Universidad Nacional Agraria La Molina. Facultad de Ciencias. Departamento Académico de Biología

Bartonella bacilliformis es el agente causante de la enfermedad de Carrión, una enfermedad tropical desatendida que afecta principalmente a personas que viven en la región andina de Perú y Ecuador. El diagnóstico serológico es el método más utilizado para detectar la infección por B. bacilliformis, pero las pruebas serológicas actuales tienen limitaciones en cuanto a sensibilidad y especificidad. Por lo tanto, existe la necesidad de desarrollar nuevas y más precisas herramientas de diagnóstico. Las proteínas recombinantes se han propuesto como candidatos prometedores para mejorar el diagnóstico serológico de la enfermedad de Carrión. En este estudio experimental, el objetivo es optimizar las condiciones para producir proteínas recombinantes de B. bacilliformis utilizando el sistema de expresión de baculovirus - células de insectos. Específicamente, evaluamos diferentes...

In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet) belonging to Agrarian Innovation National Institute (INIA) Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR) primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.

In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet) belonging to Agrarian Innovation National Institute (INIA) Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR) primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.