The objective of this study was to estimate the seroprevalence of antibodies against Anaplasma spp in students and staff of the Teaching Veterinary Clinical Hospital (HCVD) of the School of Veterinary Medicine of the University Santo Tomás (UST), Talca, Chile. Blood samples (n=58) were collected and analyzed by indirect immunofluorescence (IFI) for IgG. The exact Fischer or X2 test was used to evaluate significant differences (p<0.05) and the Kappa index to evaluate concordance between IFI and clinical signology. The results showed 25.9% of  seropositivity among students and staff for Anaplasma spp. Significant differences were found in the use of biosecurity measures, extraction of ticks, and ectoparasite findings in clothing. The Kappa index was 0.13.

The aim of this study was to determine the prevalence of Brucella canis in a population of stray canines using different diagnostic techniques available in Chile and to evaluate their level of concordance. Blood samples were taken and the testicles of 30 stray canines from a sector of the eastern zone of the city of Talca were collected, which were part of a sterilization campaign by the Chilean government. To consider an individual positive for B. canis, at least two positive serological techniques were considered. Clinical examination was performed, and blood samples were analyzed by commercial ELISA, immunochromatography (ICT), counterimmunoelectrophoresis (CIEF), indirect ELISA (ELISA-I) with rough lipopolysaccharide (LPS-R), as well as testicular histopathology. The results showed that 6.6% (2/30) of positive individuals, while the concordance, according to the Kappa analysis was wi...

El estudio tuvo como objetivo estimar la seroprevalencia de anticuerpos contra Anaplasma spp en estudiantes y personal del Hospital Clínico Veterinario Docente (HCVD) de la Escuela de Medicina Veterinaria de la Universidad Santo Tomás (UST), Talca, Chile. Se analizaron 58 muestras sanguíneas humanas mediante inmunofluorescencia indirecta (IFI) para IgG. Se utilizó el test exacto de Fischer o X2 para evaluar diferencias significativas (p<0.05) y el índice de Kappa para evaluar concordancia entre IFI y signología clínica. Se obtuvo el 25.9% de seropositividad a Anaplasma spp, encontrándose diferencias significativas en uso de medidas de bioseguridad, extracción de garrapatas y hallazgos de ectoparásitos en ropa. El índice Kappa fue de 0.13.