This experimental research aimed to have and cultivate stem cells from pulp tissue of impacted third molars, from 20 volunteers of both genders aged between 18 and 22 years who meet the inclusion criteria and gave their consent to voluntary participation.The laboratory method comprises successive steps that begin with obtaining the sample imediata pulp for conservation in the standardized average Dullbecos Modified Eagle Medium® culture supplemented with fetal bovine serum and antibiotics solution. The technique used to obtain stem cells by enzymatic digestion was given by Gronthos et al; the expected density of cell culture was achieved after 9 days to proceed to trypsinization and continue their cultivation; then the cell passes every 3 days achieving a cell confluence of 95% at 15 days were done.Results in cell confluence is obtained by counting cells suspended in the culture medium....

We performed a comparative analytical study in order to determine the preference it dentists and patients on the clinical use gel with the enzyme papain obtained from Carica pubescens versus conventional rotary method for the removal of tooth decay in children. The research began with pilot-scale production of enzyme gel remover Carica pubescens.To determine the preference of the removal of caries validated questionnaires were applied pretest and posttest to 308 dentists and 308 patients randomly selected children who participated voluntarily, from cities in southern Peru. The dentists were trained in patient selection, the diagnosis of tooth decay, the inclusion and exclusion criteria and clinical application technique of gel. At random was used gel or conventional rotary method at the mouth of a patient with similar clinical features of two oclusal carious lesions and at successive tim...

Se realizó un estudio experimental con dos productos: el Gel Elaborado en base a papaína contenida en el Extracto Hidroalcohólico de Carica papaya arequipensis al 10% (Gel Elaborado) y Gel Papacarie, compuesto por papaína al 2%, cloramina y azul de toluidina (Gel Control); con el propósito de evaluar su acción sobre el reblandecimiento y remoción de la caries dental en dientes de niños. Fueron consideradas III fases de investigación: la fase I consistió en la preparación del Gel Elaborado con pruebas de laboratorio de estabilización y almacenamiento. La Fase II fue un estudio previo in vitro en el cual se aplicó el Gel Elaborado y Gel Control en 23 dientes recientemente extraídos pertenecientes a niños. Cada diente fue dividido en dos unidades de análisis (Nº 46) y fueron designados al azar en grupo experimental (Nº 23), que recibió el Gel Elaborado y grupo control (N�...

OBJECTIVE: to evaluate the survival of high viscosity ionomers in molars with atraumatic restoration treatment at 3, 6, 9 and 12 months. MATERIAL AND METHODS: it was an interventional, observational and longitudinal study. Of 100 preschoolers of both sexes from a rural public school, the 30 preschoolers selected met the inclusion criteria; their parents agreed to sign the consent. The university UCSM gave the approval of the Ethics Committee with the number 2017-07. A USP senior pediatric dentist calibrated three pediatric dentists to unify the criteria of ionomer manipulation and atraumatic restoration treatment standardized by WHO, 12 with intra-specialist Kappa 0.9 and inter-specialist Kappa 0.8. The ionomers for the clinical technique were applied in 143 deciduous molars that met the inclusion criteria, the retention and survival of the ionomers were evaluated according to the criter...