The objective of the study was to identify leukocyte populations in the postnatal thymus of alpacas under 60 days of age. Euthanasia was performed on six Huacaya alpacas in Huanta province, Ayacucho, Peru. Thymus samples were obtained, embedded in OCT and immediately frozen in liquid nitrogen and stored at -70 ºC until processing. Frozen sections were prepared and mounted with organosilane. Immunohistochemistry was performed with mouse primary antibody and rabbit secondary antibodies. The reaction was evidenced using the avidin-biotin-peroxidase complex and was counter-stained with hematoxylin. Specific monoclonal antibodies were used for the identification of leukocytic subpopulations of the thymus. Five visual fields were randomly evaluated at 400X. The distribution and density of the staining was classified as negative (-), inconstant (±), slight (+), moderate (++) and strong (...

The objective of the study was to identify leukocyte populations in the postnatal thymus of alpacas under 60 days of age. Euthanasia was performed on six Huacaya alpacas in Huanta province, Ayacucho, Peru. Thymus samples were obtained, embedded in OCT and immediately frozen in liquid nitrogen and stored at -70 ºC until processing. Frozen sections were prepared and mounted with organosilane. Immunohistochemistry was performed with mouse primary antibody and rabbit secondary antibodies. The reaction was evidenced using the avidin-biotin-peroxidase complex and was counter-stained with hematoxylin. Specific monoclonal antibodies were used for the identification of leukocytic subpopulations of the thymus. Five visual fields were randomly evaluated at 400X. The distribution and density of the staining was classified as negative (-), inconstant (±), slight (+), moderate (++) and strong (...

Fine-needle aspiration biopsy guided with trans-abdominal ultrasound is a safe and minimally invasive technique that is used to confirm the diagnosis of prostatic neoplasia. A variation of this technique is a fine-needle biopsy without aspiration in which negative pressure is not exerted when obtaining the material. Four cases with clinical and ultrasound alterations compatible with prostatic neoplasia were evaluated with ultrasound-guided fine-needle biopsy without aspiration as a diagnostic tool. The procedure was done with conventional ultrasound guidance. When the area of interest was found, an ultrasoundguided sample collection was performed using a 21G x 1½” needle. The sample was expelled through the needle with a syringe onto a glass slide and stained using Tinción 15®. Sample interpretation was based on the identification of tumor cells and general criteria of malignancy. U...

Fine-needle aspiration biopsy guided with trans-abdominal ultrasound is a safe and minimally invasive technique that is used to confirm the diagnosis of prostatic neoplasia. A variation of this technique is a fine-needle biopsy without aspiration in which negative pressure is not exerted when obtaining the material. Four cases with clinical and ultrasound alterations compatible with prostatic neoplasia were evaluated with ultrasound-guided fine-needle biopsy without aspiration as a diagnostic tool. The procedure was done with conventional ultrasound guidance. When the area of interest was found, an ultrasoundguided sample collection was performed using a 21G x 1½” needle. The sample was expelled through the needle with a syringe onto a glass slide and stained using Tinción 15®. Sample interpretation was based on the identification of tumor cells and general criteria of malignancy. U...