The method of Brown, based on adsorption chromatography on alumina columns , was applied to determine , for the first time in our environment , changes in the urinary excretion of estrone , 17B -estradiol and estriol, in a group of 8 normal women , between 18 and 38 years old, at three points in menstrual cycle : foliculor phase , ovulatory and luteal phase peak . The results agreed with those reported by several authors, using the same method or similar methods . Cyclic variations of three estrogen excretion give evidence of ovulation demonstrated in 6 cases ; in the remaining two could not be ruled out, although not definitively proven the possibility of anovulatory cycles . Estrogenic good correlation between variations and basal temperature curves and between estriol excretion and body weight was observed. In a case of testicular feminization , the dosages of estrogen correlated well...

By using the Brown's procedure the authors have measured the urinary excretion of estrone, estradiol and estriol in a group of 10 normal women. Two peaks of excretion were observed: the first corresponding to ovulation and a second one in the luteal phase; both were significantly different of the values obtained in the first phase of the cycle.

By using the Brown's procedure the authors have measured the urinary excretion of estrone, estradiol and estriol in a group of 10 normal women. Two peaks of excretion were observed: the first corresponding to ovulation and a second one in the luteal phase; both were significantly different of the values obtained in the first phase of the cycle.