The aim of this study was to establish reference values for serum levels of thyroid stimulating hormone (TSH) and free tetraiodothyronine (T4L) in adult dogs in the area of Caldas, Colombia, and possible differences due to sex. Blood samples were collected from 78 intact dogs after fasting (40 females and 38 males older than one year of age). The serum concentration of TSH and T4L levels were determined by enzymatic immunoassay using commercial kits. TSH values for females were 0.53 ± 0.96 μUI/ml (range: 0.02-4.54) and for males were 0.36 ± 0.27 μUI/ml (0.02-0.91). T4L values for females were 1.08 ± 0.40 ng/dl (0.52-2.09), and for males was 1.07 ± 0.39 ng/dl (0.5-2.18). None statistical difference was found between sexes for both TSH and T4L.

El objetivo del estudio fue establecer valores de referencia para las concentraciones séricas de la hormona estimulante de la tiroides (TSH) y tetrayodotironina libre (T4L) en caninos adultos de la zona de Caldas, Colombia y las posibles diferencias atribuidas al sexo. Las muestras de sangre se recolectaron de 78 caninos enteros (40 hembras y 38 machos mayores de un año), en ayunas, y se determinaron los niveles de TSH y T4L mediante inmunoensayo enzimático con kits comerciales. Los valores de TSH para las hembras fueron de 0.53 ± 0.96 μUI/ml (rango de 0.02 a 4.54) y para los machos de 0.36 ± 0.27 μUI/ml (0.02 a 0.91). Los valores de T4L para las hembras fueron de 1.08 ± 0.40 ng/dl (0.52 a 2.09) y para los machos de 1.07 ± 0.39 ng/dl (0.5 a 2.18). No hubo diferencia estadística entre sexos para TSH y T4L.

The aim of this study was to compare the Direct method and the Friedewald formula method for the determination of LDL cholesterol in dogs. Blood samples were collected in 185 fasted dogs without sex restriction. Serum was extracted and levels of LDL cholesterol were determined by the two methods. The mean, standard deviation, and minimum and maximum values by the Direct method were 49.2 ± 16.7, 14.6 and 96.0 mg/dl, respectively, and by the Friedewald method were 65.2 ± 31.5, 4.6 and 184.2 mg/dl, respectively, with significant difference between the methods (p<0.05). The Direct method is recommended for determining LDL cholesterol levels in dogs.

The aim of this study was to compare the Direct method and the Friedewald formula method for the determination of LDL cholesterol in dogs. Blood samples were collected in 185 fasted dogs without sex restriction. Serum was extracted and levels of LDL cholesterol were determined by the two methods. The mean, standard deviation, and minimum and maximum values by the Direct method were 49.2 ± 16.7, 14.6 and 96.0 mg/dl, respectively, and by the Friedewald method were 65.2 ± 31.5, 4.6 and 184.2 mg/dl, respectively, with significant difference between the methods (p<0.05). The Direct method is recommended for determining LDL cholesterol levels in dogs.