Microbial inoculant based on beneficial bacteria is a complementary management tool to increase vegetable production. However, its integration with chemical fertilization has been poorly studied. The present study evaluated the effects of microbial inoculant separately and in combination with chemical fertilization on lettuce under greenhouse conditions. An experiment was performed in pots using a completely randomized design. The microbial inoculant was applied at three fertilization levels. Soil response evaluated variables were: available N and P, total soil bacteria, soil enzymatic activities, and N leaching. Agronomic variables measured were: aerial biomass, chlorophyll content, and nutrient foliar contents. Microbial inoculant had a significant impact on soil total bacteria concentration. The application of reduced chemical fertilization and microbial inoculant had a similar effect...

Microbial inoculant based on beneficial bacteria is a complementary management tool to increase vegetable production. However, its integration with chemical fertilization has been poorly studied. The present study evaluated the effects of microbial inoculant separately and in combination with chemical fertilization on lettuce under greenhouse conditions. An experiment was performed in pots using a completely randomized design. The microbial inoculant was applied at three fertilization levels. Soil response evaluated variables were: available N and P, total soil bacteria, soil enzymatic activities, and N leaching. Agronomic variables measured were: aerial biomass, chlorophyll content, and nutrient foliar contents. Microbial inoculant had a significant impact on soil total bacteria concentration. The application of reduced chemical fertilization and microbial inoculant had a similar effect...

Integrated Nutrition Management (INM) is a concept developed during the last decade in fruit agroecosystems based on the application of organic matter, biostimulants, and adjusted chemical fertilization to improve soil quality, plant rhizogenesis, and nutrition. Nevertheless, there are few studies on the application of the INM concept on the recovery of decayed orchards. The present study was focused on the evaluation of the effects of INM on soil and plant quality changes as recovery indicators of a decayed pear orchard. Two treatments (T1: conventional, T2: INM) were evaluated under three tree vigor levels, during two growing seasons. Old pear trees were used in a factorial experiment of two treatments (control and INM) x three vigor levels, based on a previous aerial Normalized Difference Vegetation Index (NDVI) image. Soil properties, nutrient leaf contents, and NDVI were evaluated. ...

The use of organic amendments is common under the concept of integrated nutrient management (INM) in Vitis vinifera (Table grape) to improve plant and soil quality. The objective of this study was to evaluate compost (C) and humic substances (HS) mixed with mineral fertilizer (MF) in an INM program of V. vinifera cv Thompson seedless. The chemical, biochemical and microbiological parameters were evaluated in soil on 1-year- old V. vinifera plants growing on Alfisol soil. Five treatments and control were evaluated: (T1) C+MF, (T2) HS+MF, (T3) C, (T4) HS, (T5) MF and (T6) absolute control. The results indicated that the application of C and HS, increased β glucosidase and dehydrogenase activities, reaching values of 90.2 μg p-nitrophenol g-1h-1 and 9.1 μg de TFP g-124h-1, respectively. In addition, pH was similar in all treatments while electrical conductivity increased with application...

The objective of this research was to evaluate extraction methods for β-glucosidases comparing three buffer solutions (MUB, acetate, and maleate) at different incubation times (0.5 h to 10 h) and in three different soil orders (Mollisols, Andisols and Ultisols). Seven acidic soils were evaluated, showing differences in pH, OM, and clay contents. To evaluate the effect of OM as enzymes source, one soil of each order was treated to partially remove its OM and then the enzyme assay was performed. When using MUB and maleate buffers the highest (32 and 31 µg-pNP g-soil-1h-1 in average, respectively) were found, and the latter was significantly (p < 0.050) correlated with the soil clay content. The activity obtained with acetate buffer was much lower (38.2 µg-pNP g-soil-1h-1 in average). The use of MUB buffer with 1 h of incubation is suggested as extraction method, showing good reprod...

The use of organic amendments is common under the concept of integrated nutrient management (INM) in Vitis vinifera (Table grape) to improve plant and soil quality. The objective of this study was to evaluate compost (C) and humic substances (HS) mixed with mineral fertilizer (MF) in an INM program of V. vinifera cv Thompson seedless. The chemical, biochemical and microbiological parameters were evaluated in soil on 1-year- old V. vinifera plants growing on Alfisol soil. Five treatments and control were evaluated: (T1) C+MF, (T2) HS+MF, (T3) C, (T4) HS, (T5) MF and (T6) absolute control. The results indicated that the application of C and HS, increased β glucosidase and dehydrogenase activities, reaching values of 90.2 μg p-nitrophenol g-1h-1 and 9.1 μg de TFP g-124h-1, respectively. In addition, pH was similar in all treatments while electrical conductivity increased with application...

The objective of this research was to evaluate extraction methods for β-glucosidases comparing three buffer solutions (MUB, acetate, and maleate) at different incubation times (0.5 h to 10 h) and in three different soil orders (Mollisols, Andisols and Ultisols). Seven acidic soils were evaluated, showing differences in pH, OM, and clay contents. To evaluate the effect of OM as enzymes source, one soil of each order was treated to partially remove its OM and then the enzyme assay was performed. When using MUB and maleate buffers the highest (32 and 31 µg-pNP g-soil-1h-1 in average, respectively) were found, and the latter was significantly (p < 0.050) correlated with the soil clay content. The activity obtained with acetate buffer was much lower (38.2 µg-pNP g-soil-1h-1 in average). The use of MUB buffer with 1 h of incubation is suggested as extraction method, showing good reprod...

Este estudio evaluó el efecto del manejo integrado de nutrición (MIN) en la disponibilidad de nutrientes y actividades enzimáticas del suelo durante la etapa de enraizamiento en Vitis vinífera. Los efectos de compost (C), fertilizante mineral (FM) e inoculante microbiano (IM) se evaluaron a través de siete tratamientos y un testigo en un diseño completamente al azar con cuatro unidades experimentales por tratamiento: T1 (C + IM + FM), T2 (C + FM), T3 (IM + FM), T4 (C + IM), T5 (FM), T6 (C), T7 (IM) y testigo (T). Indicadores químicos y bioquímicos se evaluaron a través de la prueba comparación de promedios (LSD, p ≤ 0,05). Los resultados indicaron que la aplicación de C y FM incrementó la concentración de P durante los primeros 30 días (64,8 y 69,9 mg kg-1), el N aumentó al día 60 en T1 (178,1 mg kg-1) y alcanzó su concentración máxima el día 30 en T2 (433,3 mg kg-1...

Este estudio evaluó el efecto del manejo integrado de nutrición (MIN) en la disponibilidad de nutrientes y actividades enzimáticas del suelo durante la etapa de enraizamiento en Vitis vinífera. Los efectos de compost (C), fertilizante mineral (FM) e inoculante microbiano (IM) se evaluaron a través de siete tratamientos y un testigo en un diseño completamente al azar con cuatro unidades experimentales por tratamiento: T1 (C + IM + FM), T2 (C + FM), T3 (IM + FM), T4 (C + IM), T5 (FM), T6 (C), T7 (IM) y testigo (T). Indicadores químicos y bioquímicos se evaluaron a través de la prueba comparación de promedios (LSD, p ≤ 0,05). Los resultados indicaron que la aplicación de C y FM incrementó la concentración de P durante los primeros 30 días (64,8 y 69,9 mg kg-1), el N aumentó al día 60 en T1 (178,1 mg kg-1) y alcanzó su concentración máxima el día 30 en T2 (433,3 mg kg-1...