Malacological surveys were carried out in lakes located near the São Francisco River or its tributaries in Iguatama, Minas Gerais, Brazil, between July 2009 and September 2010. Of 2.609 specimens of Biomphalaria straminea (Dunker, 1848) collected, 14 (0.54%) were infected by amphistomecercariae. Metacercariae obtained in solid subtract were used to perform experimental infection of AKR/J strain of mice. It was verified the presence of eggs of the parasite in the feces of the mice 30 days after infection. Adult parasites recovered from the intestinal caecum were identified as Zygocotyle lunata (Diesing, 1836). This is the first report of Z. lunata in B. straminea from Brazil.