The seminal plasma (SP) has enzymatic and non-enzymatic antioxidants with the function of protecting sperm from the harmful effects of oxidative stress. The aim of this study was to evaluate the antioxidant contribution of SP during the freezing of equine semen and its effect on post-thawing sperm quality. The semen of five horses was collected, the SP was separated and supplemented in proportions of 0% (Control), 10% (PS10) and 20% (PS20) to the sperm fraction in the freezing extender. The total antioxidant capacity (TAC) was evaluated by the oxygen radical trapping capacity (ORAC) and ferric reducing capacity (FRAP) methods, and the activity of SOD, GPx and catalase enzymes by spectrophotometry. Fifteen ejaculates were subjected to freezing and motility (MOT), vitality (EV), morphology (AM), functional membrane integrity (MI) and mitochondrial membrane potential (MMP) of the sperm were...

The seminal plasma (SP) has enzymatic and non-enzymatic antioxidants with the function of protecting sperm from the harmful effects of oxidative stress. The aim of this study was to evaluate the antioxidant contribution of SP during the freezing of equine semen and its effect on post-thawing sperm quality. The semen of five horses was collected, the SP was separated and supplemented in proportions of 0% (Control), 10% (PS10) and 20% (PS20) to the sperm fraction in the freezing extender. The total antioxidant capacity (TAC) was evaluated by the oxygen radical trapping capacity (ORAC) and ferric reducing capacity (FRAP) methods, and the activity of SOD, GPx and catalase enzymes by spectrophotometry. Fifteen ejaculates were subjected to freezing and motility (MOT), vitality (EV), morphology (AM), functional membrane integrity (MI) and mitochondrial membrane potential (MMP) of the sperm were...

Con datos hidrográficos de dos prospecciones, realizadas en marzo y setiembre 2014, se describe la hidrografía y estratificación en la bahía de Miraflores (BM) asociadas a condiciones cálidas y frías en ambos periodos. En marzo se presentó una condición estratificada y cálida, con máximos valores de frecuencia de Brunt Väisälä (FBV) y de vorticidad potencial (VP); asimismo la termoclina, haloclina y picnoclina fueron pronunciadas, debido principalmente a la mayor radiación solar recibida. En setiembre, la columna de agua fue más homogénea y fría con presencia de una capa de mezcla débil y somera. La distribución de la temperatura potencial, salinidad y densidad potencial tuvo una relación directa con la estacionalidad y con la configuración de la bahía en el aspecto espacial. Se espera que los resultados obtenidos en el presente estudio contribuyan a un conocimiento...