Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]

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The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentati...

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Detalles Bibliográficos
Autores: Terrones K.T., Carranza G.R., Fabián M.B.
Formato: artículo
Fecha de Publicación:2019
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/2710
Enlace del recurso:https://hdl.handle.net/20.500.12390/2710
https://doi.org/10.17843/rpmesp.2019.362.4151
Nivel de acceso:acceso abierto
Materia:Tube sedimentation
Density gradient
Giardia spp
Sucrose
http://purl.org/pe-repo/ocde/ford#1.06.10
id CONC_1aa30deb20cbeecff52673a346bdcad6
oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/2710
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
title Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
spellingShingle Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
Terrones K.T.
Tube sedimentation
Density gradient
Density gradient
Giardia spp
Giardia spp
Sucrose
Sucrose
http://purl.org/pe-repo/ocde/ford#1.06.10
title_short Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
title_full Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
title_fullStr Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
title_full_unstemmed Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
title_sort Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
author Terrones K.T.
author_facet Terrones K.T.
Carranza G.R.
Fabián M.B.
author_role author
author2 Carranza G.R.
Fabián M.B.
author2_role author
author
dc.contributor.author.fl_str_mv Terrones K.T.
Carranza G.R.
Fabián M.B.
dc.subject.none.fl_str_mv Tube sedimentation
topic Tube sedimentation
Density gradient
Density gradient
Giardia spp
Giardia spp
Sucrose
Sucrose
http://purl.org/pe-repo/ocde/ford#1.06.10
dc.subject.es_PE.fl_str_mv Density gradient
Density gradient
Giardia spp
Giardia spp
Sucrose
Sucrose
dc.subject.ocde.none.fl_str_mv http://purl.org/pe-repo/ocde/ford#1.06.10
description The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp. © 2019, Instituto Nacional de Salud. All rights reserved.
publishDate 2019
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/2710
dc.identifier.doi.none.fl_str_mv https://doi.org/10.17843/rpmesp.2019.362.4151
dc.identifier.scopus.none.fl_str_mv 2-s2.0-85071186315
url https://hdl.handle.net/20.500.12390/2710
https://doi.org/10.17843/rpmesp.2019.362.4151
identifier_str_mv 2-s2.0-85071186315
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Revista Peruana de Medicina Experimental y Salud Publica
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.none.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.publisher.none.fl_str_mv Instituto Nacional de Salud
publisher.none.fl_str_mv Instituto Nacional de Salud
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
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spelling Publicationrp07214600rp07212600rp07213600Terrones K.T.Carranza G.R.Fabián M.B.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2019https://hdl.handle.net/20.500.12390/2710https://doi.org/10.17843/rpmesp.2019.362.41512-s2.0-85071186315The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp. © 2019, Instituto Nacional de Salud. All rights reserved.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Publicainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/Tube sedimentationDensity gradient-1Density gradient-1Giardia spp-1Giardia spp-1Sucrose-1Sucrose-1http://purl.org/pe-repo/ocde/ford#1.06.10-1Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]info:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/2710oai:repositorio.concytec.gob.pe:20.500.12390/27102024-05-30 16:10:41.914https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="0fe19a5a-561c-4bfb-a956-a3272b7fbd7e"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]</Title> <PublishedIn> <Publication> <Title>Revista Peruana de Medicina Experimental y Salud Publica</Title> </Publication> </PublishedIn> <PublicationDate>2019</PublicationDate> <DOI>https://doi.org/10.17843/rpmesp.2019.362.4151</DOI> <SCP-Number>2-s2.0-85071186315</SCP-Number> <Authors> <Author> <DisplayName>Terrones K.T.</DisplayName> <Person id="rp07214" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Carranza G.R.</DisplayName> <Person id="rp07212" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Fabián M.B.</DisplayName> <Person id="rp07213" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Instituto Nacional de Salud</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by-nc-nd/4.0/</License> <Keyword>Tube sedimentation</Keyword> <Keyword>Density gradient</Keyword> <Keyword>Density gradient</Keyword> <Keyword>Giardia spp</Keyword> <Keyword>Giardia spp</Keyword> <Keyword>Sucrose</Keyword> <Keyword>Sucrose</Keyword> <Abstract>The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp. © 2019, Instituto Nacional de Salud. All rights reserved.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.4481325
Evaluation of methods of concentration and purification of giardia spp. From coprological samples [Evaluación de métodos de concentración y purificación de giardia spp. A partir de muestras coprológicas]
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