Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process

• Autores: Ccorahua R., Moreno S., Gumz H., Sahre K., Voit B., Appelhans D.
• Formato: artículo
• Fecha de Publicación: 2018
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/756
• Enlace del recurso: https://hdl.handle.net/20.500.12390/756; https://doi.org/10.1039/c8ra03964j
• Nivel de acceso: acceso abierto
• Materia: Therapeutic agents; Biological experiments; Cryogenic freezing; Dispersing process; Freeze-drying process; Functionalized; PH-responsive; Polymersomes; Functional polymers

Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen ( 20 C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor.