The mite Varroa destructor Delfinado & Baker, 1974 is the most serious pest of honey bee colonies worldwide. This species has become a major plague for world beekeeping of European bees (Apis mellifera Linnaeus, 1758), although the impact from mite infestations varies greatly between continents. The objective of this study was to calculate the risk for varroosis after the application, individually and jointly, of three methods: honeycomb trap, queen replacement and best practices; as well as to assess the economic losses in the province of Villa Clara, Cuba. A total of 467 apiaries were investigated, three hives were sampled per apiary, for a sample size of 3,257 individuals. A retrospective descriptive epidemiological analysis of the behavior of the occurrence of varroosis was carried out. A chronological series of three years was analyzed and the seasonality, channels of habitual b...