Extraction by biocatalytic action and quantification of lutein from tomato of tree (Cyphomandra betacea)

• Autores: Córdova, Javier S., Roque, Blanca L.
• Formato: artículo
• Fecha de Publicación: 2014
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revista UNMSM - Ciencia e Investigación
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/11089
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/11089
• Nivel de acceso: acceso abierto
• Materia: Biocatalytic extraction; lutein; carotenoids; tree tomato; Extracción biocatalítica; luteína; carotenoides; tomate de árbol

The amount of oleoresin was determined and the bioactive components were quantified by high efficiency liquid chromatograph (HPLC) obtaining, in the chromatograms, lutein, beta-carotene and licopene in large quantities. After determination of fruit maturity index, the fruits were conditioning –it was 4,71–; then were dehydrated at 40°C for 96 hours on a tray dryer, and were ground and sieved by) a mesh with diameter smaller than 425 μm. Subsequently, the cell walls were hydrolyzed to extract lutein from dry tissue using a cellulose enzyme complex. Following leaching was performed using a mixture of hexane, ethanol, acetone and toluene in a ratio of 10:6:7: 7 (v/v/v/v) at a temperature of 35°C for a time of 3 hours. The third treatment gave better results in the extraction of lutein, this was performed at 30°C for 4 hours and substrate:enzyme ratio 1:16. A yield of 98,30 mg lutein / 100g of dry matter was obtained. The identified chromatographic peaks of lutein, β-carotene and lycopene, had characteristic spectra with wavelengths of maximum absorption of 446, 452 and 471 ŋm, respectively.