1
artículo
Publicado 2013
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The effect of adding autologous or heterologous prostatic fluid in canine sperm and incubated at 38 °C (thermoresistance) on sperm motility and spermatic vigor was evaluated.Fifteen ejaculates were collected from mature English bulldog by digital manipulation.The sperm fraction and the prostatic fractions were kept separated during the collection.Prostatic fluids were processed and stored at -18 °C. An spermiogram was done for each ejaculate and then distributed in three aliquots of 0.5 ml and added 1 ml of autologous prostatic fluid, heterologous prostatic fluid or saline solution. The samples were incubated at 38 °C for 60 minutes measuring the progressive motility and spermatic vigor at 0, 15, 30,45 and 60 minutes. The results showed a lineal decreased for both parameters during the evaluation period, and without statistical difference between treatments or times of incubation. It ...
2
artículo
Publicado 2013
Enlace
Enlace
The effect of adding autologous or heterologous prostatic fluid in canine sperm and incubated at 38 °C (thermoresistance) on sperm motility and spermatic vigor was evaluated.Fifteen ejaculates were collected from mature English bulldog by digital manipulation.The sperm fraction and the prostatic fractions were kept separated during the collection.Prostatic fluids were processed and stored at -18 °C. An spermiogram was done for each ejaculate and then distributed in three aliquots of 0.5 ml and added 1 ml of autologous prostatic fluid, heterologous prostatic fluid or saline solution. The samples were incubated at 38 °C for 60 minutes measuring the progressive motility and spermatic vigor at 0, 15, 30,45 and 60 minutes. The results showed a lineal decreased for both parameters during the evaluation period, and without statistical difference between treatments or times of incubation. It ...