Objective: To identify and characterize phytoconstituents flavonoids in the inflorescence of Brassica oleracea L. var. Botrytis "Cauliflower" by High performance liquid chromatography and Mass spectrometry. Biological material: Cauliflower collected in Simbal-Trujillo. Method: Qualitative identification of phytoconstituents present in the inflorescence by separation with increasing polarity solvents (ether, ethanol and water) by high performance liquid chromatography (HPLC) and mass spectra (MS / MS), characterization was performed on the products of flavonoids obtained after acid and alkaline hydrolysis. Results: The cauliflower inflorescences presented secondary metabolites as glucosinolates, steroids, saponins, tannins and flavonoids. In the analysis of HPLC-MS / MS was obtained retention time (Rt) of glycosylated flavonoids, identified as acylated derivatives ( R- CO-): Quercetin - 3...