de acceso: acceso abierto
• Materia: Neutralization; Bothrops atrox; Immune Sera; Lama glama; Lethal Dose 50; Mortality (Source: MeSH NLM); http://purl.org/pe-repo/ocde/ford#3.02.27

Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50 ) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 ?g/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 ?L/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice.