In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet) belonging to Agrarian Innovation National Institute (INIA) Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR) primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.

In order to analyze transferibility of 15 microsatellite primers from Fabaceae family to Lupinus mutabilis Sweet "andean lupine" chosen to present transferability between species and genera, by its high rate of polymorphic content (PIC) and high degree of observed and expected heterozygosity. DNA was extracted of 300 andean lupines plants, PCR conditions were standardized by gradients of master mix components. Primers for screening were run on 3% agarose gel with some samples. Finally population was amplified and run on 6% polyacrylamide gel for its highest resolution. Only 6.67% of primers were amplified, but they were monomorphic, so they cannot be used for molecular characterization. We proposed eight microsatellite primers for andean lupin wich should be probed in laboratory conditions.

In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet) belonging to Agrarian Innovation National Institute (INIA) Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR) primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.

In order to analyze transferibility of 15 microsatellite primers from Fabaceae family to Lupinus mutabilis Sweet "andean lupine" chosen to present transferability between species and genera, by its high rate of polymorphic content (PIC) and high degree of observed and expected heterozygosity. DNA was extracted of 300 andean lupines plants, PCR conditions were standardized by gradients of master mix components. Primers for screening were run on 3% agarose gel with some samples. Finally population was amplified and run on 6% polyacrylamide gel for its highest resolution. Only 6.67% of primers were amplified, but they were monomorphic, so they cannot be used for molecular characterization. We proposed eight microsatellite primers for andean lupin wich should be probed in laboratory conditions.