Twenty canine ejaculates (2nd fraction) were obtained by digital manipulation with the purpose of evaluating the effect of two extenders on the preservation of canine spermatozoa stored at 4 °C. Each ejaculate was evaluated through a spermiogram and the hypo-osmotic swelling test (HOST). The ejaculates were fractioned and diluted in a rate of 1:3 using egg yolk + TRIS + glucose + antibiotic (EYT) (365 mOsm/kg, pH 6.8) and skimmed milk (0.5% fat, UHT-treated) + antibiotic (SM) (272 mOsm/kg, pH 6.8). Progressive motility (PM) was 95.7 ± 3.6% and the HOST response was 79.8 ± 6.6% in fresh semen. In order to compare the effect of the two extenders on the preservation of the potential fertility, the PM and HOST were evaluated after 24, 48, 72, and 96 hours of storage at 4 ºC. The values of PM and HOST for EYT were significantly higher compared to SM at all times (p<0.05). Nevertheless,...

The purpose of this study was to describe reproductive and clinical parameters as a result of dexamethasone administration in order to terminate unwanted pregnancy in bitches. Five pregnant adult bitches, clinically healthy, were used. Animals were orally administered 0.2 mg/kg of dexamethasone every 12 hours during 6 days and thereafter, the dose was gradually reduced till 0.02 mg/kg on day 10. Ultrasound scan, body temperature, plasma progesterone, and behaviour were recorded throughout the study. The gestation was satisfactorily interrupted in 4 out of 5 bitches, where absorption or abortion occurred between 7 to 16 days after the beginning of the treatment. In the remaining animal, the interruption was partial (absorption and birth of immature puppies). Body temperature felt 1.0 - 1.5 ºC prior to the absorption or abortion. Progesterone concentration descended at day 4 of treatment ...

Se describe el caso de un perro mestizo de ocho meses con evidencias de alteraciones en la diferenciación sexual. Se observó desarrollo incompleto del prepucio, micropene sin presencia de abertura uretral, escroto bífido y pseudovagina con presencia de abertura uretral. Se discuten los hallazgos y se postula un posible síndrome de feminización testicular con resultado de pseudohermafroditismo masculino.

The effect of adding autologous or heterologous prostatic fluid in canine sperm and incubated at 38 °C (thermoresistance) on sperm motility and spermatic vigor was evaluated.Fifteen ejaculates were collected from mature English bulldog by digital manipulation.The sperm fraction and the prostatic fractions were kept separated during the collection.Prostatic fluids were processed and stored at -18 °C. An spermiogram was done for each ejaculate and then distributed in three aliquots of 0.5 ml and added 1 ml of autologous prostatic fluid, heterologous prostatic fluid or saline solution. The samples were incubated at 38 °C for 60 minutes measuring the progressive motility and spermatic vigor at 0, 15, 30,45 and 60 minutes. The results showed a lineal decreased for both parameters during the evaluation period, and without statistical difference between treatments or times of incubation. It ...

The objetive of this study was to determine the prevalence of bovine tuberculosis in 461 animals raised in small and medium farms at Parinacochas district, departament of Ayacucho, using the tuberculine intradermoreaction test. All animals resulted negative to bovine tuberculosis sugesting the absence of the disease in the area.

The case of a 4.5 years old Bullmastiff bitch presented to the clinic with prolonged haemorragic vaginal discharge is described. A systemic involvement was not observed in the gynecological examination, the anestrus status was determined by vaginal cytology and measurement of the progesterone level in plasma (<1 ng/ml), and the presence of uterine content and increased uterine size was confirmed by ultrasonography. Besides, a mild regenerative anemia was determined. Based on a history of irregular estrous cycles an ovariohysterectomy was performed as treatment. In the evaluation of the uterus was observed the presence of blood in the uterine lumen and cysts in the mucosa, what led to the diagnosis of hematometra concomitant with cystic endometrial hyperplasia.

The present report describes and discuss the case of a French bulldog bitch, 2 years and 3 months old with a gestation of about 37 days and vaginal bleeding for nearly 12 days. Infection by B. canis was discarded by serological examination. Determination of serum progesterone through commercial kit showed a low value (2.5-5 ng/ml) so supportive therapy was instituted by micronized progesterone (100 mg/day) until the cessation of vaginal bleeding and maintenance of pregnancy. Progesterone value at this point showed higher values (>5 ng/ml). After day 42 of gestation, hormone supplementation was modified to 100 mg every 48 hours and stopped on day 58 of pregnancy. The female gave birth to a live puppy and other three were removed by cesarean section (two alive and one dead).

The aim of the study was to obtain and morphologically evaluate canine oocytes from females at different phases of the estrous cycle and to compare the proportions of oocytes potentially suitable for in vitro maturation (IVM). Ovaries of 24 bitches, 6 for each phase of the estrous cycle, were sliced to recover the oocytes. These were morphologically classified into fit and unfit oocytes for IVM. The largest proportion of oocytes fit for IVM (86.6%) was recovered from females in proestrus and estrus (p<0.01) and the largest quantity of oocytes fit for IVM per female (448.0 ± 52.7) was obtained in the estrus phase (p<0.01). It is concluded that the morphological assessment of oocytes indicated that the quality and quantity of oocytes recovered during the oestrus and proestrus phase of the oestrus cycle in terms of their potential use form IVM were better and higher than those obtain...

The hypoosmotic test is widely used for determining sperm quality in several animal species. The present study aimed to compare the effect of incubation of canine sperm into two hypoosmotic solutions (0 mOsm/l - HOST-s and 150 mOsm/l - HOST) on sperm viability. Fifteen ejaculates (2nd fraction) were obtained by digital manipulation from 10dogs and the spermiograms were considered as normals. A 5 μl aliquots per ejaculate were diluted in 45 μl of each of the two hypoosmotic solutions and incubated at 37 °C for 5 and 45 min respectively. Sperm viability was assessed using eosin-nigrosin staining. Results of the hypoosmotic test were 92.1 and 90.1% of dilated spermatozoa for HOST-s and HOST respectively (p<0.05) and the sperm viability was 66.5 and 78.3% of live spermatozoa for HOST-s and HOST respectively (p<0.01). It was concluded that thelower osmolality generates lower sperm vi...

Con el propósito de describir y comparar la citología testicular en perros adultos y geriátricos se seleccionaron para orquiectomía 10 perros adultos (2-6 años) y 10 geriátricos (>8 años) sin patologías testiculares aparentes. Una vez recolectados los testículos se realizó punción y aspiración con aguja fina (AAF), obteniéndose cuatro muestras por perro. Las muestras fueron teñidas con May-Grünwald Giemsa y estudiadas al microscopio óptico. En la descripción celular se reconocieron los componentes celulares de los túbulos seminíferos e intersticio, así como también células anormales. Destacó la abundancia celular, lisis celular, restos citoplasmáticos y fondos teñidos en la mayoría de las muestras. En ambos grupos se observaron células redondas, multinucleación, núcleos bien teñidos, en ocasiones desnudos y patrón de cromatina condensada. En perros adult...

The pregnancy in the bitch occurs between fertilization and whelping, and its length has clinical importance. The complex biology of the gametes and embryos influences the length of the gestation, where the physiological period fluctuates from 57 to 70 days. This wide range can be influenced by the referential methods used to estimate the starting day. Controlled natural mating or artificial insemination based on the detection of ovulation allows reducing the range from 62 to 64 days. A high impact obstetric condition is the gestation of only one puppy, which tends to be longer, representing high risk of dystocia. Given the clinical relevance of a suitable projection of the length of the gestation and the prediction of the whelping date, mathematical formulas to calculate the fetal age and the whelping day have been developed with the systematization of gestational ultrasound.

The animal health and welfare are tasks that should not be dissociated, and therefore they should be linked for a greater understanding and establishment of comprehensive health. Based on this, the importance of establishing reproductive health guidelines in intact adult dogs, through medical evidence, as well as investigating the parameters that value knowledge, attitude and practice in relation to responsible ownership. In the present study, surveys were conducted as a data collection technique to 50 dog keepers. In the reproductive health examination, higher frequencies of subclinical, prostate, and testicular abnormalities were identified in dogs older than 6 years of age as compared to younger animals (p<0.05). Prostate enlargement was observed in 75% of dogs. Regarding the knowledge and attitude in responsible pet ownership and reproductive health, the responses showed that they...

The effectiveness of a combined treatment of cloprostenol and cabergoline was evaluated for interruption of gestation in bitches. A clinical experience is reported in an English bulldog bitch with a gestation of 30 days. The treatment was administrated every 24 hours and consisted in 5 μg/kg of cabergoline, orally, followed 30 minutes later by 1 μg/kg of cloprostenol, via subcutaneous. Physiological constants, effects post administration, and ecographic evolution of the gestation was evaluated. Also, progesterone concentration was determined in two blood samples using a quantitative commercial kit. Changes in gestational vesicles were observed at day 3 of treatment, and a lower cardiac frequency (<180 bpm) were recorded on day 4. A fetal expulsion occurred few hours later. Ultrasound on day 5 showed absence of vesicles and fetuses. Serum progesterone concentration on day 3 was 1 to ...

Se evaluó la efectividad de un tratamiento combinado de cloprostenol y cabergolina como método de interrupción de la gestación en hembras caninas. Se describe el caso de una perra Bulldog Inglés con gestación estimada de 30 días. Cada 24 horas se aplicó 5 μ/kg de cabergolina, vía oral, seguido a los 30 min de 1μ/kg de cloprostenol, vía subcutánea. Se evaluaron constantes fisiológicas, efectos post administración y evolución ecográfica de la gestación; asimismo, se determinó la concentración de progesterona sérica utilizando un kit comercial cuantitativo. Cambios en las vesículas gestacionales se observaron en el día 3 del tratamiento, y en el día 4 se evidenció una frecuencia cardíaca disminuida (<180 lpm), y una expulsión fetal horas más tarde. El examen ecográfico del día 5 mostró ausencia de vesículas gestacionales y fetos. La progesterona sérica en...

Con el propósito de evaluar el efecto de dos diluyentes seminales sobre la fertilidad potencial de espermatozoides caninos conservados a 4 ºC, se obtuvieron 20 eyaculados mediante manipulación digital. Cada eyaculado fue evaluado a través de espermiograma y de la prueba hipoosmótica (HOST), para luego ser fraccionado y diluido en relación 1:3 con uno de los siguientes diluyentes: yema de huevo + TRIS (EYT) (365 mOsm/kg, pH 6.8) y leche descremada fluida UHT (LD) (272 mOsm/kg, pH 6.8). En el semen fresco, la motilidad progresiva (MP) fue 95.7 ± 3.6% y la respuesta a HOST de 79.8 ± 6.6%. Para comparar el efecto del diluyente sobre la preservación de la fertilidad potencial, se realizaron evaluaciones de MP e integridad de membrana con HOST a las 24, 48, 72 y 96 horas de refrigeración del semen, observándose mayores valores de MP y de espermatozoides dilatados en el diluyente EYT...

The purpose of this study was to describe reproductive and clinical parameters as aresult of dexamethasone administration in order to terminate unwanted pregnancy inbitches. Five pregnant adult bitches, clinically healthy, were used. Animals were orallyadministered 0.2 mg/kg of dexamethasone every 12 hours during 6 days and thereafter,the dose was gradually reduced till 0.02 mg/kg on day 10. Ultrasound scan, bodytemperature, plasma progesterone, and behaviour were recorded throughout the study.The gestation was satisfactorily interrupted in 4 out of 5 bitches, where absorption orabortion occurred between 7 to 16 days after the beginning of the treatment. In theremaining animal, the interruption was partial (absorption and birth of immature puppies).Body temperature felt 1.0 - 1.5 ºC prior to the absorption or abortion. Progesteroneconcentration descended at day 4 of treatment and absorp...

Se describe el caso de un perro mestizo de ocho meses con evidencias de alteraciones en la diferenciación sexual. Se observó desarrollo incompleto del prepucio, micropene sin presencia de abertura uretral, escroto bífido y pseudovagina con presencia de abertura uretral. Se discuten los hallazgos y se postula un posible síndrome de feminización testicular con resultado de pseudohermafroditismo masculino.

The effect of adding autologous or heterologous prostatic fluid in canine sperm and incubated at 38 °C (thermoresistance) on sperm motility and spermatic vigor was evaluated.Fifteen ejaculates were collected from mature English bulldog by digital manipulation.The sperm fraction and the prostatic fractions were kept separated during the collection.Prostatic fluids were processed and stored at -18 °C. An spermiogram was done for each ejaculate and then distributed in three aliquots of 0.5 ml and added 1 ml of autologous prostatic fluid, heterologous prostatic fluid or saline solution. The samples were incubated at 38 °C for 60 minutes measuring the progressive motility and spermatic vigor at 0, 15, 30,45 and 60 minutes. The results showed a lineal decreased for both parameters during the evaluation period, and without statistical difference between treatments or times of incubation. It ...

The objetive of this study was to determine the prevalence of bovine tuberculosis in 461 animals raised in small and medium farms at Parinacochas district, departament of Ayacucho, using the tuberculine intradermoreaction test. All animals resulted negative to bovine tuberculosis sugesting the absence of the disease in the area.

The case of a 4.5 years old Bullmastiff bitch presented to the clinic with prolonged haemorragic vaginal discharge is described. A systemic involvement was not observed in the gynecological examination, the anestrus status was determined by vaginal cytology and measurement of the progesterone level in plasma (<1 ng/ml), and the presence of uterine content and increased uterine size was confirmed by ultrasonography. Besides, a mild regenerative anemia was determined. Based on a history of irregular estrous cycles an ovariohysterectomy was performed as treatment. In the evaluation of the uterus was observed the presence of blood in the uterine lumen and cysts in the mucosa, what led to the diagnosis of hematometra concomitant with cystic endometrial hyperplasia.