The methods used for lipid profile measurement in humans, a species with LDL (“Low-density lipoprotein”) pattern, can be unsuitable for the lipid profile determination in species with HDL (“High-density lipoprotein”) pattern. The objective of this study was to compare the precipitation method and the Friedewald method for the measurement of LDL cholesterol in a species with HDL pattern. Blood samples were collected after 24 h fasting in 200 horses of different ages and without discrimination by sex. After serum extraction, levels of LDL cholesterol were determined by both methods. The values obtained by the precipitation method were 32 ± 8.8 mg/dl and for the Friedewald method were 29 ± 7.7 mg/dl (p<0.05). Given the difference in values between methods, it is recommended the Friedewald method for determining levels of LDL cholesterol in the horse, a species with HDL pattern.

En el Volumen 24, Número 3, Páginas 277-282 de la RIVEP se publicó el artículo «Determinaciónde los niveles de colesterol LDL en una especie con patrón HDL», siendo el únicoautor José Henry Osorio, tal y como constaba en el trabajo originalmente enviado a la revista porel autor.No obstante, a solicitud del autor, se modifica la autoría y se hace de conocimiento públicoque los autores son los siguientes:José Henry Osorio, Luis Fernando Uribe Velázquez, Vivian Rocío Rangel, Johana Carmona,Lucy Marcela Barrera, Jazmín Vinazco, Yirli Johana Suárez, Eliana Zulay Cañas, Sandra LorenaGiraldo, Luisa Fernanda Loaiza, María Elena Álvarez.

The aim of this study was to compare the Direct method and the Friedewald formula method for the determination of LDL cholesterol in dogs. Blood samples were collected in 185 fasted dogs without sex restriction. Serum was extracted and levels of LDL cholesterol were determined by the two methods. The mean, standard deviation, and minimum and maximum values by the Direct method were 49.2 ± 16.7, 14.6 and 96.0 mg/dl, respectively, and by the Friedewald method were 65.2 ± 31.5, 4.6 and 184.2 mg/dl, respectively, with significant difference between the methods (p<0.05). The Direct method is recommended for determining LDL cholesterol levels in dogs.

The aim of this study was to establish reference values for serum levels of thyroid stimulating hormone (TSH) and free tetraiodothyronine (T4L) in adult dogs in the area of Caldas, Colombia, and possible differences due to sex. Blood samples were collected from 78 intact dogs after fasting (40 females and 38 males older than one year of age). The serum concentration of TSH and T4L levels were determined by enzymatic immunoassay using commercial kits. TSH values for females were 0.53 ± 0.96 μUI/ml (range: 0.02-4.54) and for males were 0.36 ± 0.27 μUI/ml (0.02-0.91). T4L values for females were 1.08 ± 0.40 ng/dl (0.52-2.09), and for males was 1.07 ± 0.39 ng/dl (0.5-2.18). None statistical difference was found between sexes for both TSH and T4L.

The aim of this study was to compare two methods (precipitate and Friedewald) for the determination of LDL cholesterol values in cattle. Blood samples were taken from 56 cattle in early morning. In the serum LDL cholesterol levels were determined by the precipitation method and the Friedewald formula. The results were analyzed using a oneway analysis of variance. The precipitated method reported values (mg/dl) of 14.33, 0.36, 59.11, 58.75 and 11.21 for average, minimum, maximum, range and standard deviation, respectively, and by the Friedewald method values (mg/dl) were 62.04, 13.68, 156.82, 143.14 and 32.97 respectively, with significant difference between the two methods for a confidence level of 95.0%. The results indicate that the use of the precipitation method for the determination of LDL cholesterol in cattle is not recommended.

The aim of this study was to compare the direct method with the precipitation method for the determination of LDL cholesterol in a species with a metabolic HDL pattern. Blood samples were taken from 56 dairy cows early in the day. The LDL cholesterol levels were determined in the serum by both methods using commercial kits. The results were statistically analyzed using a one-way analysis of variance. The direct method reported values (mg/dl) of 46.66, 6.50, 101.83, 95.33 and 24.15 for average, minimum, maximum, range and standard deviation, respectively, and using the precipitation method values (mg/dl) of 14.33, 0.36, 59.12, 58.76 and 11.21 respectively, with a statistically significant difference between these methods for a 95.0% confidence level. The results indicate that the direct method is the most reliable for the determination of LDL cholesterol levels.

The aim of this study was to determine the levels of leptin in broilers and laying hens by using a kit designed for human samples. Fasting blood samples were collected from 80 broilers of the Cobb 500 line of 4 weeks of age (40 females and 40 males) and 86 hens of 26 weeks of age (43 Lohmann Brown and 43 Hy-line). Leptin levels were determined by an ELISA sandwich, after validation of the kit for use in commercial poultry. The mean, minimum, maximum and standard deviation values ​​of leptin for broilers were 0.61, 0.12, 3.99 and 0.10 ng/ml, respectively, without significant difference between sexes. Laying hens reported a mean, minimum, maximum and standard deviation values ​​of 0.66, 0.16, 1.83 and 0.11 ng/ml, respectively, without significant difference between lines. Similarly, no statistical difference was found between broiler chickens and laying hens. The results of the stu...

The methods used for lipid profile measurement in humans, a species with LDL (“Low-density lipoprotein”) pattern, can be unsuitable for the lipid profile determination in species with HDL (“High-density lipoprotein”) pattern. The objective of this study was to compare the precipitation method and the Friedewald method for the measurement of LDL cholesterol in a species with HDL pattern. Blood samples were collected after 24 h fasting in 200 horses of different ages and without discrimination by sex. After serum extraction, levels of LDL cholesterol were determined by both methods. The values obtained by the precipitation method were 32 ± 8.8 mg/dl and for the Friedewald method were 29 ± 7.7 mg/dl (p<0.05). Given the difference in values between methods, it is recommended the Friedewald method for determining levels of LDL cholesterol in the horse, a species with HDL pattern.

En el Volumen 24, Número 3, Páginas 277-282 de la RIVEP se publicó el artículo «Determinaciónde los niveles de colesterol LDL en una especie con patrón HDL», siendo el únicoautor José Henry Osorio, tal y como constaba en el trabajo originalmente enviado a la revista porel autor.No obstante, a solicitud del autor, se modifica la autoría y se hace de conocimiento públicoque los autores son los siguientes:José Henry Osorio, Luis Fernando Uribe Velázquez, Vivian Rocío Rangel, Johana Carmona,Lucy Marcela Barrera, Jazmín Vinazco, Yirli Johana Suárez, Eliana Zulay Cañas, Sandra LorenaGiraldo, Luisa Fernanda Loaiza, María Elena Álvarez.

The aim of this study was to compare the Direct method and the Friedewald formula method for the determination of LDL cholesterol in dogs. Blood samples were collected in 185 fasted dogs without sex restriction. Serum was extracted and levels of LDL cholesterol were determined by the two methods. The mean, standard deviation, and minimum and maximum values by the Direct method were 49.2 ± 16.7, 14.6 and 96.0 mg/dl, respectively, and by the Friedewald method were 65.2 ± 31.5, 4.6 and 184.2 mg/dl, respectively, with significant difference between the methods (p<0.05). The Direct method is recommended for determining LDL cholesterol levels in dogs.

El objetivo del estudio fue establecer valores de referencia para las concentraciones séricas de la hormona estimulante de la tiroides (TSH) y tetrayodotironina libre (T4L) en caninos adultos de la zona de Caldas, Colombia y las posibles diferencias atribuidas al sexo. Las muestras de sangre se recolectaron de 78 caninos enteros (40 hembras y 38 machos mayores de un año), en ayunas, y se determinaron los niveles de TSH y T4L mediante inmunoensayo enzimático con kits comerciales. Los valores de TSH para las hembras fueron de 0.53 ± 0.96 μUI/ml (rango de 0.02 a 4.54) y para los machos de 0.36 ± 0.27 μUI/ml (0.02 a 0.91). Los valores de T4L para las hembras fueron de 1.08 ± 0.40 ng/dl (0.52 a 2.09) y para los machos de 1.07 ± 0.39 ng/dl (0.5 a 2.18). No hubo diferencia estadística entre sexos para TSH y T4L.

El objetivo de este estudio fue comparar dos métodos (precipitado y Friedewald) para la determinación de los valores de colesterol LDL en bovinos. Se tomaron 56 muestras de sangre de bovinos en las primeras horas de la mañana. Se determinaron los niveles de colesterol LDL en el suero mediante el método de precipitación y mediante la fórmula de Friedewald. Los resultados fueron analizados mediante un análisis de varianza de una vía. El método precipitado reportó valores (mg/dl) de 14.33, 0.36, 59.11, 58.75 y 11.21 para promedio, mínimo, máximo, rango y desviación estándar, respectivamente, y mediante el método de Friedewald se obtuvieron valores (mg/dl) de 62.04, 13.68, 156.82, 143.14 y 32.97, respectivamente, habiendo diferencia significativa entre estos métodos para un nivel de confianza del 95.0%. Los resultados indican que no es recomendable la utilización del método...

El objetivo del estudio fue comparar el método Directo con el método de Precipitación para la determinación de colesterol LDL en una especie con patrón metabólico HDL. Se tomaron muestras de sangre de 56 vacas lecheras a primera hora del día. Se determinaron los niveles de colesterol LDL en el suero mediante ambos métodos utilizando kits comerciales. Los resultados fueron analizados estadísticamente mediante un análisis de varianza de una vía. El método directo reportó valores en mg/dl de 46.66, 6.50, 101.83, 95.33 y 24.15 para promedio, mínimo, máximo, rango y desviación estándar, respectivamente y mediante el método de precipitación se obtuvieron valores (mg/dl) de 14.33, 0.36, 59.12, 58.76 y 11.21, respectivamente, habiendo diferencia estadísticamente significativa entre estos métodos para un nivel de confianza del 95.0%. Los resultados indican que el método dire...

The aim of this study was to determine the levels of leptin in broilers and laying hens by using a kit designed for human samples. Fasting blood samples were collected from 80 broilers of the Cobb 500 line of 4 weeks of age (40 females and 40 males) and 86 hens of 26 weeks of age (43 Lohmann Brown and 43 Hy-line). Leptin levels were determined by an ELISA sandwich, after validation of the kit for use in commercial poultry. The mean, minimum, maximum and standard deviation values ​​of leptin for broilers were 0.61, 0.12, 3.99 and 0.10 ng/ml, respectively, without significant difference between sexes. Laying hens reported a mean, minimum, maximum and standard deviation values ​​of 0.66, 0.16, 1.83 and 0.11 ng/ml, respectively, without significant difference between lines. Similarly, no statistical difference was found between broiler chickens and laying hens. The results of the stu...

This study measured and compared the levels of low and high-density lipoprotein cholesterol in broilers and laying hens to determine the type of lipoprotein in higher concentration, using an analytical method that cannot be affected by high concentrations of triglycerides. Blood samples were collected in 30 broilers (Cobb 500) of 35-day-old and in 40 laying hens (Hy-Line W-36) 26 weeks old. Cholesterol levels of low and high density lipoproteins were measured by direct methods based on detergents and N,N-Bis(4-sulfobutyl)-m-toluidine. The results showed that high density lipoprotein cholesterol was more abundant in both broilers (93.1 ± 16 vs. 52.2 ± 9.1 mg/dL) and laying hens (61.7 ± 15.4 vs. 49.1 ± 12.6 mg/dL). It was concluded that using direct methods based on detergents and N,N-Bis (4- sulfobutyl)-m-toluidine, the high density lipoprotein is the metabolic pattern of lipids in co...

This study measured and compared the levels of low and high-density lipoprotein cholesterol in broilers and laying hens to determine the type of lipoprotein in higher concentration, using an analytical method that cannot be affected by high concentrations of triglycerides. Blood samples were collected in 30 broilers (Cobb 500) of 35-day-old and in 40 laying hens (Hy-Line W-36) 26 weeks old. Cholesterol levels of low and high density lipoproteins were measured by direct methods based on detergents and N,N-Bis(4-sulfobutyl)-m-toluidine. The results showed that high density lipoprotein cholesterol was more abundant in both broilers (93.1 ± 16 vs. 52.2 ± 9.1 mg/dL) and laying hens (61.7 ± 15.4 vs. 49.1 ± 12.6 mg/dL). It was concluded that using direct methods based on detergents and N,N-Bis (4- sulfobutyl)-m-toluidine, the high density lipoprotein is the metabolic pattern of lipids in co...

Didelphidae family represents the majority of American marsupials distributed in South America. They are present in most type of habitats except in high mountains and deserts. They have different sizes and are described by some authors as medium and large size, while others indicate small and medium sizes. The present work contributes with a description ofDidelphys marsupialis located in the south west region of Colombia.

Didelphidae family represents the majority of American marsupials distributed in South America. They are present in most type of habitats except in high mountains and deserts. They have different sizes and are described by some authors as medium and large size, while others indicate small and medium sizes. The present work contributes with a description ofDidelphys marsupialis located in the south west region of Colombia.