This research develops a microbicidal aqueous paint to prevent infections on high-touch surfaces. Based on the experimental tests, two chemical formulations were proposed consisting of an acrylic latex resin, silicone resin, titanium oxide, zinc omazide, copper oxide nanoparticles and additives. Th e results showed that the formulation 2 paint presented good homogenization, gloss and stability, its average drying time is 30 min, it has adequate metal coating thickness (49.2 μm) and viscosity (60 s in Ford No. 4 cup), according to the standards ASTM D 1186 01 and ASTM D 1200, respectively; Based on these characteristics, it is considered to be of acceptable quality. Th e bioassays carried out with bacteria of Staphylococcus aureus Rosenbach, 1884 and Escherichia coli (Escherich, 1885) by the incorporation method, showed that the two formulations have antifungal and antimicrobial properti...