Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia

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In the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the...

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Detalles Bibliográficos
Autores: Ramírez, Rina, Solis, Maria, Ampuero, André, Morín, Jaime, Jimenez-Vasquez, Victor, Ramirez, Jorge L., Congrains, Carlos, Temoche, Haydee, Shiga, Betty
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - Revista Peruana de Biología
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/17875
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875
Nivel de acceso:acceso abierto
Materia:COI
Molecular phylogeny
authentication
Ampullariidae
Amazonia
DNA barcoding
molecular identification
Taxonomy
Species identification
apple snails
Filogenia molecular
autenticación
código de barras de ADN
identificación molecular
taxonomía
identificación de especie
churo negro
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oai_identifier_str oai:ojs.csi.unmsm:article/17875
network_acronym_str 1727-9933
repository_id_str .
network_name_str Revista UNMSM - Revista Peruana de Biología
dc.title.none.fl_str_mv Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
Identificación molecular y relaciones evolutivas de Pomacea nobilis, base para la autenticación específica del churo negro de la Amazonia peruana
title Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
spellingShingle Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
Ramírez, Rina
COI
Molecular phylogeny
authentication
Ampullariidae
Amazonia
DNA barcoding
molecular identification
Taxonomy
Species identification
apple snails
Filogenia molecular
COI
autenticación
Ampullariidae
Amazonia
código de barras de ADN
identificación molecular
taxonomía
identificación de especie
churo negro
title_short Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
title_full Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
title_fullStr Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
title_full_unstemmed Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
title_sort Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian Amazonia
dc.creator.none.fl_str_mv Ramírez, Rina
Solis, Maria
Ampuero, André
Morín, Jaime
Jimenez-Vasquez, Victor
Ramirez, Jorge L.
Congrains, Carlos
Temoche, Haydee
Shiga, Betty
author Ramírez, Rina
author_facet Ramírez, Rina
Solis, Maria
Ampuero, André
Morín, Jaime
Jimenez-Vasquez, Victor
Ramirez, Jorge L.
Congrains, Carlos
Temoche, Haydee
Shiga, Betty
author_role author
author2 Solis, Maria
Ampuero, André
Morín, Jaime
Jimenez-Vasquez, Victor
Ramirez, Jorge L.
Congrains, Carlos
Temoche, Haydee
Shiga, Betty
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COI
Molecular phylogeny
authentication
Ampullariidae
Amazonia
DNA barcoding
molecular identification
Taxonomy
Species identification
apple snails
Filogenia molecular
COI
autenticación
Ampullariidae
Amazonia
código de barras de ADN
identificación molecular
taxonomía
identificación de especie
churo negro
topic COI
Molecular phylogeny
authentication
Ampullariidae
Amazonia
DNA barcoding
molecular identification
Taxonomy
Species identification
apple snails
Filogenia molecular
COI
autenticación
Ampullariidae
Amazonia
código de barras de ADN
identificación molecular
taxonomía
identificación de especie
churo negro
dc.description.none.fl_txt_mv In the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the species Pomacea maculata is mentioned in the literature. Molecular identification was carried out based on the mitochondrial marker COI of individuals of "churo negro" apple snails (Pomacea) commercialized in the markets of Iquitos, as well as those used in restaurant dishes in the city of Lima, and contrasted with specimens from their natural habitat. It was found that these specimens, correspond to the species Pomacea nobilis (Reeve, 1856). The molecular phylogenetic analysis showed P. nobilis as the sister species of P. guyanensis, in the P. glauca group, distantly related to P. maculata. The uncorrected distances found between them, for the mitochondrial marker COI, were from 11.33% to 13.17%, while with P. maculate were from 13.67% to 15.33%. These results demonstrated the effectiveness of the DNA barcode for the identification and authentication of the species, which gives it added value for its eventual export trade.
En la Amazonia Peruana los caracoles dulceacuícolas de la familia Ampullariidae son conocidos como churos y originalmente han sido descritas para Perú alrededor de 20 especies. Aunque son muy usadas para alimentación, medicina tradicional y objeto de muchos estudios para su cultivo e industrialización, solamente es mencionada en la literatura la especie Pomacea maculata. Se llevó a cabo la identificación molecular sobre la base del marcador mitocondrial COI, de individuos de churos negros (Pomacea) comercializados en los mercados de Iquitos, así como los usados en platos a la carta en la ciudad de Lima, contrastados con otros individuos de procedencia de su hábitat natural. Se encontró que estos especímenes expendidos corresponden a la especie Pomacea nobilis (Reeve, 1856). El análisis filogenético molecular mostró que P. nobilis es especie hermana de P. guyanensis, en el grupo de P. glauca, distantemente relacionada de P. maculata. Las distancias no corregidas encontradas entre ellas, para el marcador mitocondrial COI, fueron de 11.33% a 13.17%, mientras que con P. maculata fueron de 13.67% a 15.33%. Estos resultados demostraron la eficacia del código de barras de ADN para la identificación y autenticación de la especie, lo que le da un valor agregado para su eventual comercio de exportación.
description In the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the species Pomacea maculata is mentioned in the literature. Molecular identification was carried out based on the mitochondrial marker COI of individuals of "churo negro" apple snails (Pomacea) commercialized in the markets of Iquitos, as well as those used in restaurant dishes in the city of Lima, and contrasted with specimens from their natural habitat. It was found that these specimens, correspond to the species Pomacea nobilis (Reeve, 1856). The molecular phylogenetic analysis showed P. nobilis as the sister species of P. guyanensis, in the P. glauca group, distantly related to P. maculata. The uncorrected distances found between them, for the mitochondrial marker COI, were from 11.33% to 13.17%, while with P. maculate were from 13.67% to 15.33%. These results demonstrated the effectiveness of the DNA barcode for the identification and authentication of the species, which gives it added value for its eventual export trade.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875
10.15381/rpb.v27i2.17875
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875
identifier_str_mv 10.15381/rpb.v27i2.17875
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875/14932
dc.rights.none.fl_str_mv Derechos de autor 2020 Rina Ramírez, Maria Solis, André Ampuero, Jaime Morín, Victor Jimenez-Vasquez, Jorge L. Ramirez, Carlos Congrains, Haydee Temoche, Betty Shiga
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Rina Ramírez, Maria Solis, André Ampuero, Jaime Morín, Victor Jimenez-Vasquez, Jorge L. Ramirez, Carlos Congrains, Haydee Temoche, Betty Shiga
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol 27 No 2 (2020); 139-148
Revista Peruana de Biología; Vol. 27 Núm. 2 (2020); 139-148
1727-9933
1561-0837
10.15381/rpb.v27i2
reponame:Revista UNMSM - Revista Peruana de Biología
instname:Universidad Nacional Mayor de San Marcos
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reponame_str Revista UNMSM - Revista Peruana de Biología
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instname_str Universidad Nacional Mayor de San Marcos
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institution UNMSM
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repository.mail.fl_str_mv mail@mail.com
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spelling Molecular identification and evolutionary relationships of Pomacea nobilis, basis for the specific authentication of the black apple snail from Peruvian AmazoniaIdentificación molecular y relaciones evolutivas de Pomacea nobilis, base para la autenticación específica del churo negro de la Amazonia peruanaRamírez, RinaSolis, MariaAmpuero, AndréMorín, JaimeJimenez-Vasquez, VictorRamirez, Jorge L.Congrains, CarlosTemoche, HaydeeShiga, BettyCOIMolecular phylogenyauthenticationAmpullariidaeAmazoniaDNA barcodingmolecular identificationTaxonomySpecies identificationapple snailsFilogenia molecularCOIautenticaciónAmpullariidaeAmazoniacódigo de barras de ADNidentificación moleculartaxonomíaidentificación de especiechuro negroIn the Peruvian Amazon, freshwater snails of the Ampullariidae family are known as churos, and around 20 species have originally been described for Peru. Although they are widely used for food, traditional medicine and the object of many studies for their cultivation and industrialization, only the species Pomacea maculata is mentioned in the literature. Molecular identification was carried out based on the mitochondrial marker COI of individuals of "churo negro" apple snails (Pomacea) commercialized in the markets of Iquitos, as well as those used in restaurant dishes in the city of Lima, and contrasted with specimens from their natural habitat. It was found that these specimens, correspond to the species Pomacea nobilis (Reeve, 1856). The molecular phylogenetic analysis showed P. nobilis as the sister species of P. guyanensis, in the P. glauca group, distantly related to P. maculata. The uncorrected distances found between them, for the mitochondrial marker COI, were from 11.33% to 13.17%, while with P. maculate were from 13.67% to 15.33%. These results demonstrated the effectiveness of the DNA barcode for the identification and authentication of the species, which gives it added value for its eventual export trade.En la Amazonia Peruana los caracoles dulceacuícolas de la familia Ampullariidae son conocidos como churos y originalmente han sido descritas para Perú alrededor de 20 especies. Aunque son muy usadas para alimentación, medicina tradicional y objeto de muchos estudios para su cultivo e industrialización, solamente es mencionada en la literatura la especie Pomacea maculata. Se llevó a cabo la identificación molecular sobre la base del marcador mitocondrial COI, de individuos de churos negros (Pomacea) comercializados en los mercados de Iquitos, así como los usados en platos a la carta en la ciudad de Lima, contrastados con otros individuos de procedencia de su hábitat natural. Se encontró que estos especímenes expendidos corresponden a la especie Pomacea nobilis (Reeve, 1856). El análisis filogenético molecular mostró que P. nobilis es especie hermana de P. guyanensis, en el grupo de P. glauca, distantemente relacionada de P. maculata. Las distancias no corregidas encontradas entre ellas, para el marcador mitocondrial COI, fueron de 11.33% a 13.17%, mientras que con P. maculata fueron de 13.67% a 15.33%. Estos resultados demostraron la eficacia del código de barras de ADN para la identificación y autenticación de la especie, lo que le da un valor agregado para su eventual comercio de exportación.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2020-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1787510.15381/rpb.v27i2.17875Revista Peruana de Biología; Vol 27 No 2 (2020); 139-148Revista Peruana de Biología; Vol. 27 Núm. 2 (2020); 139-1481727-99331561-083710.15381/rpb.v27i2reponame:Revista UNMSM - Revista Peruana de Biologíainstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/17875/14932Derechos de autor 2020 Rina Ramírez, Maria Solis, André Ampuero, Jaime Morín, Victor Jimenez-Vasquez, Jorge L. Ramirez, Carlos Congrains, Haydee Temoche, Betty Shigahttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T17:50:06Zmail@mail.com -
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