Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria

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The aim of this study was to evaluate the antibacterial activity of methanol and methanol-chloroform extracts of tegument, gonad and viscera of Pattalus mollis. Fifty specimens were collected in the Pucusana Bay, Peru, dissected longitudinally through the ventral area of ​​the tegument and the inter...

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Autores: Díaz-Sol Sol, Katia, Sánchez-Robinet, Claudia, Pariona V., Carlos, Londoñe-Bailon, Pablo
Formato: artículo
Fecha de Publicación:2019
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/16612
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16612
Nivel de acceso:acceso abierto
Materia:sea cucumber; Pattalus mollis; methanolic extract; antibacterial activity
pepino de mar; Pattalus mollis; extracto metanólico; actividad antibacteriana
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spelling Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteriaActividad antibacteriana de extractos metanólicos de pepino de mar (Pattalus mollis) frente a bacterias patógenasDíaz-Sol Sol, KatiaSánchez-Robinet, ClaudiaPariona V., CarlosLondoñe-Bailon, Pablosea cucumber; Pattalus mollis; methanolic extract; antibacterial activitypepino de mar; Pattalus mollis; extracto metanólico; actividad antibacterianaThe aim of this study was to evaluate the antibacterial activity of methanol and methanol-chloroform extracts of tegument, gonad and viscera of Pattalus mollis. Fifty specimens were collected in the Pucusana Bay, Peru, dissected longitudinally through the ventral area of ​​the tegument and the internal organs (viscera, gonad) were removed. The samples were dried to 10% moisture, milled and stored in freezing. The extracts were obtained by maceration using methanol and chloroform. The in vitro evaluation was performed using the broth microdilution method, the extracts were diluted in seven concentrations (3.9-250 mg/ml) and evaluated against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 14775 and Vibrio alginolyticus ATCC 17749. The methanol extract showed bacterial inhibition against the three bacteria, demonstrating a bactericidal and bacteriostatic effect against Gram-positive and Gram-negative bacteria. The viscera, gonad and tegument extracts showed a minimum inhibitory concentration (MIC) of 62.5 mg/ml or higher against P. aeruginosa, V. alginolyticus and S. aureus. The results suggest that sea cucumber P. mollis, from the Peruvian coasts, is a novel candidate to produce antibacterial compounds.El objetivo de esta investigación fue evaluar la actividad antibacteriana de extractos de metanol y metanol-cloroformo de tegumento, gónada y víscera de Pattalus mollis. Se recolectaron 50 especímenes en la bahía de Pucusana, Perú, se diseccionaron longitudinalmente por la zona ventral del tegumento y se retiraron los órganos internos (víscera, gónada). Las muestras fueron secadas hasta un 10% de humedad, molidas y almacenadas en congelación. Los extractos fueron obtenidos mediante maceración empleando metanol y cloroformo. La evaluación in vitro se realizó mediante el método de microdilución en caldo, los extractos se diluyeron en siete concentraciones (3.9-250 mg/ml) y evaluados frente a Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 14775 y Vibrio alginolyticus ATCC 17749. Los extractos metanólicos presentaron inhibición bacteriana frente a las tres bacterias demostrando poseer un efecto bactericida y bacteriostático contra bacterias grampositivas y gramnegativas. Los extractos de víscera, gónada y tegumento presentaron una concentración mínima inhibitoria (CMI) de 62.5 mg/ml o más frente a P. aeruginosa, V. alginolyticus y S. aureus. Los resultados sugieren que el pepino de mar P. mollis, proveniente de las costas peruana, es un candidato novedoso para la obtención de compuestos antibacterianos.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2019-10-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1661210.15381/rivep.v30i3.16612Revista de Investigaciones Veterinarias del Perú; Vol 30 No 3 (2019); 1257-1266Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 3 (2019); 1257-12661682-34191609-9117reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perúinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16612/14379Derechos de autor 2019 Katia Díaz-Sol Sol, Claudia Sánchez-Robinet, Carlos Pariona V., Pablo Londoñe-Bailonhttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T18:10:12Zmail@mail.com -
dc.title.none.fl_str_mv Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
Actividad antibacteriana de extractos metanólicos de pepino de mar (Pattalus mollis) frente a bacterias patógenas
title Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
spellingShingle Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
Díaz-Sol Sol, Katia
sea cucumber; Pattalus mollis; methanolic extract; antibacterial activity
pepino de mar; Pattalus mollis; extracto metanólico; actividad antibacteriana
title_short Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
title_full Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
title_fullStr Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
title_full_unstemmed Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
title_sort Antibacterial activity of methanolic extracts from sea cucumber (Pattalus mollis) against pathogenic bacteria
dc.creator.none.fl_str_mv Díaz-Sol Sol, Katia
Sánchez-Robinet, Claudia
Pariona V., Carlos
Londoñe-Bailon, Pablo
author Díaz-Sol Sol, Katia
author_facet Díaz-Sol Sol, Katia
Sánchez-Robinet, Claudia
Pariona V., Carlos
Londoñe-Bailon, Pablo
author_role author
author2 Sánchez-Robinet, Claudia
Pariona V., Carlos
Londoñe-Bailon, Pablo
author2_role author
author
author
dc.subject.none.fl_str_mv sea cucumber; Pattalus mollis; methanolic extract; antibacterial activity
pepino de mar; Pattalus mollis; extracto metanólico; actividad antibacteriana
topic sea cucumber; Pattalus mollis; methanolic extract; antibacterial activity
pepino de mar; Pattalus mollis; extracto metanólico; actividad antibacteriana
dc.description.none.fl_txt_mv The aim of this study was to evaluate the antibacterial activity of methanol and methanol-chloroform extracts of tegument, gonad and viscera of Pattalus mollis. Fifty specimens were collected in the Pucusana Bay, Peru, dissected longitudinally through the ventral area of ​​the tegument and the internal organs (viscera, gonad) were removed. The samples were dried to 10% moisture, milled and stored in freezing. The extracts were obtained by maceration using methanol and chloroform. The in vitro evaluation was performed using the broth microdilution method, the extracts were diluted in seven concentrations (3.9-250 mg/ml) and evaluated against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 14775 and Vibrio alginolyticus ATCC 17749. The methanol extract showed bacterial inhibition against the three bacteria, demonstrating a bactericidal and bacteriostatic effect against Gram-positive and Gram-negative bacteria. The viscera, gonad and tegument extracts showed a minimum inhibitory concentration (MIC) of 62.5 mg/ml or higher against P. aeruginosa, V. alginolyticus and S. aureus. The results suggest that sea cucumber P. mollis, from the Peruvian coasts, is a novel candidate to produce antibacterial compounds.
El objetivo de esta investigación fue evaluar la actividad antibacteriana de extractos de metanol y metanol-cloroformo de tegumento, gónada y víscera de Pattalus mollis. Se recolectaron 50 especímenes en la bahía de Pucusana, Perú, se diseccionaron longitudinalmente por la zona ventral del tegumento y se retiraron los órganos internos (víscera, gónada). Las muestras fueron secadas hasta un 10% de humedad, molidas y almacenadas en congelación. Los extractos fueron obtenidos mediante maceración empleando metanol y cloroformo. La evaluación in vitro se realizó mediante el método de microdilución en caldo, los extractos se diluyeron en siete concentraciones (3.9-250 mg/ml) y evaluados frente a Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 14775 y Vibrio alginolyticus ATCC 17749. Los extractos metanólicos presentaron inhibición bacteriana frente a las tres bacterias demostrando poseer un efecto bactericida y bacteriostático contra bacterias grampositivas y gramnegativas. Los extractos de víscera, gónada y tegumento presentaron una concentración mínima inhibitoria (CMI) de 62.5 mg/ml o más frente a P. aeruginosa, V. alginolyticus y S. aureus. Los resultados sugieren que el pepino de mar P. mollis, proveniente de las costas peruana, es un candidato novedoso para la obtención de compuestos antibacterianos.
description The aim of this study was to evaluate the antibacterial activity of methanol and methanol-chloroform extracts of tegument, gonad and viscera of Pattalus mollis. Fifty specimens were collected in the Pucusana Bay, Peru, dissected longitudinally through the ventral area of ​​the tegument and the internal organs (viscera, gonad) were removed. The samples were dried to 10% moisture, milled and stored in freezing. The extracts were obtained by maceration using methanol and chloroform. The in vitro evaluation was performed using the broth microdilution method, the extracts were diluted in seven concentrations (3.9-250 mg/ml) and evaluated against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 14775 and Vibrio alginolyticus ATCC 17749. The methanol extract showed bacterial inhibition against the three bacteria, demonstrating a bactericidal and bacteriostatic effect against Gram-positive and Gram-negative bacteria. The viscera, gonad and tegument extracts showed a minimum inhibitory concentration (MIC) of 62.5 mg/ml or higher against P. aeruginosa, V. alginolyticus and S. aureus. The results suggest that sea cucumber P. mollis, from the Peruvian coasts, is a novel candidate to produce antibacterial compounds.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16612
10.15381/rivep.v30i3.16612
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16612
identifier_str_mv 10.15381/rivep.v30i3.16612
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16612/14379
dc.rights.none.fl_str_mv Derechos de autor 2019 Katia Díaz-Sol Sol, Claudia Sánchez-Robinet, Carlos Pariona V., Pablo Londoñe-Bailon
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2019 Katia Díaz-Sol Sol, Claudia Sánchez-Robinet, Carlos Pariona V., Pablo Londoñe-Bailon
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol 30 No 3 (2019); 1257-1266
Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 3 (2019); 1257-1266
1682-3419
1609-9117
reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
reponame_str Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
collection Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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score 13.905835
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