El sector de la construcción constituye uno de los pilares fundamentales del crecimiento económico nacional, contribuyendo con más del 8% al Producto Bruto Interno (PBI) y fomentando indirectamente la actividad de más de ocho subsectores gracias a su amplio efecto multiplicador laboral (CEMEX, 2020). En este contexto, resulta esencial comprender el comportamiento del concreto, material preponderantemente utilizado, que debe adaptarse a las exigencias globales actuales de sostenibilidad ambiental y viabilidad económica. Perú, uno de los países severamente impactados por la pandemia del COVID-19, enfrenta retos persistentes no solo en el plano económico sino también ambiental tras la conclusión de esta crisis sanitaria (BBC News Mundo, 2020). Un desafío particularmente destacable es la gestión adecuada de las mascarillas desechables, cuyo uso masivo, impulsado por normativas de...

Tuberculosis and brucellosis remain important causes of morbidity and mortality in many countries, for the detection of both diseases requires efficient and sensitive tool for effectuate the diagnosis. This study was aimed to evaluate and compare the duplex PCR versus the nested PCR, for detection of Brucella spp. (BR) and Mycobacterium spp. (TB). A total of 100 samples of tissues from tracheo-bronchial lymph nodes, bovine lung and bacterial isolate as positive controls were used. Were evaluated ten combinations of primers which were designed to flank the segment of the 16S rRNA sequence (RB) and antigen gen MPB70 (TB), the best result for the Duplex PCR was obtained with the primers Bru-2F/Bru-2R for BR and Tub-1F/Tub-N-R for TB. The amplification of the products was 225 and 230-bp respectively. In order to compare the results of the proposed technique, all samples were initially analyz...

Tuberculosis and brucellosis remain important causes of morbidity and mortality in many countries, for the detection of both diseases requires efficient and sensitive tool for effectuate the diagnosis. This study was aimed to evaluate and compare the duplex PCR versus the nested PCR, for detection of Brucella spp. (BR) and Mycobacterium spp. (TB). A total of 100 samples of tissues from tracheo-bronchial lymph nodes, bovine lung and bacterial isolate as positive controls were used. Were evaluated ten combinations of primers which were designed to flank the segment of the 16S rRNA sequence (RB) and antigen gen MPB70 (TB), the best result for the Duplex PCR was obtained with the primers Bru-2F/Bru-2R for BR and Tub-1F/Tub-N-R for TB. The amplification of the products was 225 and 230-bp respectively. In order to compare the results of the proposed technique, all samples were initially analyz...