Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru

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In the present investigation was able to authenticate the ability to nodulate and to evaluate the frequency of nodulation efficiency of 50 isolate strains of pea nodules, collected from the departments: La Libertad, Lambayeque, Cajamarca and Lima. Which was inoculated to 1 mL of culture suspension a...

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Autores: Moreno-Chirinos, Zoila E., Valdez-Núñez, Renzo A., Soriano-Bernilla, Bertha S., Ruesta-Campoverde, Nelson A.
Formato: artículo
Fecha de Publicación:2016
Institución:Universidad Nacional de Trujillo
Repositorio:Revista UNITRU - Scientia Agropecuaria
Lenguaje:español
OAI Identifier:oai:ojs.revistas.unitru.edu.pe:article/1163
Enlace del recurso:http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1163
Nivel de acceso:acceso abierto
Materia:Rhizobium
Pisum sativum
autenticación
eficiencia en la nodulación
authentication
nodulation efficiency
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spelling Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of PeruEficiencia en la nodulación por rizobios nativos, procedentes de nódulos de Pisum sativum "arveja" colectados de diferentes Departamentos del PerúMoreno-Chirinos, Zoila E.Valdez-Núñez, Renzo A.Soriano-Bernilla, Bertha S.Ruesta-Campoverde, Nelson A.RhizobiumPisum sativumautenticacióneficiencia en la nodulaciónRhizobiumPisum sativumauthenticationnodulation efficiencyIn the present investigation was able to authenticate the ability to nodulate and to evaluate the frequency of nodulation efficiency of 50 isolate strains of pea nodules, collected from the departments: La Libertad, Lambayeque, Cajamarca and Lima. Which was inoculated to 1 mL of culture suspension alone (1.2x109 cel /mL) in seedlings of Pisum sativum var. Selection Junín, keeping them under conditions of relative humidity,50-70% and temperature / photoperiod, 28 °C – 24 °C / 14 h – 10 h (light-dark). At 25 days post-inoculation was obtained that 40% nodulated the host plant. To determine the frequency of nodulation efficiency, we took into account the parameters of speed of nodulation (DN in mm) and the percentage of roots nodulated (PRN%); pearson correlational analysis of the 5% significance, MANOVA and Tukey statistical DHS, allowed to establish the ranges (high, medium and low) for DN and PRN% for each crop, resulting in only 10% of all crops (Ra Ra 218-01 and 212-01) showed high efficiency in nodulation, as DN were high and high PRN%.En la presente investigación se logró autenticar la capacidad nodulativa y evaluar la frecuencia de la eficiencia en la nodulación de 50 cultivos aislados de nódulos radiculares de arveja, colectados de los Departamentos: La Libertad, Lambayeque, Cajamarca y Lima. Para lo cual se inoculó 1mL de suspensión del cultivo aislado (1,2x109 cel / mL) en plántulas de Pisum sativum var. Selección Junín, manteniéndolas bajo condiciones de humedad relativa, 50–70% y temperatura/fotoperiodo, 28 °C - 24 °C / 14 h – 10 h (luz-oscuridad). A los 25 días post-inoculación se obtuvo que el 40% nodularon a la planta huésped. Para determinar la frecuencia en la eficiencia de nodulación, se tuvo en cuenta los parámetros de velocidad de nodulación (DN en mm) y el porcentaje de raíces noduladas (PRN%); el análisis Correlacional de Pearson al 5% de significancia, el MANOVA y el estadístico DHS de Tukey, permitieron establecer los rangos (alta, intermedia y baja) para DN y PRN% por cada cultivo, resultando que solo el 10% de los cultivos evaluados (Ra 218-01 y Ra 212-01) presentaron alta eficiencia en la nodulación, ya que obtuvieron alta DN y alto PRN%.Universidad Nacional de Trujillo2016-09-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/116310.17268/sci.agropecu.2016.03.02Scientia Agropecuaria; Vol. 7 (2016): Special Issue; 165-172Scientia Agropecuaria; Vol. 7 (2016): Número especial; 165-1722306-67412077-9917reponame:Revista UNITRU - Scientia Agropecuariainstname:Universidad Nacional de Trujilloinstacron:UNITRUspahttp://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1163/1101http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1163/1215Derechos de autor 2016 Scientia Agropecuariainfo:eu-repo/semantics/openAccess2021-06-01T15:35:20Zmail@mail.com -
dc.title.none.fl_str_mv Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
Eficiencia en la nodulación por rizobios nativos, procedentes de nódulos de Pisum sativum "arveja" colectados de diferentes Departamentos del Perú
title Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
spellingShingle Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
Moreno-Chirinos, Zoila E.
Rhizobium
Pisum sativum
autenticación
eficiencia en la nodulación
Rhizobium
Pisum sativum
authentication
nodulation efficiency
title_short Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
title_full Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
title_fullStr Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
title_full_unstemmed Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
title_sort Nodulation efficiency by native rhizobia from nodules of Pisum sativum "pea" collected from different Departments of Peru
dc.creator.none.fl_str_mv Moreno-Chirinos, Zoila E.
Valdez-Núñez, Renzo A.
Soriano-Bernilla, Bertha S.
Ruesta-Campoverde, Nelson A.
author Moreno-Chirinos, Zoila E.
author_facet Moreno-Chirinos, Zoila E.
Valdez-Núñez, Renzo A.
Soriano-Bernilla, Bertha S.
Ruesta-Campoverde, Nelson A.
author_role author
author2 Valdez-Núñez, Renzo A.
Soriano-Bernilla, Bertha S.
Ruesta-Campoverde, Nelson A.
author2_role author
author
author
dc.subject.none.fl_str_mv Rhizobium
Pisum sativum
autenticación
eficiencia en la nodulación
Rhizobium
Pisum sativum
authentication
nodulation efficiency
topic Rhizobium
Pisum sativum
autenticación
eficiencia en la nodulación
Rhizobium
Pisum sativum
authentication
nodulation efficiency
dc.description.none.fl_txt_mv In the present investigation was able to authenticate the ability to nodulate and to evaluate the frequency of nodulation efficiency of 50 isolate strains of pea nodules, collected from the departments: La Libertad, Lambayeque, Cajamarca and Lima. Which was inoculated to 1 mL of culture suspension alone (1.2x109 cel /mL) in seedlings of Pisum sativum var. Selection Junín, keeping them under conditions of relative humidity,50-70% and temperature / photoperiod, 28 °C – 24 °C / 14 h – 10 h (light-dark). At 25 days post-inoculation was obtained that 40% nodulated the host plant. To determine the frequency of nodulation efficiency, we took into account the parameters of speed of nodulation (DN in mm) and the percentage of roots nodulated (PRN%); pearson correlational analysis of the 5% significance, MANOVA and Tukey statistical DHS, allowed to establish the ranges (high, medium and low) for DN and PRN% for each crop, resulting in only 10% of all crops (Ra Ra 218-01 and 212-01) showed high efficiency in nodulation, as DN were high and high PRN%.
En la presente investigación se logró autenticar la capacidad nodulativa y evaluar la frecuencia de la eficiencia en la nodulación de 50 cultivos aislados de nódulos radiculares de arveja, colectados de los Departamentos: La Libertad, Lambayeque, Cajamarca y Lima. Para lo cual se inoculó 1mL de suspensión del cultivo aislado (1,2x109 cel / mL) en plántulas de Pisum sativum var. Selección Junín, manteniéndolas bajo condiciones de humedad relativa, 50–70% y temperatura/fotoperiodo, 28 °C - 24 °C / 14 h – 10 h (luz-oscuridad). A los 25 días post-inoculación se obtuvo que el 40% nodularon a la planta huésped. Para determinar la frecuencia en la eficiencia de nodulación, se tuvo en cuenta los parámetros de velocidad de nodulación (DN en mm) y el porcentaje de raíces noduladas (PRN%); el análisis Correlacional de Pearson al 5% de significancia, el MANOVA y el estadístico DHS de Tukey, permitieron establecer los rangos (alta, intermedia y baja) para DN y PRN% por cada cultivo, resultando que solo el 10% de los cultivos evaluados (Ra 218-01 y Ra 212-01) presentaron alta eficiencia en la nodulación, ya que obtuvieron alta DN y alto PRN%.
description In the present investigation was able to authenticate the ability to nodulate and to evaluate the frequency of nodulation efficiency of 50 isolate strains of pea nodules, collected from the departments: La Libertad, Lambayeque, Cajamarca and Lima. Which was inoculated to 1 mL of culture suspension alone (1.2x109 cel /mL) in seedlings of Pisum sativum var. Selection Junín, keeping them under conditions of relative humidity,50-70% and temperature / photoperiod, 28 °C – 24 °C / 14 h – 10 h (light-dark). At 25 days post-inoculation was obtained that 40% nodulated the host plant. To determine the frequency of nodulation efficiency, we took into account the parameters of speed of nodulation (DN in mm) and the percentage of roots nodulated (PRN%); pearson correlational analysis of the 5% significance, MANOVA and Tukey statistical DHS, allowed to establish the ranges (high, medium and low) for DN and PRN% for each crop, resulting in only 10% of all crops (Ra Ra 218-01 and 212-01) showed high efficiency in nodulation, as DN were high and high PRN%.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1163
10.17268/sci.agropecu.2016.03.02
url http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1163
identifier_str_mv 10.17268/sci.agropecu.2016.03.02
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1163/1101
http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1163/1215
dc.rights.none.fl_str_mv Derechos de autor 2016 Scientia Agropecuaria
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2016 Scientia Agropecuaria
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de Trujillo
publisher.none.fl_str_mv Universidad Nacional de Trujillo
dc.source.none.fl_str_mv Scientia Agropecuaria; Vol. 7 (2016): Special Issue; 165-172
Scientia Agropecuaria; Vol. 7 (2016): Número especial; 165-172
2306-6741
2077-9917
reponame:Revista UNITRU - Scientia Agropecuaria
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instacron:UNITRU
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collection Revista UNITRU - Scientia Agropecuaria
instname_str Universidad Nacional de Trujillo
instacron_str UNITRU
institution UNITRU
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