This study evaluated the seasonal variability of water quality in the Tilacancha River, the water source that supplies Chachapoyas, and the rural communities of Levanto and San Isidro del Maino of Perú. Eighteen physical, chemical, and microbiological water parameters were evaluated at five sampling points in two seasons (rainy and dry). To determine water quality, the results obtained for the parameters evaluated were compared with the Maximum Permissible Limits (MPL) established in the Regulation on Water Quality for Human Consumption (DS Nº 031-2010-SA), approved by the Environmental Health Directorate of the Ministry of Health. In addition, a Pearson correlation was performed to estimate the correlation between the variables evaluated. The results showed that microbiological parameters exceeded the MPLs in both periods evaluated, such as the case of total coliforms (44 MPN.100 mL-1...