The aim of this study was to determine the effect of cryopreservation on acrosomal integrity in viable alpaca sperm. Samples from 46 alpaca testicles that had motility >30% and sperm concentration >50x106 sperm/ml were processed. Sperm recovered from the tail of the epididymis were separated into two 500 µl aliquots. The first aliquot was for the initial fresh evaluation and the second aliquot was frozen in straws and stored in liquid nitrogen until the evaluation of acrosomal integrity. For the evaluation of viability and acrosomal integrity, 100 µl of each sample were incubated with 2.5 µl of FITC-PSA (100 µg/ml) and 0.5 µl of propidium iodide (PI, 2.4 mM) for 10 min at 38 °C. Immediately afterwards, the samples were evaluated by flow cytometry, acquiring 10 000 events compatible with sperm per sample. FITC-PSA and PI were excited with a 488 nm laser, and fluorescence ...

The aim of this study was to determine the effect of cryopreservation on acrosomal integrity in viable alpaca sperm. Samples from 46 alpaca testicles that had motility >30% and sperm concentration >50x106 sperm/ml were processed. Sperm recovered from the tail of the epididymis were separated into two 500 µl aliquots. The first aliquot was for the initial fresh evaluation and the second aliquot was frozen in straws and stored in liquid nitrogen until the evaluation of acrosomal integrity. For the evaluation of viability and acrosomal integrity, 100 µl of each sample were incubated with 2.5 µl of FITC-PSA (100 µg/ml) and 0.5 µl of propidium iodide (PI, 2.4 mM) for 10 min at 38 °C. Immediately afterwards, the samples were evaluated by flow cytometry, acquiring 10 000 events compatible with sperm per sample. FITC-PSA and PI were excited with a 488 nm laser, and fluorescence ...

Determina el efecto de la criopreservación sobre la integridad acrosomal de los espermatozoides viables de alpaca recuperados del epidídimo, determinado mediante citometría de flujo. Se procesaron muestras de 46 testículos de alpaca obtenidos de Camal Municipal de Ninacaca, Pasco, que presentaron una motilidad ≥ 30% y concentración espermática ≥ 50x106 espermatozoides/mL, en promedio 46.6% y 61x106 espermatozoides/mL, respectivamente del total de las muestras obtenidas. Los espermatozoides se recuperaron de la cola del epidídimo con 1mL de dilutor a base de leche descremada, yema de huevo, fructuosa y DMA; separándose luego en 2 alícuotas de 500 μL. La primera alícuota se utilizó para la evaluación de la viabilidad e integridad acrosomal inicial y la segunda alícuota se congeló en pajillas mediante un sistema automático de criopreservación, para luego almacenarse en ...

The aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colour) and microscopic (sperm motility and concentration, using light field microscopy) characteristics were recorded. Additionally, sperm function tests were performed: sperm viability (using SYBR-14 and propidium iodide), mitochondrial membrane potential (MMP, using MitoTraker Deep Red FM), and acrosomal integrity (using FITC-PSA and propidium iodide) and analysed by flow cytometry with image analysis. The following values ​​were found (mean ± 95% CI): 6.1 ± 2.7 ml volume, opalescent colour; 66.7 ± 10.5% motility; 262.7 ± 20.4 x 106 sperm/ml sperm concentration; 77.7 ± 9.7% of sperm via...

The aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colour) and microscopic (sperm motility and concentration, using light field microscopy) characteristics were recorded. Additionally, sperm function tests were performed: sperm viability (using SYBR-14 and propidium iodide), mitochondrial membrane potential (MMP, using MitoTraker Deep Red FM), and acrosomal integrity (using FITC-PSA and propidium iodide) and analysed by flow cytometry with image analysis. The following values ​​were found (mean ± 95% CI): 6.1 ± 2.7 ml volume, opalescent colour; 66.7 ± 10.5% motility; 262.7 ± 20.4 x 106 sperm/ml sperm concentration; 77.7 ± 9.7% of sperm via...