Aisla, selecciona e identifica 100 cepas de Actinocycetos aeróbicos saprófitos con capacidad para producir sustancias antimicrobianas y que contienen ácido L - Diaminopimélico en sus paredes celulares procedentes de muestras de suelos del Jardín Botánico de la Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, de los jardines de Barranco y Surco, así como de terrenos agrícolas de Supe y Huaral al interior de Lima. Selecciona un medio de cultivo óptimo para la producción de sustancias antimicrobianas por fermentación. Utiliza el medio Arginina-Giyceroi-Sales (AGS) para los cultivos de aislamiento selectivo, previo tratamiento de humedecimiento y secado de las muestras. El 53% de cepas muestra actividad antibacteriana frente a bacterias Gram positivas y negativas. Los cultivos de 24 cepas bioactivas, en 4 medios de fermentación (A, B, E y F), par...

New Delhi-type carbapenemase-producing Klebsiella pneumoniae Metallo-beta-lactamase (NDM) generates a serious public health problem due to its mechanism of antibiotic resistance and rapid dissemination, causing high rates of morbidity and mortality in hospitalized patients when antibiotic therapy options are drained. The objective of the study was to observe the detection capacity of NDM carbapenemases in Klebsiella pneumoniae using the modified carbapenem inactivation method (mCIM). A cross-sectional study was carried out using clinical isolates from a level IV hospital in Lima (Perú) in the months of March to May 2018. It was worked with 40 clinical isolates of Klebsiella pneumoniae resistant to carbapenems, positive for the NDM gene and 15 clinical isolates of Klebsiella pneumoniae sensitive to carbapenems, negative for the NDM gene. The identification of the gene was carried out by ...

Identificaci6n, determinación y cuantificación de aflatoxinas producidas por hongos aflatoxigenicos en productos naturales, como alimentos naturales y medicamentos de origen vegetal. Utilizando el medio Agar Coco Oxitetraciclina (CAMO) donde se aprecia fluorescencia azul en el UV para determinar y cuantificar la aflatoxina se uso Elisa ligada, utilizando el fotocolorimetro EIH Neogen y el programa Logit donde los resultados se dan en ppb o ug/kg de aflatoxina.

The leaves of Lupinus C.P. Smith were colected from the highlands in Lima, it contains among other 5 flavonoids and 5 alkaloids.The flavonoid genistein was characterized by means of visible-UV spectroscopy, observing the bathochromic and hipsochomic movements of the spectra with sodium methoxide, aluminuin chloride, boric acid and sodium acetate reagents.The IR spectrum reveals the presence of the alkaloid lupenin or hydroxylupenin.Both the flavonoid and the alkaloid show a strong antibacterial activity and the alkaloid shows in addition of this property an antifungic activity.

«in vitro & in vivo» studies of fue Anti-A Experimental Paste, with the help of Sangre de grado and tricalcic phosphate, they have shown an excellent capacity germicide and reparative of the conjunctive tissue. The fibroblástic activity observed in most of the cases, probably generated by effects of the tricálcic phosphate, is the same one that showed a similar behavior in a previous investigation, inducing the reparative process of the bony defects. However, the application of the Anti-A Experimental Paste in human in posexodoncic ad that showed dilution with the saliva and lack of adhesion tissue, believe the necessity to continue with the investigation line with the purpose of improving their original properties, incorporating biocompatible additives. the study consisted on elaborating 8 pastes with diverse formulations with the help of Sangre de grado, tricálcic phosphate, r...

«in vitro & in vivo» studies of fue Anti-A Experimental Paste, with the help of Sangre de grado and tricalcic phosphate, they have shown an excellent capacity germicide and reparative of the conjunctive tissue. The fibroblástic activity observed in most of the cases, probably generated by effects of the tricálcic phosphate, is the same one that showed a similar behavior in a previous investigation, inducing the reparative process of the bony defects. However, the application of the Anti-A Experimental Paste in human in posexodoncic ad that showed dilution with the saliva and lack of adhesion tissue, believe the necessity to continue with the investigation line with the purpose of improving their original properties, incorporating biocompatible additives. the study consisted on elaborating 8 pastes with diverse formulations with the help of Sangre de grado, tricálcic phosphate, r...

Identificaci6n, determinación y cuantificación de aflatoxinas producidas por hongos aflatoxigenicos en productos naturales, como alimentos naturales y medicamentos de origen vegetal. Utilizando el medio Agar Coco Oxitetraciclina (CAMO) donde se aprecia fluorescencia azul en el UV para determinar y cuantificar la aflatoxina se uso Elisa ligada, utilizando el fotocolorimetro EIH Neogen y el programa Logit donde los resultados se dan en ppb o ug/kg de aflatoxina.

The leaves of Lupinus C.P. Smith were colected from the highlands in Lima, it contains among other 5 flavonoids and 5 alkaloids.The flavonoid genistein was characterized by means of visible-UV spectroscopy, observing the bathochromic and hipsochomic movements of the spectra with sodium methoxide, aluminuin chloride, boric acid and sodium acetate reagents.The IR spectrum reveals the presence of the alkaloid lupenin or hydroxylupenin.Both the flavonoid and the alkaloid show a strong antibacterial activity and the alkaloid shows in addition of this property an antifungic activity.

The study of the chemistry composition of the volatil oil of Ocimum micranthum Wild was determinatged by chromatographies and spectronics methods, metil-eugenol (52.20%) was gottern like the principal component, in addition to cariofileno (15.67%), β-elemento (5,15%). The essential oil of Ocimum micranthum Wild were screened for antimicrobial activities (more sensibility over fungus), the agar overlay technique was applied over differents bacterials and fungus like this; Kleibsiella pneumonae, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Shiguella sp., Aspergillus niger, aspergillus flavus, Aspergillus fumigatus and Penicllium sp.

The study of the chemistry composition of the volatil oil of Ocimum micranthum Wild was determinatged by chromatographies and spectronics methods, metil-eugenol (52.20%) was gottern like the principal component, in addition to cariofileno (15.67%), β-elemento (5,15%). The essential oil of Ocimum micranthum Wild were screened for antimicrobial activities (more sensibility over fungus), the agar overlay technique was applied over differents bacterials and fungus like this; Kleibsiella pneumonae, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Shiguella sp., Aspergillus niger, aspergillus flavus, Aspergillus fumigatus and Penicllium sp.

The final formulation that includes Sangre de grado watery extract, excipients and preservants; Pasta Terapéutica Anti-A (PTA) was denominated, that profit later to take place the tests of accelerated stability, every 30 days by space of three months, that consisted of conserving the bottles containing the formulation of a same lot of manufacture to room temperature and 4 º C of refrigeration and to evaluate the consistency, aspect, color, scent and bactericidal activity. This last one was made by means of the method of diffusion in agar and the technique of excavation plate culture, in front of positive bacteria negative Gram and Gram. The results were satisfactory for Gram positive, like Staphylococcus aureus ATCC 6538, Staphylococcus epidermides ATCC 12228 and Bacillus subtilis ATCC 6633, in the case of single Gram negative I am observed antimicrobial activity forehead a Escherichia...

The final formulation that includes Sangre de grado watery extract, excipients and preservants; Pasta Terapéutica Anti-A (PTA) was denominated, that profit later to take place the tests of accelerated stability, every 30 days by space of three months, that consisted of conserving the bottles containing the formulation of a same lot of manufacture to room temperature and 4 º C of refrigeration and to evaluate the consistency, aspect, color, scent and bactericidal activity. This last one was made by means of the method of diffusion in agar and the technique of excavation plate culture, in front of positive bacteria negative Gram and Gram. The results were satisfactory for Gram positive, like Staphylococcus aureus ATCC 6538, Staphylococcus epidermides ATCC 12228 and Bacillus subtilis ATCC 6633, in the case of single Gram negative I am observed antimicrobial activity forehead a Escherichia...