Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
Descripción del Articulo
In the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the...
| Autores: | , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revista UNMSM - Revista Peruana de Biología |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/17875 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875 |
| Nivel de acceso: | acceso abierto |
| Materia: | COI Molecular phylogeny authentication Ampullariidae Amazonia DNA barcoding molecular identification Taxonomy Species identification apple snails Filogenia molecular autenticación código de barras de ADN identificación molecular taxonomía identificación de especie churo negro |
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Revista UNMSM - Revista Peruana de Biología |
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Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia Identificación molecular y relaciones evolutivas de Pomacea nobilis, base para la autenticación específica del churo negro de la Amazonia peruana |
| title |
Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia |
| spellingShingle |
Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia Ramírez, Rina COI Molecular phylogeny authentication Ampullariidae Amazonia DNA barcoding molecular identification Taxonomy Species identification apple snails Filogenia molecular COI autenticación Ampullariidae Amazonia código de barras de ADN identificación molecular taxonomía identificación de especie churo negro |
| title_short |
Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia |
| title_full |
Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia |
| title_fullStr |
Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia |
| title_full_unstemmed |
Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia |
| title_sort |
Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia |
| dc.creator.none.fl_str_mv |
Ramírez, Rina Solis, Maria Ampuero, André Morín, Jaime Jimenez-Vasquez, Victor Ramirez, Jorge L. Congrains, Carlos Temoche, Haydee Shiga, Betty |
| author |
Ramírez, Rina |
| author_facet |
Ramírez, Rina Solis, Maria Ampuero, André Morín, Jaime Jimenez-Vasquez, Victor Ramirez, Jorge L. Congrains, Carlos Temoche, Haydee Shiga, Betty |
| author_role |
author |
| author2 |
Solis, Maria Ampuero, André Morín, Jaime Jimenez-Vasquez, Victor Ramirez, Jorge L. Congrains, Carlos Temoche, Haydee Shiga, Betty |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
COI Molecular phylogeny authentication Ampullariidae Amazonia DNA barcoding molecular identification Taxonomy Species identification apple snails Filogenia molecular COI autenticación Ampullariidae Amazonia código de barras de ADN identificación molecular taxonomía identificación de especie churo negro |
| topic |
COI Molecular phylogeny authentication Ampullariidae Amazonia DNA barcoding molecular identification Taxonomy Species identification apple snails Filogenia molecular COI autenticación Ampullariidae Amazonia código de barras de ADN identificación molecular taxonomía identificación de especie churo negro |
| dc.description.none.fl_txt_mv |
In the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the species Pomacea maculata is mentioned in the literature. Molecular identification was carried out based on the mitochondrial marker COI of individuals of "churo negro" apple snails (Pomacea) commercialized in the markets of Iquitos, as well as those used in restaurant dishes in the city of Lima, and contrasted with specimens from their natural habitat. It was found that these specimens, correspond to the species Pomacea nobilis (Reeve, 1856). The molecular phylogenetic analysis showed P. nobilis as the sister species of P. guyanensis, in the P. glauca group, distantly related to P. maculata. The uncorrected distances found between them, for the mitochondrial marker COI, were from 11.33% to 13.17%, while with P. maculate were from 13.67% to 15.33%. These results demonstrated the effectiveness of the DNA barcode for the identification and authentication of the species, which gives it added value for its eventual export trade. En la Amazonia Peruana los caracoles dulceacuícolas de la familia Ampullariidae son conocidos como churos y originalmente han sido descritas para Perú alrededor de 20 especies. Aunque son muy usadas para alimentación, medicina tradicional y objeto de muchos estudios para su cultivo e industrialización, solamente es mencionada en la literatura la especie Pomacea maculata. Se llevó a cabo la identificación molecular sobre la base del marcador mitocondrial COI, de individuos de churos negros (Pomacea) comercializados en los mercados de Iquitos, así como los usados en platos a la carta en la ciudad de Lima, contrastados con otros individuos de procedencia de su hábitat natural. Se encontró que estos especímenes expendidos corresponden a la especie Pomacea nobilis (Reeve, 1856). El análisis filogenético molecular mostró que P. nobilis es especie hermana de P. guyanensis, en el grupo de P. glauca, distantemente relacionada de P. maculata. Las distancias no corregidas encontradas entre ellas, para el marcador mitocondrial COI, fueron de 11.33% a 13.17%, mientras que con P. maculata fueron de 13.67% a 15.33%. Estos resultados demostraron la eficacia del código de barras de ADN para la identificación y autenticación de la especie, lo que le da un valor agregado para su eventual comercio de exportación. |
| description |
In the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the species Pomacea maculata is mentioned in the literature. Molecular identification was carried out based on the mitochondrial marker COI of individuals of "churo negro" apple snails (Pomacea) commercialized in the markets of Iquitos, as well as those used in restaurant dishes in the city of Lima, and contrasted with specimens from their natural habitat. It was found that these specimens, correspond to the species Pomacea nobilis (Reeve, 1856). The molecular phylogenetic analysis showed P. nobilis as the sister species of P. guyanensis, in the P. glauca group, distantly related to P. maculata. The uncorrected distances found between them, for the mitochondrial marker COI, were from 11.33% to 13.17%, while with P. maculate were from 13.67% to 15.33%. These results demonstrated the effectiveness of the DNA barcode for the identification and authentication of the species, which gives it added value for its eventual export trade. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-05-24 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875 10.15381/rpb.v27i2.17875 |
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https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875 |
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10.15381/rpb.v27i2.17875 |
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spa |
| language |
spa |
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https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875/14932 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2020 Rina Ramírez, Maria Solis, André Ampuero, Jaime Morín, Victor Jimenez-Vasquez, Jorge L. Ramirez, Carlos Congrains, Haydee Temoche, Betty Shiga http://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2020 Rina Ramírez, Maria Solis, André Ampuero, Jaime Morín, Victor Jimenez-Vasquez, Jorge L. Ramirez, Carlos Congrains, Haydee Temoche, Betty Shiga http://creativecommons.org/licenses/by-nc-sa/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas |
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Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas |
| dc.source.none.fl_str_mv |
Revista Peruana de Biología; Vol 27 No 2 (2020); 139-148 Revista Peruana de Biología; Vol. 27 Núm. 2 (2020); 139-148 1727-9933 1561-0837 10.15381/rpb.v27i2 reponame:Revista UNMSM - Revista Peruana de Biología instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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mail@mail.com |
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Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian AmazoniaIdentificación molecular y relaciones evolutivas de Pomacea nobilis, base para la autenticación específica del churo negro de la Amazonia peruanaRamírez, RinaSolis, MariaAmpuero, AndréMorín, JaimeJimenez-Vasquez, VictorRamirez, Jorge L.Congrains, CarlosTemoche, HaydeeShiga, BettyCOIMolecular phylogenyauthenticationAmpullariidaeAmazoniaDNA barcodingmolecular identificationTaxonomySpecies identificationapple snailsFilogenia molecularCOIautenticaciónAmpullariidaeAmazoniacódigo de barras de ADNidentificación moleculartaxonomíaidentificación de especiechuro negroIn the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the species Pomacea maculata is mentioned in the literature. Molecular identification was carried out based on the mitochondrial marker COI of individuals of "churo negro" apple snails (Pomacea) commercialized in the markets of Iquitos, as well as those used in restaurant dishes in the city of Lima, and contrasted with specimens from their natural habitat. It was found that these specimens, correspond to the species Pomacea nobilis (Reeve, 1856). The molecular phylogenetic analysis showed P. nobilis as the sister species of P. guyanensis, in the P. glauca group, distantly related to P. maculata. The uncorrected distances found between them, for the mitochondrial marker COI, were from 11.33% to 13.17%, while with P. maculate were from 13.67% to 15.33%. These results demonstrated the effectiveness of the DNA barcode for the identification and authentication of the species, which gives it added value for its eventual export trade.En la Amazonia Peruana los caracoles dulceacuícolas de la familia Ampullariidae son conocidos como churos y originalmente han sido descritas para Perú alrededor de 20 especies. Aunque son muy usadas para alimentación, medicina tradicional y objeto de muchos estudios para su cultivo e industrialización, solamente es mencionada en la literatura la especie Pomacea maculata. Se llevó a cabo la identificación molecular sobre la base del marcador mitocondrial COI, de individuos de churos negros (Pomacea) comercializados en los mercados de Iquitos, así como los usados en platos a la carta en la ciudad de Lima, contrastados con otros individuos de procedencia de su hábitat natural. Se encontró que estos especímenes expendidos corresponden a la especie Pomacea nobilis (Reeve, 1856). El análisis filogenético molecular mostró que P. nobilis es especie hermana de P. guyanensis, en el grupo de P. glauca, distantemente relacionada de P. maculata. Las distancias no corregidas encontradas entre ellas, para el marcador mitocondrial COI, fueron de 11.33% a 13.17%, mientras que con P. maculata fueron de 13.67% a 15.33%. Estos resultados demostraron la eficacia del código de barras de ADN para la identificación y autenticación de la especie, lo que le da un valor agregado para su eventual comercio de exportación.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2020-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1787510.15381/rpb.v27i2.17875Revista Peruana de Biología; Vol 27 No 2 (2020); 139-148Revista Peruana de Biología; Vol. 27 Núm. 2 (2020); 139-1481727-99331561-083710.15381/rpb.v27i2reponame:Revista UNMSM - Revista Peruana de Biologíainstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875/14932Derechos de autor 2020 Rina Ramírez, Maria Solis, André Ampuero, Jaime Morín, Victor Jimenez-Vasquez, Jorge L. Ramirez, Carlos Congrains, Haydee Temoche, Betty Shigahttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T17:50:06Zmail@mail.com - |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).