The aim of this study was to determine the prevalence of intestinal parasites in food handlers that attended the Metropolitan Municipality of Lima, Peru. A total of 217 stool samples were examined between July and August, 2011. Direct methods were used with Lugol, or centrifugation technique Ritchie formalin-ethyl acetate as concentration method and Ziehl-Neelsen modified for detection of coccidia. Of the total sample, 107 (49.3%) were males and 110 (50.7%) were females. The average age of the study population was 26.78 years-old. The overall prevalence of parasites was 71.89%. There was no statistically significant differences in the prevalence of parasitism in terms of gender and age of the study population. The higher prevalence of intestinal parasites was found in the age group of 18-27 years (46.1%). The most prevalent species of protozoa were: Blastocystis hominis (55.8%), Entamoeb...