Rattus norvegicus is the definitive host of Angiostrongylus cantonensis. The aim of this study was to determine the type of macroscopic alterations and histopathological findings in the central nervous system (CNS) of R. norvegicus infected with A. cantonensis. A total of 35 rats were used. Among them, 23 rats from an animal facility were experimentally infected with L3 larvae and 5 remained as controls (not infected), and 7 rats that were captured in the city of Guayaquil, Ecuador, whose natural infection was confirmed in the laboratory. Seven out of 23 artificially infected rats showed changes in behavior (walking in circles) after 29 days post infection (dpi). Three of them were euthanized at 30-33 dpi and the other four at 48-105 dpi; also, all experimental rats and the seven captured rats were euthanized. The brains were dissected, weighed, perfused with 10% formalin and histologica...

Rattus norvegicus is the definitive host of Angiostrongylus cantonensis. The aim of this study was to determine the type of macroscopic alterations and histopathological findings in the central nervous system (CNS) of R. norvegicus infected with A. cantonensis. A total of 35 rats were used. Among them, 23 rats from an animal facility were experimentally infected with L3 larvae and 5 remained as controls (not infected), and 7 rats that were captured in the city of Guayaquil, Ecuador, whose natural infection was confirmed in the laboratory. Seven out of 23 artificially infected rats showed changes in behavior (walking in circles) after 29 days post infection (dpi). Three of them were euthanized at 30-33 dpi and the other four at 48-105 dpi; also, all experimental rats and the seven captured rats were euthanized. The brains were dissected, weighed, perfused with 10% formalin and histologica...

Rats (Rattus sp) are the definitive hosts of the nematode Angiostrongylus cantonensis. The objective of this study was to identify the organs affected by the presence of A. cantonensis (adults) and the type of damage caused, as well as to establish the presence of microorganisms (bacteria and fungi) that could be found in these lesions. The study was conducted in 2014-2016 with 65 specimens (60 infected in the laboratory, which presented the larva L1 in their stools and 5 uninfected as a control group). Pulmonary and cardiac tissue samples were obtained on different post-infection days (dpi), stained with Gram and cultured on horse blood agar, MacConkey agar, Thioglycolate medium, Cystin medium Triptein Agar (CTA). Macro and microscopic alterations in pulmonary and cardiac tissue were observed, as well as microorganisms in lung lesions in both Gram and bacteriological cultures. Hardened ...

Las ratas (Rattus sp) son los hospederos definitivos del nematodo Angiostrongylus cantonensis. El objetivo de este estudio fue identificar los órganos afectados por la presencia de A. cantonensis (adultos) y el tipo de daño causado, así como establecer la presencia de microorganismos (bacterias y hongos) que podrían encontrarse en dichas lesiones. El estudio se realizó en 2014-2016 con 65 especímenes (60 infectadas en el laboratorio, que presentaron la larva L1 en sus deposiciones y 5 no infectadas como grupo control). Las muestras de tejido pulmonar y cardiaco fueron obtenidas en diferentes días pos-infección (dpi), teñidas con Gram y sembradas en agar sangre de caballo, agar MacConkey, medio Tioglicolato y, medio Cistina Tripteína Agar (CTA). Se observaron alteraciones macro y microscópicas en tejido pulmonar y cardiaco, así como microorganismos en lesiones pulmonares tanto...