We report a green strategy for the extraction of lichen substances from Stereocaulon glareosum. This sustainable alternative does not use volatile toxic organic solvents, but it is assisted by microwave and is checked by UHPLC/ESI/MS/MS. Ionic liquids may provide a better alternative in the extraction of natural products from lichens. © Copyright © 2020 Calla-Quispe, Robles, Areche and Sepulveda.

The authors thank CONCYTEC (Convention 231-2015 FONDECYT) and FONDECYT REGULAR (Grant 1170871 and 1150745) for financial support.