Capsicum chinense “ají supano” is an ecotype typical of the countryside of Supe veryappreciated in the regional cuisine for its aroma and flavor. This ecotype to date hasno studies, so the objective of the present study is to characterize it molecularly toidentify intragenotypic variability of 30 individuals of chili peppers from the lowerSupe river basin using molecular markers ISSR. Fruits were collected from fourfarmers during the month of November, 2016, being the seeds selected randomly,for their establishment in pots in house mesh. DNA was extracted from three youngleaves using the Micro - CTAB method modified by Biotechnology Lab of PIPS inCereals and Native Crops of UNALM to molecular analysis. Primers SSR 22, UCB807, UCB 810 and UCB 841 were selected due their high resolution and banding;which did not detect different bands among the individuals evaluated, confirming thatit...

Capsicum chinense “ají supano” is an ecotype typical of the countryside of Supe veryappreciated in the regional cuisine for its aroma and flavor. This ecotype to date hasno studies, so the objective of the present study is to characterize it molecularly toidentify intragenotypic variability of 30 individuals of chili peppers from the lowerSupe river basin using molecular markers ISSR. Fruits were collected from fourfarmers during the month of November, 2016, being the seeds selected randomly,for their establishment in pots in house mesh. DNA was extracted from three youngleaves using the Micro - CTAB method modified by Biotechnology Lab of PIPS inCereals and Native Crops of UNALM to molecular analysis. Primers SSR 22, UCB807, UCB 810 and UCB 841 were selected due their high resolution and banding;which did not detect different bands among the individuals evaluated, confirming thatit...

Capsicum chinense “ají supano” is an ecotype typical of the countryside of Supe veryappreciated in the regional cuisine for its aroma and flavor. This ecotype to date hasno studies, so the objective of the present study is to characterize it molecularly toidentify intragenotypic variability of 30 individuals of chili peppers from the lowerSupe river basin using molecular markers ISSR. Fruits were collected from fourfarmers during the month of November, 2016, being the seeds selected randomly,for their establishment in pots in house mesh. DNA was extracted from three youngleaves using the Micro - CTAB method modified by Biotechnology Lab of PIPS inCereals and Native Crops of UNALM to molecular analysis. Primers SSR 22, UCB807, UCB 810 and UCB 841 were selected due their high resolution and banding;which did not detect different bands among the individuals evaluated, confirming thatit...

Perezia coerulescens, a native peruvian species, is used as tranquilizer in folk medicine and considered vulnerable status due extensive collection for its commercialization. Then, the aim of this research was obtain somatic embryos as alternative for its multiplication.Leaves, roots, rhyzomes, small shoot (15 – 20 mm) and big shoot (25 – 30 mm) from plants culture in vitro were grown using MS½ culture medium with sucrose (2%), agaragar (0,75%), 16 hours of light, 16 – 20 ºC, supplemented with NAA (2 mg/L), 2, 4-D (0,2; 1 and 2 mg/L) and without hormone. It was found that NAA (2 mg/L) and 2, 4-D (2 mg/L) induced callogenesis in 100% of small shoot and big shoot at two months, likewise 2, 4-D (1 mg/L) induced 100% of callogenesis of small shoot and big shoot at four months. However, embryogenic callus 2, 4-D (2 mg/L) treatment was fenolized at four month. Considering callus size a...

Perezia coerulescens, a native peruvian species, is used as tranquilizer in folk medicine and considered vulnerable status due extensive collection for its commercialization. Then, the aim of this research was obtain somatic embryos as alternative for its multiplication.Leaves, roots, rhyzomes, small shoot (15 – 20 mm) and big shoot (25 – 30 mm) from plants culture in vitro were grown using MS½ culture medium with sucrose (2%), agaragar (0,75%), 16 hours of light, 16 – 20 ºC, supplemented with NAA (2 mg/L), 2, 4-D (0,2; 1 and 2 mg/L) and without hormone. It was found that NAA (2 mg/L) and 2, 4-D (2 mg/L) induced callogenesis in 100% of small shoot and big shoot at two months, likewise 2, 4-D (1 mg/L) induced 100% of callogenesis of small shoot and big shoot at four months. However, embryogenic callus 2, 4-D (2 mg/L) treatment was fenolized at four month. Considering callus size a...

Perezia coerulescens, a native peruvian species, is used as tranquilizer in folk medicine and considered vulnerable status due extensive collection for its commercialization. Then, the aim of this research was obtain somatic embryos as alternative for its multiplication.Leaves, roots, rhyzomes, small shoot (15 – 20 mm) and big shoot (25 – 30 mm) from plants culture in vitro were grown using MS½ culture medium with sucrose (2%), agaragar (0,75%), 16 hours of light, 16 – 20 ºC, supplemented with NAA (2 mg/L), 2, 4-D (0,2; 1 and 2 mg/L) and without hormone. It was found that NAA (2 mg/L) and 2, 4-D (2 mg/L) induced callogenesis in 100% of small shoot and big shoot at two months, likewise 2, 4-D (1 mg/L) induced 100% of callogenesis of small shoot and big shoot at four months. However, embryogenic callus 2, 4-D (2 mg/L) treatment was fenolized at four month. Considering callus size a...

Los estudios sobre ajíes nativos en el Perú son escasos, por lo que el objetivo de esta investigación fue analizar y determinar las propiedades fisicoquímicas y metabólicas presentes en los frutos del ají supano (Capsicum chinense Jacq.), con el fin de darle valor agregado a sus cualidades nutriceúticas y correlacionarlas con las posibilidades de su industrialización; para lo cual se tomaron muestras de frutos de diferentes estados de color, a nivel de pequeños agricultores de la Campiña de Supe. Se registró la longitud, masa y diámetro del fruto (análisis biométrico); epicarpio, pedicelo y semillas (porcentaje biométrico); porcentaje de ceniza, humedad, carbohidrato, grasa, proteína, fibra cruda y energía (características físico químicas); carotenoides por método espectrofotométrico; Capsaicina, dihidrocapsaicina y flavonoides por método cromatográfico (HPLC) y v...

Los estudios sobre ajíes nativos en el Perú son escasos, por lo que el objetivo de esta investigación fue analizar y determinar las propiedades fisicoquímicas y metabólicas presentes en los frutos del ají supano (Capsicum chinense Jacq.), con el fin de darle valor agregado a sus cualidades nutriceúticas y correlacionarlas con las posibilidades de su industrialización; para lo cual se tomaron muestras de frutos de diferentes estados de color, a nivel de pequeños agricultores de la Campiña de Supe. Se registró la longitud, masa y diámetro del fruto (análisis biométrico); epicarpio, pedicelo y semillas (porcentaje biométrico); porcentaje de ceniza, humedad, carbohidrato, grasa, proteína, fibra cruda y energía (características físico químicas); carotenoides por método espectrofotométrico; Capsaicina, dihidrocapsaicina y flavonoides por método cromatográfico (HPLC...

Los estudios sobre ajíes nativos en el Perú son escasos, por lo que el objetivo de esta investigación fue analizar y determinar las propiedades fisicoquímicas y metabólicas presentes en los frutos del ají supano (Capsicum chinense Jacq.), con el fin de darle valor agregado a sus cualidades nutriceúticas y correlacionarlas con las posibilidades de su industrialización; para lo cual se tomaron muestras de frutos de diferentes estados de color, a nivel de pequeños agricultores de la Campiña de Supe. Se registró la longitud, masa y diámetro del fruto (análisis biométrico); epicarpio, pedicelo y semillas (porcentaje biométrico); porcentaje de ceniza, humedad, carbohidrato, grasa, proteína, fibra cruda y energía (características físico químicas); carotenoides por método espectrofotométrico; Capsaicina, dihidrocapsaicina y flavonoides por método cromatográfico (HPLC...